Bacterio- and lymphocytotoxicity of silver nanocomposite with sulfated arabinogalactan

Shurygina, I. A.; Шурыгин, М. Г.; Дмитриева, Л. А.; Фадеева, Т. В.; Ганенко, Т. В.; Танцырев, А. П.; Сапожников, А. Н.; Сухов, Б. Г.; Трофимов, Б. А.

doi:10.1007/s11172-015-1052-x

Cited by 9 publications

(4 citation statements)

References 12 publications

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“…When incubating human gingival fibroblast cells (CRL-2014) with AgNPs, it was found that AgNPs penetrated the cell membrane and localized inside the mitochondria [ 31 ]. In additional it was determined that AgNPs induced loss of cell viability and its ability to proliferation in a dose-dependent manner [ 18 , 32 , 33 , 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity

et al. 2020

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Novel silver/poly-1-vinyl-1,2,4-triazole nanocomposite materials—possessing antimicrobial activity against Gram-positive and Gram-negative bacteria—have been synthesized and characterized in the solid state and aqueous solution by complex of modern physical-chemical and biologic methods. TEM-monitoring has revealed the main stages of microbial cell (E. coli) destruction by novel nanocomposite. The concept of direct polarized destruction of microbes by nanosilver proposed by the authors allows the relationship between physicochemical and antimicrobial properties of novel nanocomposites. At the same time, it was shown that the nanocomposite was nontoxic to the fibroblast cell culture. Thus, the synthesized nanocomposite combining antibacterial activity against Gram-positive and Gram-negative bacteria as well as the absence of toxic effects on mammalian cells is a promising material for the development of catheters, coatings for medical devices.

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Section: Discussionmentioning

confidence: 99%

NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity

et al. 2020

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“…В последующие сутки скорость гибели снижалась, но, тем не менее, к концу эксперимента наблюдались лишь единичные клетки, не утратившие структурную целостность (рис. 5,6,7,8). рис.…”

Section: результаты и обсуждениеunclassified

“…При анализе фотодокументации было отмечено, что гибель клеток карциномы Эрлиха в процессе инкубации с веществом № 1 в концентрациях 20 мг/л и 10 мг/л обладала следующими особенностями: клетки активно гибли в 1-е сутки воздействия активным веществом, тогда как на 2-е сутки и далее снижение их числа носило постепенный характер (рис. 1,5,6,7,8). В то же время при воздействии вещества № 1 в концентрациях 5 мг/л и 2,5 мг/л клеточная гибель была более выражена в первые часы и достигала 50 % раньше, чем при воздействии более высоких концентраций.…”

Section: результаты и обсуждениеunclassified

“…Однако в последние десятилетия более актуальными становятся исследования, основанные на клеточных технологиях [1,2,3,4]. Работа с клеточной культурой, по сравнению с использованием животных, имеет ряд очевидных преимуществ, таких как отсутствие затрат на содержание животных, уменьшение длительности эксперимента, непосредственное наблюдение цитологического воздействия, возможность исследования механизмов, опосредующих токсический эффект тестируемого вещества, на клеточном уровне [5,6,7], а также возможность использования культур клеток человека, что позволяет нивелировать межвидовые различия на молекулярно-генетическом и рецепторно-сигнальном уровнях [5,6,8].…”

Section: Introductionunclassified

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Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT

Trukhan

Dremina

Lozovskaya

et al. 2019

Acta biomedica scientifica

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In recent decades, the methods based on the cell technologies have become more relevant for medical and pharmaceutical research. In this paper, weconsidered the BioStation CT using for continuous in vivo observation of Ehrlich ascitic carcinoma cell culture under the influence of two active substances in different concentrations (from 1.25 to 20 mg/l). As a result of the carcinoma cells condition monitoring for 4.5 days it was shown that substance No 2 does not affect the cell viability while substance No 1 causes the carcinoma cell death, and this active substance effect is dose-dependent. Photodocumentation at two-hour intervals allowed us to research differences in the rate of cell destruction (intensive disintegration in the first day with further stabilization of the living cells number or gradual cell death throughout the experiment), as well as differences in the time of 50 % mortality reaching. Thus in the study it was demonstrated that due to the fact that the BioStation CT combines the properties of a CO2 -incubator and a microscope this device is promising for toxicological studies and significantly expands the detection possibilities of processes occurring with living cells for a sufficiently long time period making possible further analysis of the cell behavior characteristics throughout the experiment, and that is fundamentally different from the systems allowing only the final result fixation of long-term active substance exposure.

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Selenium Nanocomposites in Diagnosis, Drug Delivery, and Treatment

Shurygina

Шурыгин

2020

Nanobiotechnology in Diagnosis, Drug Delivery, and Treatment

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Bacterio- and lymphocytotoxicity of silver nanocomposite with sulfated arabinogalactan

Cited by 9 publications

References 12 publications

NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity

NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity

Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT

Selenium Nanocomposites in Diagnosis, Drug Delivery, and Treatment

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