Bactericidal pathway of<i>Escherichia coli</i>in buffered saline treated with oxygen radicals

Kobayashi, Teiichi; Iwata, N.; Oh, Jun‐Seok; Hahizume, Hiroshi; Ohta, Takayuki; Takeda, Keigo; Ishikawa, Kenji; Hori, Masaru; Ito, Masafumi

doi:10.1088/1361-6463/aa61d7

Cited by 25 publications

(30 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A NEAPR source (Tough Plasma FPA10; Fuji MFG Co., Ltd.) was used to produce RAW. Deionized (DI) water (18.2 MΩ·cm at 25°C) was produced by a Millipore Direct‐Q 3UV system and treated using a radical source operated with a mixture of Ar, O 2 , and N 2 gases.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Investigation on the long‐term bactericidal effect and chemical composition of radical‐activated water

Iwata

Gamaleev

et al. 2019

Plasma Processes & Polymers

Self Cite

View full text Add to dashboard Cite

We treat deionized water using an atmospheric‐pressure radical source to investigate the role of neutral radicals in the bactericidal efficacy of radical‐activated water (RAW). The bactericidal efficacy of RAW is investigated using a colony‐forming unit assay with Escherichia coli. Both the bactericidal efficacy and reactive oxygen and nitrogen species (RONS) concentration in stored RAW are investigated over a period of a month. E. coli was completely sterilized even in RAW stored for 7 days, indicating the strong bactericidal effect. However, deterioration of the bactericidal efficacy of RAW stored for 14 days is confirmed, which does not have a clear correlation with the time evolution of the RONS concentration. This result becomes a strong evidence that RAW has long‐term bactericidal efficacy.

show abstract

Section: Methodsmentioning

confidence: 99%

“…An E. coli survival test was performed on each of the days described above to evaluate the bactericidal efficacy of RAW. The E. coli survival was estimated using the colony count method . E. coli (O1:K1:H7) was precultured in 3 ml nutrient broth (Difco™, BD) at 250 rpm with a regulated temperature of 30°C for 17 hr.…”

Section: Methodsmentioning

confidence: 99%

Investigation on the long‐term bactericidal effect and chemical composition of radical‐activated water

Iwata

Gamaleev

et al. 2019

Plasma Processes & Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plasma-activated liquid (PAL) including PAW is bacteriostatic against a number of microorganisms. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Its proposed high level of effectiveness in killing bacteria has earned it the nickname "water of death". 10) PAL has a good shelf-life with a reported retention of its antibacterial activity from days to weeks 7,15) to even years.…”

Section: Introductionmentioning

confidence: 99%

UV–vis spectroscopy study of plasma-activated water: Dependence of the chemical composition on plasma exposure time and treatment distance

Szili

Ogawa

et al. 2017

Jpn. J. Appl. Phys.

Self Cite

View full text Add to dashboard Cite

Plasma-activated water (PAW) is receiving much attention in biomedical applications because of its reported potent bactericidal properties. Reactive oxygen and nitrogen species (RONS) that are generated in water upon plasma exposure are thought to be the key components in PAW that destroy bacterial and cancer cells. In addition to developing applications for PAW, it is also necessary to better understand the RONS chemistry in PAW in order to tailor PAW to achieve a specific biological response. With this in mind, we previously developed a UV-vis spectroscopy method using an automated curve fitting routine to quantify the changes in H 2 O 2 , NO 2 % , NO 3 % (the major long-lived RONS in PAW), and O 2 concentrations. A major advantage of UV-vis is that it can take multiple measurements during plasma activation. We used the UV-vis procedure to accurately quantify the changes in the concentrations of these RONS and O 2 in PAW. However, we have not yet provided an in-depth commentary of how we perform the curve fitting procedure or its implications. Therefore, in this study, we provide greater detail of how we use the curve fitting routine to derive the RONS and O 2 concentrations in PAW. PAW was generated by treatment with a helium plasma jet. In addition, we employ UV-vis to study how the plasma jet exposure time and treatment distance affect the RONS chemistry and amount of O 2 dissolved in PAW. We show that the plasma jet exposure time principally affects the total RONS concentration, but not the relative ratios of RONS, whereas the treatment distance affects both the total RONS concentration and the relative RONS concentrations.

show abstract

“…The structure of the nozzle exit (a slit) with a bended flow channel downstream intercepts high‐energy photons, and the electrically grounded potential on the flow channel terminates charged species. In the previous studies on the oxygen radical source, a large number of the atomic oxygen O ( 3 P j ) was investigated and an absolute density on the order of 10 13 –10 14 cm −3 was measured by vacuum ultraviolet absorption spectroscopy . The radical source was employed to simplify the gaseous plasma‐liquid solution interaction, because it can selectively supply electrically neutral oxygen reactive species without charged species and high‐energy photons.…”

mentioning

confidence: 99%

“…The number of E. coli in oxygen radical activated and pristine solutions was analyzed at several time points using the CFU assay. Additional details about E. coli preculture can be found in a previous report …”

mentioning

confidence: 99%

Simultaneous achievement of antimicrobial property and plant growth promotion using plasma‐activated benzoic compound solution

Iwata

Gamaleev

Hashizume

et al. 2019

Plasma Processes & Polymers

Self Cite

View full text Add to dashboard Cite

This study assesses a potential use of the recently developed nonthermal atmospheric pressure plasma technology in hydroponic plant cultivation. Two types of plasma‐activated liquid solutions were compared, namely, l‐Phenylalanine (l‐Phe), which had a benzene ring structure, and l‐Alanine (l‐Ala), which did not have a benzene ring structure. Antimicrobial property and plant growth enhancement were simultaneously obtained when plasma‐activated l‐Phe was used. We found that the benzene ring structure in solution contributed to antimicrobial property. Further, colony forming unit assay indicated that 99% of Escherichia coli (E. coli) were eliminated after 24‐h incubation and radish sprout growth increased by about 40% after 2 days of cultivation.

show abstract

Bactericidal pathway ofEscherichia coliin buffered saline treated with oxygen radicals

Cited by 25 publications

References 37 publications

Investigation on the long‐term bactericidal effect and chemical composition of radical‐activated water

Investigation on the long‐term bactericidal effect and chemical composition of radical‐activated water

UV–vis spectroscopy study of plasma-activated water: Dependence of the chemical composition on plasma exposure time and treatment distance

Simultaneous achievement of antimicrobial property and plant growth promotion using plasma‐activated benzoic compound solution

Contact Info

Product

Resources

About