Bactericidal impact of Ag, ZnO and mixed AgZnO colloidal nanoparticles on HRv Mycobacterium tuberculosis phagocytized by THP-1 cell lines

Jafari, Alireza; Mosavari, Nader; Movahedzadeh, Farahnaz; Nodooshan, Saeedeh Jafari; Safarkar, Roya; Moro, Rossella; Kamalzadeh, Morteza; Majidpour, Ali; Boustanshenas, Mina; Mosavi, Tahereh

doi:10.1016/j.micpath.2017.07.010

Cited by 23 publications

(10 citation statements)

References 18 publications

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“…According to Jafari et al , mixed silver and zinc oxide nano colloidal particles had anti‐tubercular impact on H 37 Rv Mtb . They also fined that 40 ppm of rifampicin could not be able to eliminate 75 × 10 4 CFU ml −1 H 37 RvMtb after phagocytized by THP‐1 cell lines.…”

Section: Discussionmentioning

confidence: 99%

Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage

et al. 2017

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The World Health Organization acknowledges tuberculosis as a global threat.Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano-crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared. The MTT assay was employed to study the viability of human macrophage cell lines (THP-1) exposed to AgZnONCs. The bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were studied by Minimum Bactericidal Concentration (MBC) test. Subsequently, THP-1 were infected by H 37 Rv strain of M. tuberculosis (H 37 RvMtb). Also, bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were compared with ex-vivo conditions. The MBC of AgZnONCs and AgZnONCs+Rifampicin were ratios of 1:4 and 1:32 respectively (p-value <0.05). Also, more than 50% and 80% of THP-1 were alive in ratios of 1:4 and 1:32 in the presence of AgZnONCs, respectively. All phagocytic H 37 RvMtb were killed in the presence of AgZnONCs+Rifampicin (p-value <0.05),

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Section: Discussionmentioning

confidence: 99%

Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage

et al. 2017

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“…Nanomedicines have higher stability and increased circulation time, and improve targeted cargo delivery thereby resulting in enhanced efficacy while limiting drug toxicity (1). Numerous types of nanoparticles, including alginate (2), PLGA (3, 4, 5, 6, 7, 8), silver (9, 10), silver and zinc (11, 12), polymeric (13), gallium (III) (14), chitosan (15, 16), lipid (17), and silica (18) have been reported as drug delivery vehicles for therapeutics that target infectious diseases. For example, acrylate nanoparticles increased the intracellular concentration of anti-tuberculosis chemotherapeutics to macrophages compared to free drugs (19).…”

Section: Introductionmentioning

confidence: 99%

In vitro Pharmacokinetic Cell Culture System that Simulates Physiologic Drug and Nanoparticle Exposure to Macrophages

et al. 2019

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Purpose An in vitro dynamic pharmacokinetic (PK) cell culture system was developed to more precisely simulate physiologic nanoparticle/drug exposure. Methods A dynamic PK cell culture system was developed to more closely reflect physiologic nanoparticle/drug concentrations that are changing with time. Macrophages were cultured in standard static and PK cell culture systems with rifampin (RIF; 5 μg/ml) or β-glucan, chitosan coated, poly(lactic-co-glycolic) acid (GLU-CS-PLGA) nanoparticles (RIF equivalent 5 μg/ml) for 6 h. Intracellular RIF concentrations were measured by UPLC/MS. Antimicrobial activity against M. smegmatis was tested in both PK and static systems. Results The dynamic PK cell culture system mimics a one-compartment elimination pharmacokinetic profile to properly mimic in vivo extracellular exposure. GLU-CS-PLGA nanoparticles increased intracellular RIF concentration by 37% compared to free drug in the dynamic cell culture system. GLU-CS-PLGA nanoparticles decreased M. smegmatis colony forming units compared to free drug in the dynamic cell culture system. Conclusions The PK cell culture system developed herein enables more precise simulation of human PK exposure (i.e., drug dosing and drug elimination curves) based on previously obtained PK parameters.

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“…Previous studies have used different types of nanoparticles, either as drug carriers (Lu et al, 2018) or as bactericidal agents themselves against TB. In the latter case, the mycobactericidal potential of different metallic nanoparticles such as Ag (Selim et al, 2018), AgO, AgZnO (Jafari et al, 2017) or Ga (Choi et al, 2017) nanoparticles, has been evaluated.…”

Section: Discussionmentioning

confidence: 99%

Antimycobacterial Effect of Selenium Nanoparticles on Mycobacterium tuberculosis

et al. 2020

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Tuberculosis (TB) remains the leading cause of death from a single infection agent worldwide. In recent years, the occurrence of TB cases caused by drug-resistant strains has spread, and is expected to continue to grow. Therefore, the development of new alternative treatments to the use of antibiotics is highly important. In that sense, nanotechnology can play a very relevant role, due to the unique characteristics of nanoparticles. In fact, different types of nanoparticles have already been evaluated both as potential bactericides and as efficient drug delivery vehicles. In this work, the use of selenium nanoparticles (SeNPs) has been evaluated to inhibit the growth of two types of mycobacteria: Mycobacterium smegmatis (Msm) and Mycobacterium tuberculosis (Mtb). The results showed that SeNPs are able to inhibit the growth of both types of mycobacteria by damaging their cell envelope integrity. These results open a new opportunity for the use of this type of nanoparticles as antimycobacterial agents by themselves, or for the development of novel nanosystems that combine the action of these nanoparticles with other drugs.

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Bactericidal impact of Ag, ZnO and mixed AgZnO colloidal nanoparticles on HRv Mycobacterium tuberculosis phagocytized by THP-1 cell lines

Cited by 23 publications

References 18 publications

Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage

Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage

In vitro Pharmacokinetic Cell Culture System that Simulates Physiologic Drug and Nanoparticle Exposure to Macrophages

Antimycobacterial Effect of Selenium Nanoparticles on Mycobacterium tuberculosis

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