Bactericidal Efficacy of Hydrogen Peroxide‐Based Disinfectants Against Gram‐Positive and Gram‐Negative Bacteria on Stainless Steel Surfaces

Ríos-Castillo, Abel Guillermo; González-Rivas, Fabián; Jerez, José Juan Rodríguez

doi:10.1111/1750-3841.13790

Cited by 64 publications

(45 citation statements)

References 29 publications

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“…The size of the material used was 4.0 × 4.0 cm 2 , while the stainless steel surfaces consisted of discs with a diameter of 2.0 cm and a thickness of 1.2 mm. Prior to performing the study, the surfaces were cleaned, disinfected and autoclaved at 121°C for 15 min (Ríos‐Castillo, González‐Rivas, & Rodríguez‐Jerez, ), except the antibacterial plastic, which was only cleaned with 70% isopropyl alcohol (2‐propanol) before use so as not to interfere with its antibacterial properties.…”

Section: Methodsmentioning

confidence: 99%

Biofilm formation ofFlavobacterium psychrophilumon various substrates

et al. 2018

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The ability of Flavobacterium psychrophilum to adhere to and form biofilms on different types of materials used on rainbow trout (Oncorhynchus mykiss) farms was evaluated in this study. F. psychrophilum NCIMB 1947T, was inoculated onto a variety of different surfaces, including stainless steel, plastic, glass, wood, and zinc pyrithione encapsulated antibacterial plastic. The samples were then cultured in a humidified chamber or transferred into fish tanks containing either (1) freshwater or (2) filtered lake water. The formation of biofilms was quantified by fluorescent microscopy. F. psychrophilum formed biofilms on all of the surfaces tested; however, the adherence of the bacterium to the antibacterial plastic was much lower than the attachment observed on the other surfaces, illustrating the bacteriostatic properties of this material for F. psychrophilum. Moreover, bacterial numbers were greater on the surfaces maintained in lake water compared with those maintained in freshwater. The mineral composition of the lake water may have been responsible for the increased bacterial adherence observed between the two types of water. Treatment of the water, regular cleaning of equipment and the use of antimicrobial material to house the fish may help reduce biofilm formation by F. psychrophilum in fish farming systems.

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Section: Methodsmentioning

confidence: 99%

Biofilm formation ofFlavobacterium psychrophilumon various substrates

et al. 2018

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“…When the solution with just hydrogen peroxide was applied on E. coli, P. aeruginosa, and S. Typhimurium biofilms, bubble formations were detected, and it was considered as a positive reaction to the presence of biofilms. The election of hydrogen peroxide as an indicator of the presence of biofilms in this study was justified by the positive activity of this compound in different types of bacteria commonly found in food-processing environments reported by Elkins, Hassett, Stewart, Schweizer, and MCDermott (1999) ;Christensen, Trønnes, Vollan, Smidsrød, and Bakke (1990);and Ríos-Castillo et al (2017). When the solution of hydrogen peroxide that contained crystal violet stain was applied to the surfaces (Figure 3), the biofilm area stained blue and an intense bubble formation was observed, indicating that biofilms are more visual when colorants are included in the formula.…”

Section: Detection Of Biofilms Using Dem and The Different Biodetectomentioning

confidence: 82%

Development of a peroxide biodetector for a direct detection of biofilms produced by catalase-positive bacteria on food-contact surfaces

Ripolles‐Avila

Ríos-Castillo

Jerez

2018

CyTA - Journal of Food

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Detection of biofilms on food-contact surfaces is essential for establishing sanitization procedures and avoiding microbial contamination of food products. The objective of our study was to develop a peroxide biodetector, to detect and reveal the presence of biofilms. A positive reaction was observed when biodetector bubbles were formed on stainless steel and polypropylene surfaces. A range of microorganisms able to form biofilms was evaluated. Catalase-positive bacteria: Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium and Cronobacter sakazakii were employed as well as the catalase-negative bacterium: Lactobacillus brevis. With the exception of C. sakazakii, all catalase-positive foodborne pathogens forming the biofilms showed a positive detection by the biodetector, being 10 4 CFU cm −2 the minimum microbial load detected. The strongest positive reaction was for P. aeruginosa. Results demonstrated the potential of this biodetector to detect biofilms, particularly when used as a tool in the food industry.Desarrollo de un biodetector a base de peróxido para la detección directa de biofilms producidos por bacterias catalasa positivas en superficies de contacto con alimentos RESUMEN La detección de biofilms en superficies de contacto con alimentos es, hoy en día, esencial para establecer procedimientos de limpieza y desinfección eficaces y evitar la contaminación microbiana de productos alimenticios. El objetivo del presente estudio fue el desarrollo de un biodetector a base de peróxido, para detectar y revelar la presencia de biofilms mediante la formación de burbujas en superficies de acero inoxidable y polipropileno. Diferentes microorganismos fueron empleados en el estudio, entre ellos, bacterias catalasa positivas (Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium y Cronobacter sakazakii) y una bacteria catalasa negativa (Lactobacillus brevis). Con la excepción de C. sakazakii, el resto de patógenos mostraron una detección positiva por el biodetector, siendo 10 4 CFU cm −2 la mínima carga microbiana detectada. La reacción positiva más abundante fue para P. aeruginosa. Los resultados obtenidos demostraron el gran potencial que tiene el biodetector para la detección de biofilms, especialmente cuando éste se usa como herramienta en la industria alimentaria. ARTICLE HISTORY

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“…This efficacy was attributed to differences in the structure of the cell walls between Gram-negative and Gram-positive bacteria [60]. Other studies support this theory, noting that S. aureus tends to be more resistant to adverse conditions and to many types of disinfectants than Gram-negative bacteria [61][62][63]. Additionally, it has been suggested that the cell membrane structure is more complex in Gram-negative bacteria, making it more susceptible to environmental changes [64].…”

Section: Antimicrobial Activity Of Ag-nps Zno-nps and Ag-zno-nps On mentioning

confidence: 99%

Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

Fontecha‐Umaña

Ríos-Castillo

Ripolles‐Avila

et al. 2020

Foods

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Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions ≥ 2 log CFU/cm 2 ) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2.07 log CFU/cm 2 ). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4.90 log CFU/cm 2 ) and S. aureus (3.84 log CFU/cm 2 ). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5.80 log CFU/cm 2 ) and S. aureus (4.11 log CFU/cm 2 ). The results also showed a high correlation between concentration levels and the bacterial activity of Ag-ZnO-NPs (R 2 = 0.97 for S. aureus, and R 2 = 0.99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use.Foods 2020, 9, 442 2 of 15 studies include the development of antimicrobial surfaces capable of preventing and/or inhibiting bacterial adhesion [8][9][10][11].Metal agents such as silver, copper, zinc, titanium, and cobalt have antimicrobial properties. When these agents remain on surfaces, their biocidal properties are gradually released onto the surface through a process of ion exchange, providing continuous, lasting antimicrobial activity [12][13][14]. The biocidal properties of metal agents depend on the characteristics of their nanoparticles, such as size, distribution, morphology, and the release of very small quantities of their cations on the substrates on which they are acting [15,16]. The antimicrobial activity of these agents is also affected by extrinsic factors, such as the pH of the medium, time of exposure, concentration, UV radiation, and the presence of other substances or microorganisms [17][18][19].Two of the most extended nanocomposites for antibacterial purposes are silver and zinc oxides embedded in polymeric matrices [20][21][22]. The antimicrobial activity of silver (Ag) and zinc (Zn) repels or inhibits the initial attachment of bacteria by either exhibiting an anti-biofouling effect or by inactivating cells in contact with surfaces, causing their death [23,24]. Additionally, the antibacterial properties of these agents reduce the risk o...

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Bactericidal Efficacy of Hydrogen Peroxide‐Based Disinfectants Against Gram‐Positive and Gram‐Negative Bacteria on Stainless Steel Surfaces

Cited by 64 publications

References 29 publications

Biofilm formation ofFlavobacterium psychrophilumon various substrates

Biofilm formation ofFlavobacterium psychrophilumon various substrates

Development of a peroxide biodetector for a direct detection of biofilms produced by catalase-positive bacteria on food-contact surfaces

Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

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