Bactericidal Efficacy of Cold Atmospheric Plasma Treatment against Multidrug-Resistant Pseudomonas Aeruginosa

Abstract

Aim: The global of spread multidrug-resistant Pseudomonas aeruginosa has become a public health threat. Cold atmospheric plasma (CAP) is reported to have bactericidal efficacy; however, its effects on clinical super multidrug-resistant P. aeruginosa are unclear. The aim of this study was to investigate the bactericidal efficacy of CAP on a strain of super multidrug-resistant P. aeruginosa. Materials & methods: The effects of CAP treatments were evaluated using assays for the detection of growth, viability, metabolism, virulence factors and reactive oxygen species (ROS) levels. Results: Both CAP treatments dose-dependently inhibited cell viability and metabolic activity, and decreased the expression of several virulence factors. CAP treatment induced a significant increase in intracellular ROS levels, and ROS scavengers inhibited this effect. Conclusion: CAP treatment is a promising option for the clinical inhibition of multidrug-resistant P. aeruginosa, and the development of CAP technologies might be the key to solving the long-standing problem of drug-resistant bacteria.

Implementation of a Non-Thermal Atmospheric Pressure Plasma for Eradication of Plant Pathogens from a Surface of Economically Important Seeds

“…Furthermore, we herein noted electron transmission areas near the margins of DBD-treated D. solani IFB0099 cells (Figure 6). Analogous observations were made by Wang et al [37], who associated the presence of these areas with an outflow of the cytoplasm from all sites of the cell due to the rupturing of the cellular membrane. Moreover, our findings were confirmed by the study of Nishioka et al [13].…”

“…Similar high electron density zones to the ones presented in Figure 6 were observed by Lee et al [36] during studies on gram-positive (S. aureus and Streptococcus mutans) and gram-negative (Klebsiella oxytoca and Klebsiella pneumoniae) bacterial cells post-exposure to a non-thermal atmospheric pressure plasma jet on titanium discs. Wang et al [37] attributed the presence of high electron density regions in the plasma-treated bacterial cells to the accumulated chemicals, including ROS, RNS, and acidic conditions, which caused denaturation and aggregation of DNA fragments, proteins, and ribosomes [37]. Furthermore, we herein noted electron transmission areas near the margins of DBD-treated D. solani IFB0099 cells (Figure 6).…”

Effects of Plasma-Activated Water on Skin Wound Healing in Mice

“…The sterilization effect of PAW was incomplete when activating water for 1 or 3 min, but was effective with 5 min ( Figure 2 A,B). The ROS concentration in the PAW increases with the activation time, and CAP treatment destroys the integrity of the bacterial membrane [ 42 ], which may be due to the concentration of active particles in the PAW not being high enough to inactivate Pseudomonas aeruginosa when only using activation times of 1 and 3 min. The sterilization effect produced by activating water for 5 min was almost the same as the results after activating water for 10 min [ 42 ].…”

Antimicrobial Efficacy of Argon Cold Atmospheric Pressure Plasma Jet on Clinical Isolates of Multidrug-Resistant ESKAPE Bacteria

“…that plays a crucial role in the microbial inactivation by CAP [17]- [24]. The antimicrobial efficacy of CAP has been investigated invitro on various bacteria such as E. coli (from Family of Enterobacteria) [25]- [28], S. aureus [27]- [31], P. aeruginosa [28]- [33], K. pneumoniae [30], [31], A. baumannii [30], [34], etc. Further, CAP's antimicrobial efficacy has also been studied in-vivo [35]- [38], which initiates CAP's usage in clinical aspects.…”