Bactericidal effect of visible light in the presence of erythrosine on Porphyromonas gingivalis and Fusobacterium nucleatum compared with diode laser, an in vitro study

Habiboallah, Ghanbari; Mahdi, Zakeri; Mahbobeh, Naderi Nasab; Mina, Zareian Jahromi; Sina, Faghihi; Majid, Zakeri

doi:10.5978/islsm.14-or-20

Cited by 10 publications

(10 citation statements)

References 51 publications

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“…Song et al 33 compared the antimicrobial effects of blue light on periodontal pathogens in planktonic and biofilm conditions and noted that exposure to blue light for periodontal bacteria in the biofilm state is less effective than exposure in planktonic conditions. Habiboallah et al 34 studied the photodynamic sterilizing effect of visible light in the presence of erythrosine, a photosensitizer for gram-negative bacteria, and found that visible blue LED light (440 -480 nm, 570 mW) in conjugation with erythrosine significantly reduced bacterial viability. Thus, PDT alone may have limitations in the removal of bacteria and requires additional mechanical methods and photosensitizers.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Park

Kim

et al. 2019

J Dent Rehabil Appl Sci

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The purpose of this study was to evaluate the antimicrobial effects of a toothbrush with light-emitting diodes (LEDs) on periodontitis-associated dental biofilm attached to a zirconia surface by static and dynamic methods. Materials and Methods: Zirconia disks (12 mm diameter, 2.5 mm thickness) were inserted into a 24-well plate (static method) or inside a Center for Disease Control and Prevention (CDC) biofilm reactor (dynamic method) to form dental biofilms using Streptococcus gordonii and Fusobacterium nucleatum. The disks with biofilm were subdivided into five treatment groups-control, commercial photodynamic therapy (PDT), toothbrush alone (B), brush with LED (BL), and brush with LED+erythrosine (BLE). After treatment, the disks were agitated to detach the bacteria, and the resulting solutions were spread directly on selective agar. The number of viable bacteria and percentage of bacterial reduction were determined from colony counts. Scanning electron microscopy (SEM) was performed to visualize alterations in bacterial morphology. Results: No significant difference in biofilm formation was observed between dynamic and static methods. A significant difference was observed in the number of viable bacteria between the control and all experimental groups (P < 0.05). The percentage of bacterial reduction in the BLE group was significantly higher than in the other treated groups (P < 0.05). SEM revealed damaged bacterial cell walls in the PDT, BL, and BLE groups, but intact cell walls in the control and B groups. Conclusion: The findings suggest that an LED toothbrush with erythrosine is more effective than other treatments in reducing the viability of periodontitis-associated bacteria attached to zirconia in vitro.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Park

Kim

et al. 2019

J Dent Rehabil Appl Sci

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“…It has been shown in several scientific studies that the blue part of the visible spectrum (400-500 nm) is responsible for the suppression of various pathogens [21][22][23][24].…”

Section: Antiviral Properties Of Curcumin and Applicationsmentioning

confidence: 99%

Curcumin and Photobiomodulation in Chronic Viral Hepatitis and Hepatocellular Carcinoma

Ailioaie

Litscher

2020

IJMS

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Immune modulation is a very modern medical field for targeting viral infections. In the race to develop the best immune modulator against viruses, curcumin, as a natural product, is inexpensive, without side effects, and can stimulate very well certain areas of the human immune system. As a bright yellow component of turmeric spice, curcumin has been the subject of thousands of scientific and clinical studies in recent decades to prove its powerful antioxidant properties and anticancer effects. Curcumin has been shown to influence inter- and intracellular signaling pathways, with direct effects on gene expression of the antioxidant proteins and those that regulate the immunity. Experimental studies have shown that curcumin modulates several enzyme systems, reduces nitrosative stress, increases the antioxidant capacity, and decreases the lipid peroxidation, protecting against fatty liver pathogenesis and fibrotic changes. Hepatitis B virus (HBV) affects millions of people worldwide, having sometimes a dramatic evolution to chronic aggressive infection, cirrhosis, and hepatocellular carcinoma. All up-to-date treatments are limited, there is still a gap in the scientific knowledge, and a sterilization cure may not yet be possible with the removal of both covalently closed circular DNA (cccDNA) and the embedded HBV DNA. With a maximum light absorption at 420 nm, the cytotoxicity of curcumin as photosensitizer could be expanded by the intravenous blue laser blood irradiation (IVBLBI) or photobiomodulation in patients with chronic hepatitis B infection, Hepatitis B e-antigen (HBeAg)-positive, noncirrhotic, but nonresponsive to classical therapy. Photobiomodulation increases DNA repair by the biosynthesis of complex molecules with antioxidant properties, the outset of repairing enzyme systems and new phospholipids for regenerating the cell membranes. UltraBioavailable Curcumin and blue laser photobiomodulation could suppress the virus and control better the disease by reducing inflammation/fibrosis and stopping the progression of chronic hepatitis, reversing fibrosis, and diminishing the progression of cirrhosis, and decreasing the incidence of hepatocellular carcinoma. Photodynamic therapy with blue light and curcumin opens new avenues for the effective prevention and cure of chronic liver infections and hepatocellular carcinoma. Blue laser light and UltraBioavailable Curcumin could be a new valuable alternative for medical applications in chronic B viral hepatitis and hepatocarcinoma, saving millions of lives.

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“…Habiboallah et al [35] investigated the periodontal bactericidal effect on P. gingivalis and F. nucleatum using erythrosine and an LED (440-480 nm) compared with a diode laser (800 nm). Those authors concluded that erythrosine at 22 µM in the presence of a blue light and infrared diode laser could be considered as a potential approach of PDI against Gram-negative periodontal pathogenic species.…”

Section: Discussionmentioning

confidence: 99%

“…Those authors concluded that erythrosine at 22 µM in the presence of a blue light and infrared diode laser could be considered as a potential approach of PDI against Gram-negative periodontal pathogenic species. Nagata et al [36], in a review of PDI for dental caries, reported that erythrosine is the most hydrophilic photosensitizer and the Gram-positive bacteria allow better penetration of this dye, but Habiboallah et al [35] and Silva et al [37] showed good results with Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic inactivation of planktonic cultures of Streptococcus mutans using erythrosine irradiated by LED

Esper

Barbosa

Alvarenga

et al. 2020

BDS

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Objective: The aim of this in vitro study was to evaluate the efficacy of photodynamic inactivation (PDI) with erythrosine (E), using a light-emitting diode (LED) on planktonic cultures of Streptococcus mutans. Material and Methods: A Streptococcus mutans strain (UA 159) was used to prepare the suspensions containing 107 cells/mL, which was tested under different experimental conditions: a) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); b) LED irradiation only (P-L+); c) treatment with erythrosine only (E+L-); and d) no LED irradiation or photosensitizer (P) treatment, which served as a control group (P-L-). After treatment, strains were seeded onto MSBS agar for determination of the number of colony-forming units (CFU/mL). Results: The results were submitted to analysis of variance and the Tukey test (p < 0.05). No reduction in the number of CFU/mL was observed in the treatment group with erythrosine (E+L+) when compared to the control (P-L-). Conclusion: PDI using erythrosine did not reduce the number of CFUs per millimeter within the parameters in this study.KEYWORDSErythrosine; Decay; Photodynamic inactivation; Light-emitting diode.

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Bactericidal effect of visible light in the presence of erythrosine on Porphyromonas gingivalis and Fusobacterium nucleatum compared with diode laser, an in vitro study

Cited by 10 publications

References 51 publications

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Curcumin and Photobiomodulation in Chronic Viral Hepatitis and Hepatocellular Carcinoma

Photodynamic inactivation of planktonic cultures of Streptococcus mutans using erythrosine irradiated by LED

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