Bacterial transporters for sulfate and organosulfur compounds

Kertesz, Michael A.

doi:10.1016/s0923-2508(01)01199-8

Cited by 96 publications

(93 citation statements)

References 57 publications

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“…1 Pathway of assimilatory deamination of taurine-nitrogen by Klebsiella oxytoca TauN1. It is axiomatic that sulfonates require transport into the cell (Graham et al 2002), and we presume that either an ATP-binding cassette or a tripartite ATP-independent system (I) is involved in transporting taurine into the cell (Kertesz 2001;Bru¨ggemann et al 2004). Evidence is provided in the text for the presence of taurine:pyruvate aminotransferase (II), alanine dehydrogenase (III) and isethionate dehydrogenase (IV).…”

Section: Methodsmentioning

confidence: 99%

Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1

2005

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Klebsiella oxytoca TauN1 represents a group of isolates which utilise taurine (2-aminoethanesulfonate) quantitatively as a sole source of combined nitrogen for aerobic growth. During growth, a compound is excreted, which has now been identified as isethionate (2-hydroxyethanesulfonate). An ion-chromatographic separation of isethionate was developed to quantify the putative isethionate, whose identity was confirmed by matrix-assisted, laser-desorption ionisation time-offlight mass spectrometry. Strain TauN1 utilised taurine (and excreted isethionate) concomitantly with growth. Cell-free extracts contained inducible taurine transaminase, which yielded sulfoacetaldehyde. A soluble, NADP-dependent isethionate dehydrogenase converted sulfoacetaldehyde to isethionate. The enzyme was partially purified and it apparently belonged to the family of short-chain alcohol dehydrogenases.

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Section: Methodsmentioning

confidence: 99%

Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1

2005

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“…1). Evidence for both ATP-binding-cassette transporters and TRAP-transporters for the uptake of taurine has been presented (Brüggemann et al 2004;Kertesz 2001). However, since no significant homologues were found in the genome of R. palustris, the natures of the up- take systems for taurine and the exporter of sulfoacetate are open to conjecture (Fig.…”

Section: Enzymes Detected and The Transformation Pathwaymentioning

confidence: 99%

Sulfoacetate generated by Rhodopseudomonas palustris from taurine

et al. 2004

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Genes thought to encode (a) the regulator of taurine catabolism under carbon-limiting or nitrogen-limiting conditions and (b) taurine dehydrogenase were found in the genome of Rhodopseudomonas palustris. The organism utilized taurine quantitatively as a sole source of nitrogen (but not of carbon) for aerobic and photoheterotrophic growth. No sulfate was released, and the C-sulfonate bond was recovered stoichiometrically as sulfoacetate, which was identified by mass spectrometry. An inducible sulfoacetaldehyde dehydrogenase was detected. R. palustris thus contains a pathway to generate a natural product that was previously believed to be formed solely from sulfoquinovose.

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“…The product of sbp binds either sulphate or molybdate, and CysP recruits thiosulphate, while CysT and CysW are the permeases for sulphate/molybdate transport into the cell, and CysA has ATPase activity (Sirko et al, 1990;Kertesz, 2001). Since a double modA cysA mutant is still able to take up molybdate, a third transport system is involved in molybdate uptake in E. coli .…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of the Bradyrhizobium japonicum modA and modB genes involved in molybdenum transport

et al. 2006

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A modABC gene cluster that encodes an ABC-type, high-affinity molybdate transporter from Bradyrhizobium japonicum has been isolated and characterized. B. japonicum modA and modB mutant strains were unable to grow aerobically or anaerobically with nitrate as nitrogen source or as respiratory substrate, respectively, and lacked nitrate reductase activity. The nitrogen-fixing ability of the mod mutants in symbiotic association with soybean plants grown in a Mo-deficient mineral solution was severely impaired. Addition of molybdate to the bacterial growth medium or to the plant mineral solution fully restored the wild-type phenotype. Because the amount of molybdate required for suppression of the mutant phenotype either under free-living or under symbiotic conditions was dependent on sulphate concentration, it is likely that a sulphate transporter is also involved in Mo uptake in B. japonicum. The promoter region of the modABC genes has been characterized by primer extension. Reverse transcription and expression of a transcriptional fusion, P modA -lacZ, was detected only in a B. japonicum modA mutant grown in a medium without molybdate supplementation. These findings indicate that transcription of the B. japonicum modABC genes is repressed by molybdate.

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Bacterial transporters for sulfate and organosulfur compounds

Cited by 96 publications

References 57 publications

Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1

Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1

Sulfoacetate generated by Rhodopseudomonas palustris from taurine

Functional characterization of the Bradyrhizobium japonicum modA and modB genes involved in molybdenum transport

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