Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1

Rekowski, Katha rina Styp von; Denger, Karin; Cook, Alasdair M.

doi:10.1007/s00203-005-0776-7

Cited by 27 publications

(24 citation statements)

References 41 publications

(58 reference statements)

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“…Taurine was derivatized with dinitrofluorobenzene and quantified after separation by HPLC (Denger et al, 1997). Isethionate and sulfoacetate were determined by ion chromatography Styp von Rekowski et al, 2005). Sulfite was quantified as the fuchsin derivative (Denger & Cook, 2001).…”

Section: Methodsmentioning

confidence: 99%

The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates

et al. 2007

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Section: Methodsmentioning

confidence: 99%

The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates

et al. 2007

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“…The source of this isethionate was considered to be the gut flora (11), and a pathway of isethionate formation from taurine (2-aminoethanesulfonate) in bacteria was established (28). The compound is found in large amounts (at about 250 mM) in red algae (for an example, see reference 13) and on orb spiders' webs (ϳ2 M) (30).…”

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Gene Clusters Involved in Isethionate Degradation by Terrestrial and Marine Bacteria

Weinitschke

Sharma

Stingl

et al. 2010

Appl Environ Microbiol

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Ubiquitous isethionate (2-hydroxyethanesulfonate) is dissimilated by diverse bacteria. Growth of Cupriavidus necator H16 with isethionate was observed, as was inducible membrane-bound isethionate dehydrogenase (IseJ) and inducible transcription of the genes predicted to encode IseJ and a transporter (IseU). Biodiversity in isethionate transport genes was observed and investigated by transcription experiments.

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“…Stained protein bands were cut out of the gel and subjected to peptide mass fingerprinting to identify the corresponding genes, which was done under contract at TopLab (Martinsried, Germany). Sulfolactate, sulfopyruvate, and isethionate were quantified by ion chromatography with suppression (15,50). Sulfate release during growth was measured as turbidity of a suspension of insoluble BaSO 4 (49); a 1:10 dilution of the sample was necessary to avoid a precipitate with other components of the medium.…”

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Bifurcated Degradative Pathway of 3-Sulfolactate in Roseovarius nubinhibens ISM via Sulfoacetaldehyde Acetyltransferase and ( S )-Cysteate Sulfolyase

et al. 2009

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Data from the genome sequence of the aerobic, marine bacterium Roseovarius nubinhibens ISM were interpreted such that 3-sulfolactate would be degraded as a sole source of carbon and energy for growth via a novel bifurcated pathway including two known desulfonative enzymes, sulfoacetaldehyde acetyltransferase (EC 2.3.3.15) (Xsc) and cysteate sulfo-lyase (EC 4.4.1.25) (CuyA). Strain ISM utilized sulfolactate quantitatively with stoichiometric excretion of the sulfonate sulfur as sulfate. A combination of enzyme assays, analytical chemistry, enzyme purification, peptide mass fingerprinting, and reverse transcription-PCR data supported the presence of an inducible, tripartite sulfolactate uptake system (SlcHFG), and a membrane-bound sulfolactate dehydrogenase (SlcD) which generated 3-sulfopyruvate, the point of bifurcation. 3-Sulfopyruvate was in part decarboxylated by 3-sulfopyruvate decarboxylase (EC 4.1.1.79) (ComDE), which was purified. The sulfoacetaldehyde that was formed was desulfonated by Xsc, which was identified, and the acetyl phosphate was converted to acetyl-coenzyme A by phosphate acetyltransferase (Pta). The other portion of the 3-sulfopyruvate was transaminated to (S)-cysteate, which was desulfonated by CuyA, which was identified. The sulfite that was formed was presumably exported by CuyZ (TC 9.B.7.1.1 in the transport classification system), and a periplasmic sulfite dehydrogenase is presumed. Bioinformatic analyses indicated that transporter SlcHFG is rare but that SlcD is involved in three different combinations of pathways, the bifurcated pathway shown here, via CuyA alone, and via Xsc alone. This novel pathway involves ComDE in biodegradation, whereas it was discovered in the biosynthesis of coenzyme M. The different pathways of desulfonation of sulfolactate presumably represent final steps in the biodegradation of sulfoquinovose (and exudates derived from it) in marine and aquatic environments.Sulfolactate (Fig. 1A) is a widespread natural product, which contains the stable C-SO 3 Ϫ bond. The compound is known to be (i) a component (5% of dry weight) of bacterial endospores (5), (ii) an intermediate in the biosynthesis of coenzyme M in archaea (55), (iii) in equilibrium with (S)-cysteate in mammals (54), (iv) involved in the metabolism of sulfoquinovose (6-deoxy-6-sulfo-D-glucopyranose, the polar moiety of the plant sulfolipid) in plants and algae (e.g., see reference 48), and (v) an intermediate in the bacterial degradation of sulfoquinovose (44).Research on the biodegradation of organosulfonates has concentrated on compounds containing one to four carbon atoms (C 1 , C 2 , C 3 , or C 4 sulfonates), because where appropriate, larger molecules all seemed to be processed via one of the five desulfonative reactions that have been elucidated. Pathways from (i) sulfoquinovose yield, e.g., sulfoacetate or sulfolactate and 2,3-dihydroxy-1-sulfopropane (36, 44), (ii) taurocholate and N-acetyltaurine yield taurine (37, 43), and (iii) N-methyltaurine yield sulfoacetaldehyde (52). The five desulfonatio...

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Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1

Cited by 27 publications

References 41 publications

The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates

The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates

Gene Clusters Involved in Isethionate Degradation by Terrestrial and Marine Bacteria

Bifurcated Degradative Pathway of 3-Sulfolactate in Roseovarius nubinhibens ISM via Sulfoacetaldehyde Acetyltransferase and ( S )-Cysteate Sulfolyase

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