Bacterial transport of sulfate, molybdate, and related oxyanions

Aguilar-Barajas, Esther; Díaz-Pérez, César; Ramírez-Díaz, Martha I.; Riveros‐Rosas, Héctor; Cervantes, Carlos

doi:10.1007/s10534-011-9421-x

Cited by 177 publications

(136 citation statements)

References 126 publications

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“…In addition to these findings, there have been a number of incremental discoveries related to the binding of oxoanions64 which reveal interesting aspects of natural anion‐coordination strategies. The binding of nitrate by cyanobacteria provides nitrogen fixation for entire aqueous ecosytems.…”

Section: Macromolecular Recognition Of Anions In Watermentioning

confidence: 99%

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

2015

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The recognition of anions in water remains a key challenge in modern supramolecular chemistry, and is essential if proposed applications in biological, medical, and environmental arenas that typically require aqueous conditions are to be achieved. However, synthetic anion receptors that operate in water have, in general, been the exception rather than the norm to date. Nevertheless, a significant step change towards routinely conducting anion recognition in water has been achieved in the past few years, and this Review highlights these approaches, with particular focus on controlling and using the hydrophobic effect, as well as more exotic interactions such as C−H hydrogen bonding and halogen bonding. We also look beyond the field of small‐molecule recognition into the macromolecular domain, covering recent advances in anion recognition based on biomolecules, polymers, and nanoparticles.

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Section: Macromolecular Recognition Of Anions In Watermentioning

confidence: 99%

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

2015

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“…Surprisingly, the modABC genes had the highest w 700 nM MoϪ9 nM Mo values (3.4, 3.3, and 2.0, respectively) ( Table 1), suggesting that although growth was Mo limited, Mo uptake through ModABC was not essential under tungsten-inhibited conditions, in contrast to low-Mo conditions. Mo at micromolar to millimolar concentrations, however, can enter the cell via sulfate transporters or lowspecificity anion transporters (LSATs) as well as via ModABC (34). LSATs have long been suspected to exist, but the encoding genes have yet to be identified, with the exception of a low-specificity permease that transports MoO 4 2Ϫ , SO 4 2Ϫ , VO 4 3Ϫ , and WO 4 2Ϫ in Rhodobacter capsulatus (34,35).…”

Section: Resultsmentioning

confidence: 99%

“…Mo at micromolar to millimolar concentrations, however, can enter the cell via sulfate transporters or lowspecificity anion transporters (LSATs) as well as via ModABC (34). LSATs have long been suspected to exist, but the encoding genes have yet to be identified, with the exception of a low-specificity permease that transports MoO 4 2Ϫ , SO 4 2Ϫ , VO 4 3Ϫ , and WO 4 2Ϫ in Rhodobacter capsulatus (34,35). Our RB-TnSeq data for RCH2 now provide evidence for additional LSATs.…”

Section: Resultsmentioning

confidence: 99%

Determining Roles of Accessory Genes in Denitrification by Mutant Fitness Analyses

Vaccaro

Thorgersen

Lancaster

et al. 2016

Appl Environ Microbiol

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Enzymes of the denitrification pathway play an important role in the global nitrogen cycle, including release of nitrous oxide, an ozone-depleting greenhouse gas. In addition, nitric oxide reductase, maturation factors, and proteins associated with nitric oxide detoxification are used by pathogens to combat nitric oxide release by host immune systems. While the core reductases that catalyze the conversion of nitrate to dinitrogen are well understood at a mechanistic level, there are many peripheral proteins required for denitrification whose basic function is unclear. A bar-coded transposon DNA library from Pseudomonas stutzeri strain RCH2 was grown under denitrifying conditions, using nitrate or nitrite as an electron acceptor, and also under molybdenum limitation conditions, with nitrate as the electron acceptor. Analysis of sequencing results from these growths yielded gene fitness data for 3,307 of the 4,265 protein-encoding genes present in strain RCH2. The insights presented here contribute to our understanding of how peripheral proteins contribute to a fully functioning denitrification pathway. We propose a new low-affinity molybdate transporter, OatABC, and show that differential regulation is observed for two MoaA homologs involved in molybdenum cofactor biosynthesis. We also propose that NnrS may function as a membrane-bound NO sensor. The dominant HemN paralog involved in heme biosynthesis is identified, and a CheR homolog is proposed to function in nitrate chemotaxis. In addition, new insights are provided into nitrite reductase redundancy, nitric oxide reductase maturation, nitrous oxide reductase maturation, and regulation.

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“…Specifically, the H-RS cluster showed distinctive features for cofactor acquisition and/or biosynthesis ( Table 2; see also Table S5 in the supplemental material). Molybdate is being transported by a high-affinity system (modABC) that may also be able to transport sulfate (64), giving an extra molybdenum and sulfur source for H-RS bacteria. Vitamin B 6 , a cofactor synthesized by pyridoxamine 5=-phosphate oxidase (gene pdxH), is involved in many biological reactions in the central metabolism, but its concentration in the marine environments can range from undetectable to picomoles per liter (reference 65 and references therein).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Genomic Analyses of the OM43 Clade, Including a Novel Species from the Red Sea, Indicate Ecotype Differentiation among Marine Methylotrophs

Jimenez-Infante

Ngugi

Vinu

et al. 2016

Appl Environ Microbiol

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The OM43 clade within the family Methylophilaceae of Betaproteobacteria represents a group of methylotrophs that play important roles in the metabolism of C 1 compounds in marine environments and other aquatic environments around the globe. Using dilution-to-extinction cultivation techniques, we successfully isolated a novel species of this clade (here designated MBRS-H7) from the ultraoligotrophic open ocean waters of the central Red Sea. Phylogenomic analyses indicate that MBRS-H7 is a novel species that forms a distinct cluster together with isolate KB13 from Hawaii (Hawaii-Red Sea [H-RS] cluster) that is separate from the cluster represented by strain HTCC2181 (from the Oregon coast). Phylogenetic analyses using the robust 16S-23S internal transcribed spacer revealed a potential ecotype separation of the marine OM43 clade members, which was further confirmed by metagenomic fragment recruitment analyses that showed trends of higher abundance in low-chlorophyll and/or high-temperature provinces for the H-RS cluster but a preference for colder, highly productive waters for the HTCC2181 cluster. This potential environmentally driven niche differentiation is also reflected in the metabolic gene inventories, which in the case of the H-RS cluster include those conferring resistance to high levels of UV irradiation, temperature, and salinity. Interestingly, we also found different energy conservation modules between these OM43 subclades, namely, the existence of the NADH:quinone oxidoreductase complex I (NUO) system in the H-RS cluster and the nonhomologous NADH:quinone oxidoreductase (NQR) system in the HTCC2181 cluster, which might have implications for their overall energetic yields. Methylotrophs are a taxonomically diverse group of microorganisms that use reduced one-carbon (C 1 ) compounds as their sole carbon and energy sources (1, 2). Unlike methanotrophs, non-methane-utilizing methylotrophs (here simply denoted methylotrophs) cannot oxidize methane but instead degrade more oxidized C 1 compounds, like methanol, methylamine, and formaldehyde, using different pathways for their oxidation, demethylation, and assimilation into biomass (1-3). Marine methylotrophs play an important role in the metabolism and assimilation of C 1 compounds like methanol and methylated compounds containing amino, halide, and/or sulfur moieties in the oceans (4).Many model methylotrophs have been cultured, which has facilitated studies on their genomic and functional characteristics and diversity (3). Among the marine methylotrophs, the OM43 clade belonging to the Betaproteobacteria occurs abundantly in productive aquatic environments from coastal waters to brackish and freshwater ecosystems (5, 6). The occurrence of close relatives of the marine OM43 clade in freshwater habitats, which form a closely related, but separate, lineage known as the LD28 clade (5, 7), also implies that OM43-like organisms have a broader biogeographical distribution. Surveys in the western Atlantic determined that OM43 clade members represented 5% of the b...

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Bacterial transport of sulfate, molybdate, and related oxyanions

Cited by 177 publications

References 126 publications

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

Determining Roles of Accessory Genes in Denitrification by Mutant Fitness Analyses

Comprehensive Genomic Analyses of the OM43 Clade, Including a Novel Species from the Red Sea, Indicate Ecotype Differentiation among Marine Methylotrophs

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