Bacterial Spore Detection by [Tb³⁺(macrocycle)(dipicolinate)] Luminescence

Abstract

Experimental Section.Materials. DPA (dipicolinic acid) was purchased from Sigma-Aldrich and used as received.TbCl 3 was purchased from Alfa Aesar and used as received. DO2A (1,4,7,10-tetraazacyclododecane-1,7-diacetate) was obtained after hydrolysis (see reference 11) of DO2A-tBu-ester (1,4,7,10-tetraazacyclododecane-1,7-di(t-butyl acetate)), purchased from Macrocyclics.

Europium Complexes of Tris(dipicolinato) Derivatives Coupled to Methylumbelliferone: A Double Sensitization

“…[20,21] Near-infrared luminescence from Nd III , Dy III , Er III , Tm III , and Yb III is also sensitized by dipicolinate moieties, [9,22,23] although usually to a lesser extent than visible emission. For all these reasons, this family of complexes was used in several applications, such as various inorganic-organic hybrid materials, [24] nanoparticles, [25] or as analytical probes, particularly for the analysis of bacterial spores [26][27][28] or of nanomolar concentrations of Tb III . [29] Finally, lanthanide dipicolinates display nonlinear optical (NLO) properties [30,31] and two-photon excitation luminescence, either through an allowed Tb III f-f transition [32] or through the ligand, [33,34] enabling luminescence microscopy images to be collected under NIR excitation.…”

Surface Modification and Functionalization of Microporous Hybrid Material for Luminescence Sensing

“…[15,22] Further function of the new luminescent microporous hybrid materials with a silylated TTA-Tb led to 3, which can be used as luminescence probes for anthrax and other bacterial spores by complexing to DPA, which is a major constituent of many spore-forming bacteria. [16,23] Hybrid material 3 only shows europiumA C H T U N G T R E N N U N G (III) luminescence and no emission from terbiumA C H T U N G T R E N N U N G (III) is found when excited at 278 nm, because TTA could not sensitize the luminescence of terbiumA C H T U N G T R E N N U N G (III). The europiumA C H T U N G T R E N N U N G (III) complex from inside the nanochannel of zeolite L crystals encapsulated in the silica, as an internal calibration, is protected from the external influences such as solvents, providing a stable reference signals.…”

Lanthanide induced formation of novel luminescent alginate hydrogels and detection features

“…Ten percent of its dry weight of bacillus anthracis spores is calcium dipicolinate (CaDPA) (Scott & Ellar, 1978). The convenient, rapid and real-time detection of this unique biomarker has been explored (Cable, Kirby, Soarasaenee, Gray, & Ponce, 2007;Tan, Wang, & Zhang, 2011). Our team has already developed a carbon nanotube based terbium inorganic-organic network and it could recognize CaDPA by the modulation of the emissions .…”