Bacterial skin flora and contamination of blood components: do we defer blood donors wisely?

Pastila, Satu; Lönnroth, M.; Heikkilä, Riitta; Heikkilä, Heikki; Carlson, P.

doi:10.1111/j.1423-0410.2012.01591.x

Cited by 6 publications

(11 citation statements)

References 33 publications

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“…The association of high colonisation rates of S. aureus with atopic dermatitis is well known, and a recent study reported that among 74 eczema colonised patients, all but four harboured related S. aureus strains at three sampling sites [16]. In Finland, dermal disorders or a skin infection results in donor deferral as the number of bacteria at the VP site was shown to be significantly higher in individuals with such conditions compared with controls [17]. Two of the three apheresis donors implicated in our near-miss incidents reported nonhealing skin lesions on the leg and nose, respectively; the first donor was culture negative at the skin site but both donors had nasal carriage of S. aureus.…”

Section: Discussionmentioning

confidence: 99%

Failure of bacterial screening to detect Staphylococcus aureus: the English experience of donor follow‐up

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Failure of bacterial screening to detect Staphylococcus aureus: the English experience of donor follow‐up

et al. 2018

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“…Studies in Finland, Tanzania, and Morocco showed frequencies of S. aureus and Gramnegative bacteria between 7%-16.74% and 13.73%-41.2% of blood donors, respectively. 8,19,20 Of note, the Tanzanian study sampled skin from healthy participants (no blood donors) at the sterno-clavicular joint, which is categorized as sebaceous and a typical habitat for Gram-positive skin bacteria, whereas the antecubital fossa is categorized as moist and harbors Gram-negative bacteria. 16 In the Moroccan study, participants were voluntary blood donors, onethird of them trainees at the study hospital.…”

Section: Discussionmentioning

confidence: 99%

Presence of Gram‐negative bacteria and Staphylococcus aureus on the skin of blood donors in the Democratic Republic of the Congo

Heroes

Okitale

Ndalingosu³

et al. 2022

Transfusion

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Background Skin bacteria may contaminate blood products but few data are available on sub‐Saharan Africa (sSA). We assessed the presence of Gram‐negative bacteria and Staphylococcus aureus on blood donor skin and evaluated skin antisepsis in the Democratic Republic of the Congo (DRC). Study Design and Methods Among blood donors at the National Blood Transfusion Center (NBTC) and at a rural hospital, the antecubital fossa skin of the non‐disinfected arm (not used for blood collection) was swabbed (25cm2 surface) and cultured for total and Gram‐negative bacterial counts. Bacteria were identified with MALDI‐TOF and tested for antibiotic susceptibility by disk diffusion. For evaluation of the NBTC antisepsis procedure (i.e., ethanol 70%), the culture results of the disinfected arm (used for blood collection) were compared with those of the non‐disinfected arm. Results Median total bacterial counts on 161 studied non‐disinfected arms were 1065 Colony‐Forming Units (CFU) per 25 cm2, with 43.8% (70/160) of blood donors growing Gram‐negative bacteria and 3.8% (6/159) Staphylococcus aureus (2/6 methicillin‐resistant). Non‐fermentative Gram‐negative rods predominated (74/93 isolates, majority Pseudomonas spp., Acinetobacter spp.). Enterobacterales comprised 19/93 isolates (mostly Pantoea spp. and Enterobacter spp.), 5/19 were multidrug‐resistant. In only two cases (1.9%, 2/108) the NBTC antisepsis procedure met the acceptance criterion of ≤2 CFU/25 cm2. Conclusion Skin bacterial counts and species among blood donors in DRC were similar to previously studied Caucasian populations, including cold‐tolerating species and bacteria previously described in transfusion reactions. Prevention of contamination (e.g., antisepsis) needs further evaluation and customization to sSA.

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“…They were taken under non-sterile conditions, and it is possible that common contaminants like S . aureus , coagulase-negative staphylococci and Pseudomonas genera may have originated from the bacterial skin flora [8, 10].…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of microorganisms causing acute hand infections

et al. 2019

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Hand infections are a common presentation at the emergency departments. Without knowing the source of infection clinicians are dependent on systematic reports on the bacterial spectrum and susceptibility tests of the specific infection in their patient community. This study was based on a retrospective chart review of patients presenting to our outpatient clinic with acute hand infections. We documented patient demographics, the etiology, location, culture tests of the infection and analyzed if certain bacteria could be cultured significantly more often in certain etiologies or in specific sites of the hand infection. Susceptibility tests were added. Bacterial swabs of 204 patients were analyzed. Overall S . aureus was found in 53% of all cases, in only one case revealed methicillin-resistant S . aureus ( MRSA). There was no significant difference in the bacterial spectrum according to the etiology of the hand infections, except for animal bites where Pasteurella multocida was the dominating bacteria in 63% of all cases. Amoxicillin-clavulanic acid, fluoroquinolones, and piperacillin were effective against the main bacteria. Our study confirms the previously published antibiotic resistance reports and reinforces the current antibiotic treatment guidelines also in this western European population.

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Bacterial skin flora and contamination of blood components: do we defer blood donors wisely?

Cited by 6 publications

References 33 publications

Failure of bacterial screening to detect Staphylococcus aureus: the English experience of donor follow‐up

Failure of bacterial screening to detect Staphylococcus aureus: the English experience of donor follow‐up

Presence of Gram‐negative bacteria and Staphylococcus aureus on the skin of blood donors in the Democratic Republic of the Congo

Susceptibility of microorganisms causing acute hand infections

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