Bacterial resistance to antibiotics in acne vulgaris: An  in vitro  study

Hassanzadeh, Parichehr; Bahmani, Mahmoud; Mehrabani, Davood

doi:10.4103/0019-5154.43213

Cited by 43 publications

(34 citation statements)

References 14 publications

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“…Only 36.84% of the isolates were sensitive to tetracycline. Resistance to tetracycline in Staphylococcus aureus has been reported in the present study and in the study by Hassanzadeh et al [3] It may be due to extensive tetracycline usage in the past. It has been suggested that tetracycline, which becomes concentrated in inflamed lesions and has been the mainstay of acne treatment for two decades, could act by inhibiting neutrophil chemotaxis rather than its antibacterial actions.…”

Section: Discussionsupporting

confidence: 53%

“…This is in contrast to reports which implicated both Staphylococcus epidermidis and Propionibacterium acnes as bacteria causing acne vulgaris. [3] By using a genetically based strategy (Analysis of 16S rRNA genes) with sensitivity and discriminatory power surpassing those of culture-based methods, Bek-Thomsen et al demonstrated that the bacterial microbiota of follicles from acne-affected subjects showed more, although still very limited, diversity. The microbiota was dominated by Propionibacterium acnes and Staphylococcus epidermidis, which were the only species consistently found.…”

Section: Discussionmentioning

confidence: 99%

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Bacteriology of Acne

Srikanth¹,

Kalyani²,

Mohan³

et al. 2015

jemds

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CONTEXT: Acne vulgaris is the most common skin disorder in youth and is of multifactorial etiology. Severe forms of acne are often therapy resistant. AIMS: To determine bacteria involved in acne vulgaris and to determine the in vitro antibiotic sensitivity of aerobic isolates. SETTINGS AND DESIGN: Cross sectional study. METHODS AND MATERIALS: Samples from skin lesions of study group (80 patients) and normal skin of control group (20 patients) were collected by sterile swabs. The specimens were used for aerobic and anaerobic culture at 37°C. The isolates were subjected to biochemical tests for identification. All the aerobic isolates were subjected to antimicrobial sensitivity testing by disc diffusion method. RESULTS: High incidence of acne was found in the age group of 13-20 years (76.25%). Males were more affected than females. Pustules (76.25%) were the commonest presentation. Students (71.25%) were more commonly affected. Among aerobic isolates (71.24%), Staphylococcus epidermidis (54.38%) was the most common organism. Among the anaerobes, Propionibacterium acnes (55.17) were the most common. Among the aerobic isolates, most were sensitive to Minocycline followed by Ofloxacin, Azithromycin, Cephalexin, Tetracycline, Cotrimoxazole and Ampicillin. CONCLUSIONS: In the present study, acne affected individuals were young males, especially students, with pustules being the commonest presentation. Staphylococcus epidermidis, the dominant organism isolated was susceptible to Ofloxacin, Minocycline and Azithromycin.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Bacteriology of Acne

Srikanth¹,

Kalyani²,

Mohan³

et al. 2015

jemds

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“…TCS is used as an antibacterial agent in dermal products against acne vulgaris, which is thought to be caused primarily by S. aureus (10,32). Mean zone of inhibition (mm±SD) could be listed in order as: The microbiological studies revealed that the formulations were effective, and this antimicrobial effect was significantly greater than TCS suspension in water and commercial product but the same for both organogels (Fig.…”

Section: Triclosan In Mw Gelmentioning

confidence: 99%

A Novel Preparation Method for Organogels: High-Speed Homogenization and Micro-irradiation

et al. 2013

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Abstract. The aim of this work was to prepare organogels of Carbopol 974P NF (C974) in PEG 400 by using a novel technique, high-speed homogenization followed by microwave heating. Triclosan (TCS) was used as a model drug. C974, at concentrations ranging between 2% and 4%, was dispersed in 25 ml of PEG 400, and the dispersion was homogenised for 5 min at 24,000 rpm. The dispersion was either heated at 80°C in water bath under mechanic stirring at 200 rpm or exposed to micro-irradiation (1,200 W/1 h) for 2 min. The formulations prepared with both methods performed a well-structured gel matrix characteristic at 3% and 4% of C974 concentrations. As the concentrations of the polymer increased, the elastic properties also increased. The viscosity profiles indicated a shear-thinning system. DSC data revealed that TCS was dissolved in gel. Skin accumulation ability of TCS had been improved by these novel organogels regardless of the preparation method. TCS was still microbiologically effective after the microwave process was applied. It was determined that microwave heating is a suitable method to obtain C974 organogels. This novel production technique developed might be promising especially in industrial scale when the dramatic reduction in the preparation time and energy were considered.

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“…Ayrıca stafilokokların da akne vulgariste olası patogenetik rolü olduğuna dair önemli in vitro bulgular vardır. Yapılan bir çalışmada akne açısından değerlendirilen katılımcıların %53'ünün Staphylococcus epidermidis ile kolonize olduğu gösterilmiştir 2 . Kırk yılı aşkın süredir akne tedavisinde kullanılan antibiyotikler, hala sıklıkla bu amaçla reçete edilmektedir.…”

Section: Introductionunclassified

Resistance status of antibiotics in Gram-positive bacteria isolated from acne lesions in İstanbul

Doğan¹,

Bektöre²,

Karabacak³

et al. 2017

TURKDERM

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Background and Design: Acne vulgaris is a multifactorial disease in which Propionibacterium acnes is thought to play an important role in the pathogenesis of inflamed lesions. There is also significant in vitro evidence suggesting a possible pathogenetic role for Staphylococci in acne vulgaris and an in vitro study of patients who were undergoing evaluation for acne, showed that 53% of participants were colonised with Staphylococcus epidermidis. Materials and Methods: In this study, 29 P. acnes isolates and 61 coagulase-negative staphylococci (CNS) isolates were obtained from 169 specimens belonging to patients with inflammatory acne vulgaris and the antimicrobial susceptibilities of these isolates against nadifloxacin, erythromycin, clindamycin and tetracycline were determined at the minimum inhibitory concentration levels, using the 'Clinical and Laboratory Standards Institute' agar dilution method. Results: There were no P. acnes isolate resistant to the antibiotics tested in this study and the antibiotic resistance status among CNS was as 28%, 36%, 23% and 0% for tetracycline, erythromycin, clindamycine and nadifloxacin, respectively. Conclusion: According to the results of this study, it may be expected that nadifloxacin could be more effective in the treatment of acne than other antimicrobial agents because of having no resistance to both P. acnes and CNS. However, this must be supported by clinical trials. Although resistance rates were found to be relatively low in this study, the antibiotic resistance problem should be taken into consideration while planning a treatment to get successful results. In case of treatment failures, presence of drug resistant strains should be considered. Keywords: Antibiotic resistance, topical, acne, İstanbul Amaç: Akne vulgaris, inflame lezyonların patogenezinde Propionibacterium acnes'in önemli bir rol oynadığı düşünülen multifaktöriyel bir hastalıktır. Ayrıca stafilokokların da akne vulgariste olası patogenetik rolü olduğuna dair önemli in vitro bulgular vardır. Yapılan bir çalışmada, akne açısından değerlendirilen katılımcıların %53'ünün Staphylococcus epidermidis ile kolonize olduğu gösterilmiştir. Gereç ve Yöntem: Bu çalışmada enflamatuvar akneli hastalardan sağlanan 169 örnekten, 29 P. acnes ve 61 koagülaz negatif stafilokok (KNS) izolatı elde edilmiş ve bu izolatların nadifloksasin, eritromisin, klindamisin ve tetrasikline karşı antimikrobiyal duyarlılıkları Klinik ve Laboratuvar Standartları Kurumu 'Clinical and Laboratory Standards Institute' agar dilüsyon metodu kullanılarak minimum inhibisyon konsantrasyonu seviyesinde belirlenmiştir. Bulgular: Hiçbir P. acnes izolatı bu çalışmada kullanılan antibiyotiklere dirençli değildi. KNS izolatlardaki direnç durumu ise tetrasiklin, eritromisin, klindamisin ve nadifloksasine karşı sırasıyla %28, %36, %23 ve %0 olarak saptandı. Sonuç: Bu çalışma verilerine göre, nadifloksasine karşı P. acnes ve KNS izolatlarında herhangi bir direnç saptanmaması nedeniyle, nadifloksasinin akne tedavisinde daha etkili olabileceği düşünülebil...

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Bacterial resistance to antibiotics in acne vulgaris: An in vitro study

Cited by 43 publications

References 14 publications

Bacteriology of Acne

Bacteriology of Acne

A Novel Preparation Method for Organogels: High-Speed Homogenization and Micro-irradiation

Resistance status of antibiotics in Gram-positive bacteria isolated from acne lesions in İstanbul

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