Bacterial pyrite oxidation: release of iron and scanning electron microscopic observations

Hiltunen, Paula; Vuorinen, Antti; Rehtijärvi, Pentti; Tuovinen, Olli H.

doi:10.1016/0304-386x(81)90019-0

Cited by 26 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There have been many direct observations of the attachment of acidophilic, chemolithotrophic bacteria, T. ferrooxidans and Sulfolobus sp., to sulfide minerals (10)(11)(12)(13)(14)(15)(16). By using the scanning electron microscope, it has been shown that T. ferrooxidans is attached to the surfaces of sulfide minerals such as pyrite, chalcopyrite, and galena and that the surfaces of sulfide particles exposed to T. ferrooxidans are eroded characteristically to show bacterially etched pits (13)(14)(15)(16).…”

Section: Bacterial Growth and Iron Dissolution In M9k Pyrite Mediummentioning

confidence: 99%

“…By using the scanning electron microscope, it has been shown that T. ferrooxidans is attached to the surfaces of sulfide minerals such as pyrite, chalcopyrite, and galena and that the surfaces of sulfide particles exposed to T. ferrooxidans are eroded characteristically to show bacterially etched pits (13)(14)(15)(16). In the present study, direct observation showed that T. ferrooxidans was attached to some restricted areas of pyrite ore surfaces and that the surfaces were eroded intensively in characteristic patterns similar to those described previously (13)(14)(15)(16), but without prominent attachment of bacterial cells on the eroded surfaces during the enhanced leaching. The small area covered by the cells seems to indicate that the pyrite ore surfaces are highly heterogeneous with respect to their affinity for the bacteria.…”

Section: Bacterial Growth and Iron Dissolution In M9k Pyrite Mediummentioning

confidence: 99%

See 1 more Smart Citation

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

Wakao

Mishina

Sakurai

et al. 1984

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

The cells of 'Thiobacillus ferrooxidans were rapidly adsorbed on the solid surfaces of an agitated flask containing 1 % pulp density of pyrite particles. More than ca. 99 % of the inoculated cells were adsorbed. However, considerably fewer cells were adsorbed on pyrite particles than on the glass wall of the flask. Scanning electron microscope observation revealed that T. ferrooxidans cells were adsorbed aggregatively on restricted areas of the pyrite particles. The surfaces of the pyrite particles were characteristically eroded to show etched polyhedral pits, but without prominent cell adsorption of the extensively eroded surfaces during markedly enhanced leaching. When T. ferrooxidans cells were adsorbed on the solid surfaces, the iron-oxidizing activity of the bacteria was strongly inhibited, resulting in a failure to enhance pyrite oxidation. Adsorbed cells did not proliferate on the solid surfaces. When the adsorbed cells were released into an aqueous phase by the addition of the surface active agent Tween 20, the bacterial iron-oxidizing activity inhibited by adsorption was recovered and, as a result, pyrite oxidation was markedly promoted. Significant enhancement of pyrite oxidation by T, ferrooxidans was ascribed to the development and iron-oxidizing activity of the freely dispersed cells in an aqueous phase. The function of the surface active agent is to prevent tenacious adsorption of the bacterial cells to solid surfaces and organic substances examined, such as protein, nucleic acid, yeast extract, peptone, and cell free extracts, operate in the same way as the surface active agent. The enhancement of the bacterial pyrite oxidation by the intact cells of Thiobacillus thiooxidans is thought to be attributable to the organic substances excreted from T. thiooxidans cells and/or to the exchange adsorption of cells of both thiobacilli. The present results indicate that bacterial concentration 63

show abstract

Section: Bacterial Growth and Iron Dissolution In M9k Pyrite Mediummentioning

confidence: 99%

Section: Bacterial Growth and Iron Dissolution In M9k Pyrite Mediummentioning

confidence: 99%

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

Wakao

Mishina

Sakurai

et al. 1984

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In the range 23-35 of 2, a broad peak identifying scorodite (FeAsO 4 ) is also observed. 18) Scorodite may form according to the following set of reactions in aerobic conditions:…”

Section: Resultsmentioning

confidence: 99%

FE-SEM Study of Microbially Formed Jarosites by <I>Acidithiobacillus ferrooxidans</I>

et al. 2006

View full text Add to dashboard Cite

Morphological characterization of jarosite groups formed from Fe(III) biologically oxidized with different numbers of Acidithiobacillus ferrooxidans was conducted using FE-SEM. The higher population of A. ferrooxidans resulted in more distinct jarosite mineral shape, and stronger Raman intensities for potassium jarosite, ammoniojarosite and argentojarosite. The morphology of the jarosites might be dependent on iron-oxidizing activity of A. ferrooxidans.The technique was applied to identify jarosite compounds formed during microbially mediated dissolution of arsenopyrite by A. ferrooxidans. It is difficult to identify this jarosite compound by X-ray diffraction and Raman spectroscopy because amounts are typically low and the crystallization is poor in minerals formed by microbially mediated oxidation. However, FE-SEM image provided helpful information for identification of jarosite compounds.The results suggest that morphology would provide useful information for identification and history of jarosite minerals as geochemical samples.

show abstract

“…The cultures were incubated at 21 to 23°C for 20 days. and complexing of the elements by organic compounds (Kee and Bloomfield, 1961;Schnitzerand Kodama, 1977;Jackson and Skippen, 1978;Albertsen et al, 1980;Hiltunen et al, 1981). Variations in the amounts of dissolved elements are mainly due to the differences of the surface area of the rock samples, properties of the surface, including the orientation of the mineral species, and the relative proportions of light colored and mafic minerals at the surface Holdren and Berner, 1979).…”

Section: Resultsmentioning

confidence: 99%

Bacterial weathering of rapakivi granite

Vuorinen

Mantere-Alhonen

Uusinoka

et al. 1981

Geomicrobiology Journal

Self Cite

View full text Add to dashboard Cite

Rapakivi granite samples were incubated with Pseudomonas aeruginosa culture solutions in order to elucidate the possible role of bacteria in rapakivi (crumbling stone) disintegration. SEM micrographs showed micromorphological alterations on the incubated rapakivi surface at 21 to 23°C for 20 days. Elevated concentrations of Na, Ca, K, Fe, and Mg were detected in the culture solutions after incubation. Elemental oxide ratios [K 2 O : (Na 2 O + CaO)] in culture solutions were similar to those in rapakivi ovoids, suggesting a proportional dissolution pattern of these elements.

show abstract

Bacterial pyrite oxidation: release of iron and scanning electron microscopic observations

Cited by 26 publications

References 20 publications

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

FE-SEM Study of Microbially Formed Jarosites by <I>Acidithiobacillus ferrooxidans</I>

Bacterial weathering of rapakivi granite

Contact Info

Product

Resources

About