Bacterial profile and antimicrobial susceptibility pattern in septicemia suspected patients attending Gondar University Hospital, Northwest Ethiopia

Abstract

BackgroundBacterial blood stream infection constitutes a significant public health problem and it is an important cause of morbidity and mortality in hospitalized patients. The aim of this study was to assess the prevalence of bacterial isolates from septicemia suspected patients and their antimicrobial susceptibility pattern in Gondar University Hospital.MethodsThis laboratory based retrospective study of 390 blood culture and susceptibility tests was conducted in Bacteriology Laboratory of the University of Gondar Teaching Hospital. The samples were collected and processed following standard microbiological techniques as part of the routine clinical management of the patient. Antibiotic susceptibility testing was done on pure culture isolates employing disc-diffusion method for the commonly used antibiotics. The data were analyzed by using SPSS version 16 and the results were summarized by using tables and graphs.ResultsOut of 390 blood culture results, 71 (18.2%) were culture positive. The predominant bacteria isolated from blood culture were Coagulase negative staphylococci 30 (42.3%), followed by S. aureus 17 (23.9%) and Klebiesella spp 9 (12.9%), E. coli 5 (7.0%), Pseudomonas aeroginosa 4 (5.6%) and Salmonella spp. 3 (4.2%). The gram positive and gram negative bacteria constituted 49 (69%) and 22 (31%) of the culture isolates; respectively. The isolates showed high rates of resistance to most antibiotics tested. The range of resistance for Gram positive and Gram negative were from 23.5% – 58.8%, and 20%– 100% respectively.ConclusionsIn the present study most of the pathogens isolated from blood culture showed high rate of resistance to most commonly used antibiotics used to treat bacterial infections. Therefore, rational use of antibiotics should be practiced.

Bacteriological Profile and Antibiotic Sensitivity Pattern of Hospitalacquired Septicemia in a Tertiary Care Hospital in North East India

“…CoNS was mainly recognized as mere contaminants till 1970's; however, several studies have now reported an increasing incidence of infection by this group of bacteria [19,20]. The isolation of CoNS is consistent with the study of Arora et al [11] and Karlowsky et al [20] where the reported isolation of the organism was 42% and 21%, respectively.…”

Diagnosis and Treatment of Typhoid Fever and Associated Prevailing Drug Resistance in Northern Ethiopia

“…Overall the range of drug resistance pattern for gram positives was from 0% -89.7% and from 0% -100% for gram negative bacteria, which is similar to the result from other part of Ethiopia, which was 0-85.7% and 0% -100% for gram negative and positive respectively. 20 This increased resistance in this study may be an indication of indiscriminate and continuous use of antibiotics as clearly seen in our study, where more patients (66.7%) were put on the wrong treatments. Thirty six (87.7%) of the S. aureus isolates were resistant to Trimethoprim-sulphamethoxazole, 34(82.9%) to ceftriazone 31(75.6%) to doxycycline, 51% to erythromycin, 39% to Gentamicin.…”

“…21,23,24 . CoNS were mainly recognized as a contaminant until the 1970's, nevertheless, several studies have reported an increasing incidence of infections due to these bacteria 25,26 . This was similar to our current study.…”

Resistance profiles and biofilm formation of coagulase negative staphylococci isolated from clinical specimens in a tertiary care hospital in Palestine

“…Irrespective of geographical locations, a worldwide SENTRY study showed that, about 70-75% of CoNS are resistant to methicillin [10]. There is a significant increase in the methicillin-resistant coagulase negative staphylococci (MR-CoNS) infections and these bacteria have recently started to gain resistance to other widely used antibiotics [11][12][13][14].The increased recognition of pathogenic potential of CoNS and emergence of drug resistance among them justify the need to identify various species of CoNS and determine their antibiotic resistance pattern. Epidemiological data about CoNS in Palestine are either scarce or insufficient [15].…”