Bacterial phospholipases C with dual activity: phosphatidylcholinesterase and sphingomyelinase

Monturiol-Gross, Laura; Villalta-Romero, Fabián; Flores-Dı́az, Marietta; Alape-Girón, Alberto

doi:10.1002/2211-5463.13320

Cited by 11 publications

(12 citation statements)

References 124 publications

(212 reference statements)

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“…[ 46 ] suggest its crucial role in downregulating host immunity [ 68 ]. In L. monocytogenes , plc aided bacterial escape toward the cytosol and cell-to-cell propagation, whereas, in C. perfringens, it helped bacteria induce endothelial damage and platelet aggregation, and in P. aeruginosa , it led to the triggering of signaling pathways that lead to inflammation [ 69 ].…”

Section: Virulence Factorsmentioning

confidence: 99%

Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

2022

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Elizabethkingia spp. is a ubiquitous pathogenic bacterium that has been identified as the causal agent for a variety of conditions such as meningitis, pneumonia, necrotizing fasciitis, endophthalmitis, and sepsis and is emerging as a global threat including in Southeast Asia. Elizabethkingia infections tend to be associated with high mortality rates (18.2–41%) and are mostly observed in neonates and immunocompromised patients. Difficulties in precisely identifying Elizabethkingia at the species level by traditional methods have hampered our understanding of this genus in human infections. In Southeast Asian countries, hospital outbreaks have usually been ascribed to E. meningoseptica, whereas in Singapore, E. anophelis was reported as the main Elizabethkingia spp. associated with hospital settings. Misidentification of Elizabethkingia spp. could, however, underestimate the number of cases attributed to the bacterium, as precise identification requires tools such as MALDI-TOF MS, and particularly whole-genome sequencing, which are not available in most hospital laboratories. Elizabethkingia spp. has an unusual antibiotic resistance pattern for a Gram-negative bacterium with a limited number of horizontal gene transfers, which suggests an intrinsic origin for its multidrug resistance. Efforts to prevent and further understand Elizabethkingia spp. infections and limit its spread must rise to this new challenge.

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Section: Virulence Factorsmentioning

confidence: 99%

Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

2022

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“…Both the hemolytic ( plC H ) and the non-hemolytic phospholipase C ( plC N ) play an important role in the pathogenesis of P. aeruginosa infections. The products of these bacterial enzymes compete with the second messengers of the host enzymes, affecting physiological cell processes and disrupting cell membrane lipid homeostasis [ 21 ]. There is a noticeable difference in the number of publications on phospholipase genes, with the hemolytic phospholipase described much more frequently.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Virulence-Factor-Encoding Genes and Genotype Distribution amongst Clinical Pseudomonas aeruginosa Strains

Bogiel

Depka

Kruszewski

et al. 2023

IJMS

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Pseudomonas aeruginosa is an opportunistic pathogen encoding several virulence factors in its genome, which is well-known for its ability to cause severe and life-threatening infections, particularly among cystic fibrosis patients. The organism is also a major cause of nosocomial infections, mainly affecting patients with immune deficiencies and burn wounds, ventilator-assisted patients, and patients affected by other malignancies. The extensively reported emergence of multidrug-resistant (MDR) P. aeruginosa strains poses additional challenges to the management of infections. The aim of this study was to compare the incidence rates of selected virulence-factor-encoding genes and the genotype distribution amongst clinical multidrug-sensitive (MDS) and MDR P. aeruginosa strains. The study involved 74 MDS and 57 MDR P. aeruginosa strains and the following virulence-factor-encoding genes: lasB, plC H, plC N, exoU, nan1, pilA, and pilB. The genotype distribution, with respect to the antimicrobial susceptibility profiles of the strains, was also analyzed. The lasB and plC N genes were present amongst several P. aeruginosa strains, including all the MDR P. aeruginosa, suggesting that their presence might be used as a marker for diagnostic purposes. A wide variety of genotype distributions were observed among the investigated isolates, with the MDS and MDR strains exhibiting, respectively, 18 and 9 distinct profiles. A higher prevalence of genes determining the virulence factors in the MDR strains was observed in this study, but more research is needed on the prevalence and expression levels of these genes in additional MDR strains.

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“…Enterohemolysin EhxA and phospholipase C were found to be present, and both of these proteins are essential for evading the tissue system of the host. 115 , 171 - 174 …”

Section: Discussionmentioning

confidence: 99%

“…Enterohemolysin EhxA and phospholipase C were found to be present, and both of these proteins are essential for evading the tissue system of the host. 115,[171][172][173][174] Since virulence factors facilitate the colonization of the pathogen at the cellular level of the host and cause disease by evading the host defense mechanisms, it is necessary to have an understanding of the biological function and mechanism of virulence factors to comprehend the role they play during the pathogenesis of bacteria. 175,176 Also, in the case of bacterial infections, virulent factors (Supplementary Table 8) have the potential to be used as drug target.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Hypothetical Proteins of Vibrio parahaemolyticus Reveals the Presence of Virulence Factors and Growth-Related Enzymes With Therapeutic Potential

Shahrear

Zinnia

Sany

et al. 2022

Bioinform Biol Insights

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Vibrio parahaemolyticus, an aquatic pathogen, is a major concern in the shrimp aquaculture industry. Several strains of this pathogen are responsible for causing acute hepatopancreatic necrosis disease as well as other serious illness, both of which result in severe economic losses. The genome sequence of two pathogenic strains of V. parahaemolyticus, MSR16 and MSR17, isolated from Bangladesh, have been reported to gain a better understanding of their diversity and virulence. However, the prevalence of hypothetical proteins (HPs) makes it challenging to obtain a comprehensive understanding of the pathogenesis of V. parahaemolyticus. The aim of the present study is to provide a functional annotation of the HPs to elucidate their role in pathogenesis employing several in silico tools. The exploration of protein domains and families, similarity searches against proteins with known function, gene ontology enrichment, along with protein-protein interaction analysis of the HPs led to the functional assignment with a high level of confidence for 656 proteins out of a pool of 2631 proteins. The in silico approach used in this study was important for accurately assigning function to HPs and inferring interactions with proteins with previously described functions. The HPs with function predicted were categorized into various groups such as enzymes involved in small-compound biosynthesis pathway, iron binding proteins, antibiotics resistance proteins, and other proteins. Several proteins with potential druggability were identified among them. In addition, the HPs were investigated in search of virulent factors, which led to the identification of proteins that have the potential to be exploited as vaccine candidate. The findings of the study will be effective in gaining a better understanding of the molecular mechanisms of bacterial pathogenesis. They may also provide an insight into the process of evaluating promising targets for the development of drugs and vaccines against V. parahaemolyticus.

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Bacterial phospholipases C with dual activity: phosphatidylcholinesterase and sphingomyelinase

Cited by 11 publications

References 124 publications

Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

Comparison of Virulence-Factor-Encoding Genes and Genotype Distribution amongst Clinical Pseudomonas aeruginosa Strains

Functional Analysis of Hypothetical Proteins of Vibrio parahaemolyticus Reveals the Presence of Virulence Factors and Growth-Related Enzymes With Therapeutic Potential

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