Bacterial metabolism of carbofuran

Chaudhry, G. Rasul; Ali, Asmaa

doi:10.1128/aem.54.6.1414-1419.1988

Cited by 100 publications

(52 citation statements)

References 21 publications

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“…Pseudomonas sp. 50432 [22] was maintained and grown in Luria-Bertani medium with less than usual NaCl (1 g of NaCl, 10 g of tryptophan and 5 g of yeast extract, pH 7.0) or minimum medium (MM) containing 4. 8 3 .…”

Section: Organism and Culture Conditionsmentioning

confidence: 99%

Purification and biochemical characterization of the carbamate hydrolase from Pseudomonas sp. 50432

Chaudhry

Mateen

Kaskar

et al. 2002

Biotech and App Biochem

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A soluble carbamate hydrolase that had a wide specificity was purified 2032-fold from Pseudomonas sp. 50432. This was achieved using a combination of anion-exchange, gel-filtration and hydrophobic-interaction- chromatography techniques. Carbamate hydrolase cleaved the ester linkage of the N-methylcarbamates. The native enzyme was a monomer with a molecular mass of 88 kDa. The optimum pH and temperature of the enzyme activity were 8.5 and 37 degrees C respectively. The tested cations or EDTA did not affect the enzyme activity. However, 2-mercaptoethanol reversibly inhibited the enzyme activity. The enzyme showed the K(m) values of 16 and 12 microM for carbofuran and carbaryl respectively. The purified enzyme did not hydrolyse o-nitrophenyl dimethylcarbamate but hydrolysed several N-methylcarbamates and 1-naphthyl acetate.

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Section: Organism and Culture Conditionsmentioning

confidence: 99%

Purification and biochemical characterization of the carbamate hydrolase from Pseudomonas sp. 50432

Chaudhry

Mateen

Kaskar

et al. 2002

Biotech and App Biochem

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“…It is now generally accepted that adapted soil microorganisms are responsible for the rapid degradation of soilapplied pesticides following repeated treatments with the same or similar compounds (Racke and Coats 1990). Several authors have reported the isolation and characterization of carbofuran-degrading fungi (Williams et al 1976), actinomycetes (Williams et al 1976 ;Venkateswarlu andSethunathan 1984, 1985) and bacteria belonging to several different genera (Felsot et al 1981;Rajagopal et al 1984;Karns et al 1986;Chaudry and Ali 1988;Ramanand et al 1988;Head et al 1992;Parekh et al 1992). Other workers have used several different enrichment techniques and have usually succeeded in isolating only one or two types of carbofuran-degrading bacteria from any one soil.…”

Section: Carbofuranmentioning

confidence: 99%

Carbofuran‐degrading bacteria from previously treated field soils

Parekh¹,

Suett²,

Roberts³

et al. 1994

Journal of Applied Bacteriology

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Laboratory incubation studies were made on soils collected from five field sites with different histories of treatment with carbofuran. All soils treated earlier with carbofuran degraded the compound more rapidly than untreated samples of the same soils. Reduced rates of degradation in the presence of chloramphenicol imply that soil bacteria are primarily responsible for the breakdown of carbofuran in these soils. Sixty-eight bacteria, capable of degrading carbofuran as the sole source of carbon and nitrogen, were isolated from liquid cultures of treated soils. The concentration of carbofuran in the liquid medium used for isolation and subsequent culture of carbofuran-degrading isolates appeared to affect the stability of their ability to degrade. Similar types of carbofuran-degrading bacteria were isolated from different soils and several different types were isolated from one soil. All carbofuran-degrading isolates were Gram-negative, aerobic rods which hydrolysed the insecticide to carbofuran phenol. They were separated into four groups on the basis of a limited number of phenotypic characters. There was a good correlation between the phenotype of carbofuran-degrading isolates and the stability of their ability to degrade. Fourteen isolates were placed in phenotypic group I and 13 of these did not degrade carbofuran after one subculture in liquid medium. Phenotypic groups II, III and IV consisted of 54 isolates in total (3, 46 and 5 isolates respectively) and 52 of these retained their ability to degrade carbofuran when subcultured.

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“…It is also degraded in soil and water by hydrolysis, photolysis and photodecomposition, yielding 2-hydroxyfuradan and furadan phenol [9]. Furthermore, the hydrolysis of carbamate esters, carbamate side chains and carbofuran phenol has also been reported [6,10,11]. Interestingly, our earlier studies on pesticide degraders have suggested their innate capability of simultaneous expression of ancillary traits along with the pesticide biodegradation potential [12,13].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel carbofuran degradingPseudomonassp. with collateral biocontrol and plant growth promoting potential

Bano¹,

Musarrat²

2004

FEMS Microbiology Letters

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The isolate NJ-101 obtained from agricultural soil was characterized and presumptively identified as Pseudomonas sp. The isolate exhibited efficient degradation of the insecticide carbofuran with a rate constant of 0.035 day 31 , following first-order rate kinetics. The ability of performing multifarious biological activities in tandem suggested the uniqueness of isolate NJ-101. The ability to produce hydrogen cyanide and siderophore stipulated its role in biological control. Furthermore, the growth inhibition of Fusarium sp. validated the antagonistic activity of NJ-101 against the common phytopathogens. Concurrent production of indole acetic acid, and solubilization of inorganic phosphate revealed its plant growth promoting potential. Thus, the innate capability of this novel isolate for parallel biodegradation, biocontrol and plant growth promotion has significance in management of the agro-environmental and phytopathological problems.

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Bacterial metabolism of carbofuran

Cited by 100 publications

References 21 publications

Purification and biochemical characterization of the carbamate hydrolase from Pseudomonas sp. 50432

Purification and biochemical characterization of the carbamate hydrolase from Pseudomonas sp. 50432

Carbofuran‐degrading bacteria from previously treated field soils

Characterization of a novel carbofuran degradingPseudomonassp. with collateral biocontrol and plant growth promoting potential

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