Bacterial Lysis of Fibrin Seal in vitro

Hoffmann, Steen; Moesgaard, F

doi:10.1159/000128744

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Slow fibrin clot lysis by S. pneumoniae was identified by one study. The results were linked to possibly low plasminogen concentration [46]. Thus, our results can be explained in the same manner and fibrinolysis of S. pneumoniae may take longer than three hours to occur.…”

Section: Discussionsupporting

confidence: 60%

Comparative Analysis of Blood Clot, Plasma Rich in Growth Factors and Platelet-Rich Fibrin Resistance to Bacteria-Induced Fibrinolysis

et al. 2019

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Alveolar osteitis (AO) is a common, painful postoperative complication after tooth extraction. Fibrinolytic activity in the extraction socket is one etiological factor. Platelet concentrates are used to prevent and treat AO. The aim of this study was to find out whether the positive effect of platelet concentrates can be related to resistance to bacteria-induced fibrinolysis. Blood from 45 human volunteers was used to prepare four media: blood clot medium as control group; PRF and PRGF first fraction (PRGF I) and PRGF second fraction (PRGF II) as study groups. Additionally, collected blood was used for blood plasma preparation on which evaluation of initial value of d-dimer concentration was performed. A solution of five different microbes (Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumonia, Bacillus cereus, and Candida albicans) was adjusted to 0.5 McFarland (1 × 108 CFU/mL) and then diluted to 0.25 McFarland (0.5 × 108 CFU/mL). The d-dimer concentration was evaluated after one and three hours of bacteria exposure. The resistance to fibrinolysis was not statistically distinguished among any media groups at any time. S. pneumoniae was statistically active in PRF after three hours. C. albicans was statistically active in PRGF II after one hour and in PRF between the first and third hour and after three hours. S. aureus and B. cereus were statistically active in PRGF II after three hours. S. pyogenes was statistically active after one hour, between the first and third hour, and after the third hour in all groups. S. pyogenes was the most active bacterium. Different blood formulations were not distinguishable based on resistance to bacteria-induced fibrinolysis. Low fibrinolytic properties of the found major microbes suggests that bacteria-induced fibrinolysis is one of the leading causes of absence of a clot in a post-extraction socket to be clinically insignificant. The initial absence of a clot or its mechanical elimination during formation or the healing period are major causes of dry socket.

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Section: Discussionsupporting

confidence: 60%

Comparative Analysis of Blood Clot, Plasma Rich in Growth Factors and Platelet-Rich Fibrin Resistance to Bacteria-Induced Fibrinolysis

et al. 2019

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“…This has been used both to eradicate perineal sepsis, which is associated with recurrence in the conventional treatment of fistulae [38], and because certain bacterial species seem capable of lysing fibrin seal in vitro [39]. Antibiotics have been administered either pre‐operatively, intra‐operatively and/or postoperatively, and either parenterally [15,20,22,24], enterally [18,21], locally to the fistula tract [17,19], mixed with the fibrin glue [14,19], or simply not given [11,13,23].…”

Section: Antibiotic Therapymentioning

confidence: 99%

Fibrin glue in the management of anal fistulae

2004

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“…This has been used both to eradicate perineal sepsis, which is associated with recurrence in the conventional treatment of fistulae [7] and because certain bacterial species seem capable of lysing fibrin seal in vitro [8] . Antibiotics have been administered either pre-operatively, intra-operatively and/or post-operatively, and either parenteraly, enterally, locally to the fistula tract.…”

Section: Pre-operative Antibioticmentioning

confidence: 99%

Role of Fibrin Glue in Management of Perianal Fistula in Assiut University Hospital

Mostafa¹,

Aziz

Ali³

et al. 2018

The Medical Journal of Cairo University

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Background: A fistula-in-ano is an abnormal hollow tract or cavity that is lined with granulation tissue and that connects a primary opening inside the anal canal to a secondary opening in the perianal skin; secondary tracts may be multiple and can extend from the same primary opening. Patients and Methods:In our study we had 40 patients who were complainingof fistula in ano (high and low perianal fistula). They were evaluated in the post-operative period for the complications as recurence, sphincteric disturbance, postoperative abscess and post-operative pain from Results: After injection of 40 cases of perianal fistula it was found that 5 cases out of 16 cases of high perianal fistula (31.2%) developed recurrence of their fistulas tract, while 11cases did not develop recurrence (69.8%) after 6 moths follow-up. On the other hand 5 cases of low perianal fistula out of 24 cases (20.8%) developed recurrence of their fistulas with 19 cases (79.2%) that did not develop recurrence their fistulous tract after 6 months follow-up. Other complication noticed in this study was post-operative abscess and reported in 3 cases only (7.5%). There was no post-operative incontinence or post-operative pain or bleeding reported in this study.Conclusion: Injection of fibrin glue in aperianal fistula is simple, easy and safe technique with lower rate of complication as loss of sphincteric function, post-operative pain, intra operative bleeding and post-operative wound infection. This technique can be repeated even at the out patient clinic.

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Bacterial Lysis of Fibrin Seal in vitro

Cited by 5 publications

References 9 publications

Comparative Analysis of Blood Clot, Plasma Rich in Growth Factors and Platelet-Rich Fibrin Resistance to Bacteria-Induced Fibrinolysis

Comparative Analysis of Blood Clot, Plasma Rich in Growth Factors and Platelet-Rich Fibrin Resistance to Bacteria-Induced Fibrinolysis

Fibrin glue in the management of anal fistulae

Role of Fibrin Glue in Management of Perianal Fistula in Assiut University Hospital

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