Bacterial imaging with photostable upconversion fluorescent nanoparticles

Ong, Li Ching; Ang, Lei; Alonso, Sylvie; Zhang, Yong

doi:10.1016/j.biomaterials.2013.12.060

Cited by 65 publications

(48 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zhang and co-workers 84 have taken advantage of the high photostability of antibody-based bacterial targeting NaYF 4 :Yb,Er NPs to uniformly label and long term monitor E. coli in infected dendritic cells in vitro. The same strategy can also be used to reach a much more difficult target such as glioblastoma in brain.…”

Section: Chem Soc Revmentioning

confidence: 99%

Imaging agents based on lanthanide doped nanoparticles

et al. 2015

View full text Add to dashboard Cite

Nanotechnology has recently allowed us to design and prepare nanoplatforms with the potential to face currently unresolved problems. Among these platforms, nanoparticles in particular are versatile objects that find applications in many different areas. In the vast ensemble of materials that have been explored to obtain nanoparticles with improved performances, we here focus our attention on lanthanide-based nanocrystals. These recently developed species are extremely interesting and well known particularly for their ability to emit anti-Stokes shifted light (upconversion) with relatively high brightness. Many advantageous characteristics of such materials are emerging, and their use as multimodal imaging agents is rapidly growing. We here survey some recent examples on this subject, mainly focusing on systems having NIR-to-NIR emission properties for in vivo applications.

show abstract

Section: Chem Soc Revmentioning

confidence: 99%

Imaging agents based on lanthanide doped nanoparticles

et al. 2015

View full text Add to dashboard Cite

show abstract

“…1,2 There are several potential benefits for the use of nanocrystals with UPC emission in biological applications, such as no damage of tissues; anti-Stokes emission; long lifetimes; photostability; increased contrast in biological specimens due to the absence of autofluorescence upon excitation with IR light; and simultaneous detection of multiple targeted analytes. [3][4][5][6] Other advantages of the UPC emission are the reduction of photobleaching and scattering in tissues, which avoid the use of complicated and high-cost femtosecond lasers and photomultiplier tubes. [7][8][9][10] For biomedical applications, such as cancer detection, biolabeling, and bioimaging, luminescent nanoparticles preferably have to form a stable colloidal solution under physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

et al. 2015

View full text Add to dashboard Cite

Abstract. This work reports the synthesis, structural characterization, and optical properties of ZrO 2 ∶Yb 3þ -Er 3þ (2-1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. The conjugation was successfully confirmed by Fourier transform infrared, zeta potential, and dynamic light scattering. The internalization of the conjugated nanoparticles in human cervical cancer (HeLa) cells was followed by two-photon confocal microscopy. The ZrO 2 ∶Yb 3þ -Er 3þ nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, the luminescence change due to the addition of APTES molecules and biomolecules on the nanocrystals was also studied. These results demonstrate that ZrO 2 ∶Yb 3þ -Er 3þ nanocrystals can be successfully functionalized with biomolecules to develop platforms for biolabeling and bioimaging. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

“…Thereafter, the PR‐linker containing PEG and ONA was successfully synthesized and conjugated on the surface of UCNPs@SiO 2 –COOH modified substrate by ester bond formation. The fabricated quartz substrate was defined as an UCNPs@SiO 2 ‐PR linker substrate, and the detailed conjugation process is illustrated in Figure (UCNP‐based substrate proposed in this study can be produced on a large scale, and then under preservation for a long time due to the high stability of UCNPs . Herein, the dynamical control substrate fabricated in this study does not need to be used immediately after preparation.…”

Section: Resultsmentioning

confidence: 99%

“…UCNPs have significant properties such as their attractive optical, low autofluorescence and the fact that a light‐scattering background can be induced by biological samples . Additionally, UCNPs have excellent photostability, which potentially enables the long‐term dynamical control of cells without any significant signal intensity losses . These properties are particularly useful for the dynamic control of pathogenic bacteria .…”

Section: Introductionmentioning

confidence: 99%

Fabricating upconversion fluorescent nanoparticles modified substrate for dynamical control of cancer cells and pathogenic bacteria

Chen

Zhao

et al. 2016

J. Biophotonics

View full text Add to dashboard Cite

Lanthanide-doped upconversion nanoparticles (UCNPs) have attracted widespread interests in the field of biomedicine because of their unique upconverting capability by converting near infrared (NIR) excitation to visible or ultraviolet (UV) emission. Here, we developed a novel UCNP-based substrate for dynamic capture and release of cancer cells and pathogenic bacteria under NIR-control. The UCNPs harvest NIR light and convert it to ultraviolet light, which subsequently result in the cleavage of photoresponsive linker (PR linker) from the substrate, and on demand allows the release of a captured cell. The results show that after seeding cells for 5 h, the cells were efficiently captured on the surface of the substrate and ˜89.4% of the originally captured S. aureus was released from the surface after exposure to 2 W/cm NIR light for 30 min, and ˜92.1% of HepG2 cells. These findings provide a unique platform for exploring an entirely new application field for this promising luminescent nanomaterial.

show abstract

Bacterial imaging with photostable upconversion fluorescent nanoparticles

Cited by 65 publications

References 34 publications

Imaging agents based on lanthanide doped nanoparticles

Imaging agents based on lanthanide doped nanoparticles

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

Fabricating upconversion fluorescent nanoparticles modified substrate for dynamical control of cancer cells and pathogenic bacteria

Contact Info

Product

Resources

About