Bacterial ghosts as a novel advanced targeting system for drug and DNA delivery

Paukner, Susanne; Stiedl, Thomas; Kudela, Pavol; Bízik, Jozef; Laham, Firas Al; Lubitz, Werner

doi:10.1517/17425247.3.1.11

Cited by 53 publications

(27 citation statements)

References 85 publications

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“…It has already been described how Gram-negative bacteria can be used for constructing bacterial ghosts, representing novel advanced delivery and targeting vehicles suitable for the delivery of hydrophobic or water-soluble drugs. 2,21 Also, in pathogenic fungi, like C. albicans, several components associated with the cell wall have been identified to play an important role in fungal-host interactions. 22 We have previously demonstrated that by simple mixing of nanoparticles of gold and platinum with Salmonella enteritidis and Listeria monocytogenes, a process of selfassembly takes place, but with different morphologic interactions depending on the bacteria and nanoparticles used.…”

Section: Introductionmentioning

confidence: 99%

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

Chwalibog¹,

Sawosz²,

Hotowy³

et al. 2010

IJN

257

149

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Purpose:The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism-nanoparticle vehicles. Methods: Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Results: Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a "noncontact" interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Conclusion: Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.

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Section: Introductionmentioning

confidence: 99%

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

Chwalibog¹,

Sawosz²,

Hotowy³

et al. 2010

IJN

257

149

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“…The use of BGs as candidate vaccines and advanced drug carriers can be found in several recent reviews. 24,27,29,[32][33][34][35][36] …”

Section: Introductionmentioning

confidence: 99%

The bacterial ghost platform system

Langemann¹,

et al. 2010

Self Cite

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The Bacterial Ghost (BG) platform technology is an innovative system for vaccine, drug or active substance delivery and for technical applications in white biotechnology. BGs are cell envelopes derived from Gram-negative bacteria. BGs are devoid of all cytoplasmic content but have a preserved cellular morphology including all cell surface structures. Using BGs as delivery vehicles for subunit or DNA-vaccines the particle structure and surface properties of BGs are targeting the carrier itself to primary antigenpresenting cells. Furthermore, BGs exhibit intrinsic adjuvant properties and trigger an enhanced humoral and cellular immune response to the target antigen. Multiple antigens of the native BG envelope and recombinant protein or DNA antigens can be combined in a single type of BG. Antigens can be presented on the inner or outer membrane of the BG as well as in the periplasm that is sealed during BG formation. Drugs or supplements can also be loaded to the internal lumen or periplasmic space of the carrier. BGs are produced by batch fermentation with subsequent product recovery and purification via tangential flow filtration. For safety reasons all residual bacterial DNA is inactivated during the BG production process by the use of staphylococcal nuclease A and/or the treatment with β-propiolactone. After purification BGs can be stored long-term at ambient room temperature as lyophilized product. The production cycle from the inoculation of the pre-culture to the purified BG concentrate ready for lyophilization does not take longer than a day and thus meets modern criteria of rapid vaccine production rather than keeping large stocks of vaccines. The broad spectrum of possible applications in combination with the comparably low production costs make the BG platform technology a safe and sophisticated product for the targeted delivery of vaccines and active agents as well as carrier of immobilized enzymes for applications in white biotechnology.

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“…To this end, the thermoinducible phage fX174 gene E was used for autolysis of bacterial vector. This lysin gene functions to cause bacterial inner and outer membrane to fuse, leading to the formation of a transmembrane tunnel through which the cytoplasmic content is expelled (Paukner et al, 2006). Consequently, bacteria stopped growing upon the induced production of fX174 gene E (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The former was created by chain shuffling (Schier et al, 1996), while the latter was derived from IgG-binding domain of staphylococcal protein A and was extensively screened from a mutant library (Orlova et al, 2006). Moreover, a programmed lysis system was constructed to liberate the plasmid cargo in E. coli by controlled production of phage fX174 gene E (Paukner et al, 2006). By these approaches, the engineered bacterial strain was shown to mediate the expression of green fluorescent protein (GFP) in HER2/neu-positive tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Engineering of Escherichia coli for targeted delivery of transgenes to HER2/neu‐positive tumor cells

Chang

Cheng

Chen

et al. 2011

Biotech & Bioengineering

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Targeting of non-phagocytic tumor cells and prompt release of gene cargos upon entry into tumors are two limiting steps in the bacterial gene delivery path. To tackle these problems, the non-pathogenic Escherichia coli strain BL21(DE3) was engineered to display the anti-HER2/neu affibody on the surface. After co-incubation with tumor cells for 3 h, the anti-HER2/neu affibody-presenting E. coli strain was selectively internalized into HER2/neu-positive SKBR-3 cells. The invasion efficiency reached as high as 30%. Furthermore, the bacteria were equipped with the phage ϕX174 lysin gene E-mediated autolysis system. Carrying the transgene (e.g., eukaryotic green fluorescent protein, GFP), the tumor-targeting bacteria were subjected to the thermal shock to trigger the autolysis system upon entry into HER2/neu-positive cells. Flow cytometric analysis revealed that 3% of infected cells expressed GFP 24 h post thermal induction. Overall, the results show a promise of the proposed approach for developing bacteria as a delivery carrier.

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Bacterial ghosts as a novel advanced targeting system for drug and DNA delivery

Cited by 53 publications

References 85 publications

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

The bacterial ghost platform system

Engineering of Escherichia coli for targeted delivery of transgenes to HER2/neu‐positive tumor cells

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