Engineering of <i>Escherichia coli</i> for targeted delivery of transgenes to HER2/<i>neu</i>‐positive tumor cells

Chang, Chih‐Hsiang; Cheng, Wei-Jou; Chen, Shan-Yu; Kao, Ming‐Ching; Chiang, Chung-Jen; Chao, Yun-Peng

doi:10.1002/bit.23095

Cited by 19 publications

(9 citation statements)

References 36 publications

(50 reference statements)

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“…In contrast, nonviral vectors are safe, have low immunogenicity, and are relatively inexpensive [4]. Examples of nonviral vectors include bacteria [5], cell-penetrating peptides [6], functionalized gold nanoparticles (NPs) or carbon nanotubes [7-10], and cationic polymers. Among these nonviral vectors, cationic polymers including polyethyleneimine (PEI) [11], poly(l-lysine) (PLL) [11, 12], chitosan [13], dendrimers [14, 15] and cationic lipids [16] have the advantages of being scalable for manufacturing in quantity, having low immunogenicity, the capacity for selective chemical modification and the ability to carry large inserts.…”

Section: Introductionmentioning

confidence: 99%

Dual-purpose magnetic micelles for MRI and gene delivery

Wang

Sheshala

Martinez

et al. 2012

Journal of Controlled Release

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Gene therapy is a promising therapeutic approach for treating disease, but the efficient delivery of genes to desired locations with minimal side effects remains a challenge. In addition to gene therapy, it is also highly desirable to provide sensitive imaging information in patients for disease diagnosis, screening and post-therapy monitoring. Here, we report on the development of dual-purpose chitosan and polyethyleneimine (PEI) coated magnetic micelles (CP-mag-micelles) that can deliver nucleic acid-based therapeutic agents and also provide magnetic resonance imaging (MRI). These ‘theranostic’ CP-mag-micelles are composed of monodisperse hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) loaded into the cores of micelles that are self-assembled from a block copolymer of poly (D, L-lactide) (PLA) and monomethoxy polyethylene glycol (mPEG). For efficient loading and protection of the nucleic acids the micelles were coated with cationic polymers, such as chitosan and PEI. The morphology and size distribution of the CP-mag-micelles were characterized and their potential for use as an MRI-probe was tested using an MRI scanner. The T2 relaxivity of micelles was similar to CP-mag-micelles confirming that coating with cationic polymers did not alter magnetism. Nanoparticles coated with chitosan:PEI at a weight ratio of 5:5 showed higher transfection efficiency in HEK293, 3T3 and PC3 cells than with weight ratios of 3:7 or 7:3. CP-mag-micelles are biocompatible, can be delivered to various organs and are safe. A single injection of CP-mag-micelles carrying reporter plasmids in vivo expressed genes for at least one week. Collectively, our results demonstrate that a structural reinforcement of SPIONs loaded in the core of an mPEG-PLA micelle coated with cationic polymers provides efficient DNA delivery and enhanced MRI potential, and affords a promising candidate for theranostics in the future.

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Section: Introductionmentioning

confidence: 99%

Dual-purpose magnetic micelles for MRI and gene delivery

Wang

Sheshala

Martinez

et al. 2012

Journal of Controlled Release

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“…However, the mechanism of the antibiotic action on lysis remains to be elucidated. In addition to antibiotic treatment, intracellular lysis of the E. coli bactofection vector may be improved by expression of phage lysins (Chang et al, 2011), or through the manipulation of concentration of the compound for which the vector is auxotrophic (Simon et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Induction of Protein Expression Within Escherichia coli Vector for Entry into Mammalian Cells

Chen

Lee

Sim

et al. 2014

Human Gene Therapy Methods

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Direct protein delivery into the cytosol of mammalian cells by invasive Escherichia coli (E. coli) bacterial vector will bypass the need to achieve nuclear entry and transcription of DNA, a major hurdle that is known to seriously limit gene transfer. The bacterial vector is induced to express the protein during its growth phase, before presentation for entry into mammalian cells and release of its content into the cellular environment. For this class of vector, crossing the plasma membrane becomes the primary step that determines the success of protein delivery. Yet, how the mechanics of protein expression within the vector affect its entry into the host is poorly understood. We found the vector's effectiveness to enter HeLa cells diminished together with its viability when phage N15 protelomerase (TelN) expression was induced continuously in the invasive E. coli despite producing an abundant amount of functional protein. By comparison, shorter induction, even as little as 3 hr, produced sufficient amounts of functional TelN and showed more effective invasion of HeLa cells, comparable to that of uninduced invasive E. coli. These results demonstrate that brief induction of protein expression during vector growth is essential for optimal entry into mammalian cells, an important step for achieving bacteria-mediated protein delivery.

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“…In this regard, targeted delivery of a therapeutic gene is considered as an important possibility due to its ability to target a specific receptor on the cancer cell surface with subsequent therapeutics delivery. A gene‐delivery system derived from microbes has also been applied for targeted gene therapy of a nonphagocytic cancer cell; the engineered bacterial strain ( Escherichia coli ) has been shown to mediate the expression of a transgene in a specified tumor cell . Furthermore, targeted gene delivery is considered an effective strategy to treat drug‐resistant cancer cells .…”

Section: Introductionmentioning

confidence: 99%

Development of a ZHER3‐Affibody‐Targeted Nano‐Vector for Gene Delivery to HER3‐Overexpressed Breast Cancer Cells

Nazari

Koukhaloo

Mousavi

et al. 2019

Macromolecular Bioscience

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Despite the initial successes of gene delivery applications, they faced on several intrinsic drawbacks including toxicity and immunogenicity. Therefore, alternative gene‐delivery systems derived from recombinant peptides have emerged and is rapidly developing. Human epidermal growth factor receptor‐3 (HER3) shows high activity in tumor resistance to anti‐human epidermal growth factor receptor 2 (HER2) therapies. In this study, an affibody molecule against HER3 is conjugated to a biomimetic peptide RALA (an amphipathic and cationic peptide enriched with arginine) and the ability of the fusion vector for targeting HER3 and afterward delivering specific genes in breast cancer cells is evaluated. The results demonstrate that the biopolymeric platform, which contains an affibody‐conjugated RALA peptide, can effectively condense DNA into nanoparticles and target the overexpressed HER3 receptors in breast cancer cells and transfer specific genes. The use of such a recombinant biopolymer may pave the way for the development of sensitive and effective diagnostic and treatment tool for breast cancer.

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Engineering of Escherichia coli for targeted delivery of transgenes to HER2/neu‐positive tumor cells

Cited by 19 publications

References 36 publications

Dual-purpose magnetic micelles for MRI and gene delivery

Dual-purpose magnetic micelles for MRI and gene delivery

Induction of Protein Expression Within Escherichia coli Vector for Entry into Mammalian Cells

Development of a ZHER3‐Affibody‐Targeted Nano‐Vector for Gene Delivery to HER3‐Overexpressed Breast Cancer Cells

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