Bacterial exopolysaccharides produced by newly discovered bacteria belonging to the genus Halomonas , isolated from hypersaline habitats in Morocco

Bouchotroch, S; Quesada, Emilia; Izquierdo, Isidoro; Rodríguez, Miguel Delgado; Béjar, Victoria

doi:10.1038/sj.jim.7000002

Cited by 56 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the other hand, they presented great phenotypic similarity amongst themselves, as was revealed by numerical analysis of their phenotypic attributes (Bouchotroch et al, 1999). These strains produced EPS of considerable biotechnological interest (Bouchotroch et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…This bacterium produces an exopolysaccharide, named EPS S-31, which is of potential industrial use as a viscosifier and emulsifier. The physical and chemical properties of this polymer are described elsewhere (Bouchotroch et al, 2000). Briefly, it is composed of the neutral sugars glucose, mannose and galactose at a ratio of 1 : 4 : 2n5.…”

Section: Discussionmentioning

confidence: 99%

“…Although the market for polymers already has recourse to gums of remarkable quality, the diversity of micro-organisms being discovered offers excellent prospects of finding new EPS with new or better properties. The polymers found in our laboratory, for example, possess advantageous qualities not shared by many polysaccharides currently in use : they have simultaneous emulsifying and viscosifying activities ; some are able to gel at acidic pH ; they are quite resistant to high osmotic strength ; and are also thermostable (Bouchotroch et al, 2000 ;Calvo et al, 1998). We are carrying out a wide search aimed at isolating and Recently, the results of a taxonomic study of a group of 46 moderately halophilic, EPS-producing bacteria isolated from hypersaline environments in Morocco were reported.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium.

Bouchotroch¹,

Quesada²,

Moral³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

Self Cite

157

130

View full text Add to dashboard Cite

Four moderately halophilic, exopolysaccharide-producing bacterial strains isolated from soil samples collected from a saltern at Asilah (Morocco) are reported. These four strains were initially considered to belong to the genus Halomonas. Their DNA GMC contents varied between 622 and 641 mol %. DNA-DNA hybridization revealed a considerable degree of DNA-DNA similarity amongst all four strains (755-808 %). Nevertheless, similarity with the reference strains of phylogenetically close relatives was lower than 40 %. 16S rRNA gene sequences were compared with those of other species of Halomonas and other Gram-negative bacteria and they were sufficiently distinct phylogenetically from other recognized Halomonas species to warrant their designation as a novel species. The name Halomonas maura sp. nov. is therefore proposed, with strain S-31 T (l CECT 5298 T l DSM 13445 T ) as the type strain. The fatty acid composition of strain S-31 T revealed the presence of 18 :1ω7c, 16:1ω7c/2-OH i15 :0 and 16 :0 as the major components. Growth rate analysis showed that strain S-31 T had specific cationic requirements for Na M and Mg 2M .

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium.

Bouchotroch¹,

Quesada²,

Moral³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

Self Cite

157

130

View full text Add to dashboard Cite

show abstract

“…EPS produced by H. maura, H. almeriensis, H. xianhensis and Salipiger mucosus were able to emulsify hydrocarbons, crude oils, mineral oils, hexadecane, tetradecane, octane and many others. 63,92 Moreover, the highest anionic activity of EPS produced by Halomonas sp. TG39 was correlated with 100% emulsifying capacity of hexadecane.…”

Section: Halophilic Eps: Properties and Applicationsmentioning

confidence: 99%

Untitled

2017

BIJ

View full text Add to dashboard Cite

Halophilic microorganisms derived from diverse thalassohaline and athalassohaline environments including marine estuaries, saline and soda lakes, inland solar salterns and acidic habitats are categorized as slight-, moderate-and extreme halophiles according to their NaCl requirements. Taxonomic studies with culturable diversities of halophiles revealed that they belong to both Archaea and Bacteria representing the families Halobacteriaceae, Methanosarcinaceae and the class Gammaproteobacteria. As adaptive strategies against harsh salt stresses, majority of halophiles often synthesize and accumulate extracellular polysaccharides (EPS) which differ significantly in terms of their physical, chemical and material properties. So far the novelty in structure and functions of exopolysaccharides are concerned, producer strains belonging to the genera Halomonas and Haloferax have attracted the main attention. However, EPS producing strains belonging to the genera Idiomarina, Salipiger and Alteromonas are not uncommon. Through process optimization and metabolic regulation a number of potent halophilic strains have been found to produce copious amount of EPS indicating its commercial viability. Moreover, the significance of production, physico-chemical and biological properties along with the possible applications of halophilic EPS in industry and biotechnology have also been highlighted..

show abstract

“…Further, the fermentation conditions play important role in determining the conformation, molecular mass, monosaccharide ratios and functional properties of the EPS (Sutherland, 1994;Finore et al, 2014). But until recently, there have been very few studies on the effect of nutrients and cultural conditions on the production and composition of EPS of moderately halophilic bacteria (Bouchotroch et al, 2000;Arias et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Cultural Conditions for Production of Exopolysaccaride by <i>Halomonas marina</i> HMA 103 under Batch-Culture

Pal¹,

Biswas²,

Chatterjee³

et al. 2015

American Journal of Microbiology

View full text Add to dashboard Cite

A moderately halophilic bacterium, Halomonas marina HMA 103 (MTCC 8968) accumulating intracellular polyesters also produced significant amount of exopolysaccharides (EPS) under batch-culture. Production of EPS by the bacterium was more or less parallel with the growth in modified basal synthetic medium. During growth, it produced 0.46 g/L of EPS after 50 h of incubation and was accompanied by the complete utilization of glucose from the medium. EPS production was maximum in 2% (w/v) glucose followed by sucrose and maltose and was positively influenced by yeast extract, beef extract and peptone. Optimum concentrations of sulfate and phosphate for EPS production were 2.4% (w/v) and 0.03% (w/v) respectively. High concentration (10% w/v) of NaCl stimulated both growth and EPS production by H. marina HMA 103. Characteristically, this EPS is a heteropolymer of glucose and mannose and the Fourier Transform Infrared (FTIR) spectrum showed distinct absorption peaks at 3424.2, 1638.7, 1122.9 and 622.3 cm −1 indicating the presence of free hydroxyl, phenyl or carbonyl groups of carbohydrate. Further, the detailed physico-chemical analysis will indicate the significance and potential importance of this EPS in biotechnology.

show abstract

Bacterial exopolysaccharides produced by newly discovered bacteria belonging to the genus Halomonas , isolated from hypersaline habitats in Morocco

Cited by 56 publications

References 0 publications

Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium.

Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium.

Untitled

Optimization of Cultural Conditions for Production of Exopolysaccaride by <i>Halomonas marina</i> HMA 103 under Batch-Culture

Contact Info

Product

Resources

About