Bacterial Degradation of Fungicide Captan

Megadi, Veena B.; Tallur, Preeti N.; Mulla, Sikandar I.; Ninnekar, Harichandra Z.

doi:10.1021/jf1030339

Cited by 31 publications

(16 citation statements)

References 33 publications

(29 reference statements)

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“…Captan has been shown to augment the soil N status by reducing the microbial communities responsible for capturing N and by providing digestible carbon that can be immobilized into the bacterial biomass (Martínez-Toledo et al 1998;Megadi et al 2010a;Megadi et al 2010b). It is possible that the 56 kg ha −1 N release from the cereal rye at Kinston when compared to Beltsville during the first month of the study (Fig.…”

Section: Discussionmentioning

confidence: 99%

In situ validation of fungal N translocation to cereal rye mulches under no-till soybean production

et al. 2016

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Background and aims The ability of grass mulches to inhibit weed performance has been linked to their limitations on nitrogen availability to the weeds. Fungal translocation of N from the soil to the surface mulch has been confirmed in laboratories, but this mechanism has not been documented under field conditions. Methods Experiments used 15 N (NH 4 ) 2 SO 4 , 99.7 at.%, which was uniformly injected below the soil surface at a rate of 1 mg 15 N kg −1 soil. Some plots were treated with a fungicide (Captan) every 2 weeks after injection, while others were not treated. Nitrogen transfer was monitored by measuring levels in surface residue, soybean tissue, and extractable soil inorganic N pools.Results Despite the N release from the cereal rye (Secale cereale L.) tissues ranging from 15 to 50 kg N ha, there was a detectable increase in 15 N enrichment of 10-15 % in the cereal rye tissue. Six weeks after injection, tissue from the plots not treated with fungicide contained 36 % more 15 N. ConclusionsThe increased 15 N enrichment in the cereal rye mulch supports laboratory observations that soil inorganic N is translocated into surface mulch via fungal mechanisms. These findings illustrate microbialmediated sinks for nitrogen in cereal rye mulches in no-till soybean production systems.

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Section: Discussionmentioning

confidence: 99%

In situ validation of fungal N translocation to cereal rye mulches under no-till soybean production

et al. 2016

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“…Although several reports on the degradation of triazoles in soil are available, microbial degradation studies have been vastly under represented. A few reports on tubaconazole and other fungicide degradation by bacteria isolated from contaminated soil have also been reported (Nicole et al 2009;Megadi et al 2010;Elhussein et al 2011). Moreover, Pseudomonas fluorescence was found to degrade 10 % to 70 % tubeconazole in the culture medium with a time gap of 6-21 days.…”

Section: Discussionmentioning

confidence: 90%

Biodegradation of the fungicide propiconazole by Pseudomonas aeruginosa PS-4 strain isolated from a paddy soil

Satapute

Kaliwal

2016

Ann Microbiol

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In India, propiconazole, a triazole group fungicide, is broadly used against powdery mildew, rusts, and leaf spot diseases of cereals and coffee. The toxicity of this fungicide is known to affect the quality of the soil. Hence, in the present study, a bacterium isolated from contaminated paddy soil was used to study the degradation of propiconazole under in vitro conditions. The isolated bacterium was confirmed as Pseudomonas aeruginosa strain (PS-4) based on morphological and biochemical characteristics, and 16S rRNA gene sequencing. When the isolated bacterium was grown in mineral salt medium amended with 10 μg/l propiconazole as a sole carbon source, culture filtrates of the bacterium utilized up to 8 μg/L of propiconazole after 72 h of incubation at 30°C and pH 7, as analyzed by HPLC. Degradation of propiconazole by the bacterium was also aided by the secretion of three metabolites-1,2,4-triazole; 2,4-dichlorobenzoic acid; and 1-chlorobenzene-as determined by their mass spectra. Furthermore, induction of monooxygenase activity and the CYP450 gene was observed in the culture filtrate of strain PS-4, showing evidence of their role in the degradation of propiconazole. These results revealed that PS-4 is an efficient candidate for the reduction of contaminants present in the soil, thereby contributing to soil health and crop improvement.

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“…We have previously reported the pendimethalin reduction by pendimetha lin nitroreductase into two steps, viz. reduction to 6 aminopendimethalin, and oxidative dealkylation to 3,4 dimethyl 2,6 dinitroaniline [15]. There are few reports on the purification and characterization of nitroreductases from microorganisms [5,8,11,12] but no information is available for the purification and characterization of pendimethalin nitroreductase from bacteria and fungi.…”

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confidence: 99%

Purification and properties of pendimethalin nitroreductase from Bacillus circulans

More¹,

Tallur²,

Ninnekar

et al. 2015

Appl Biochem Microbiol

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A pendimethalin nitroreductase was purified from Bacillus circulans to 52 fold increase with a recovery of 21.1% and had a specific activity of 4.16 U/mg. The molecular weight was 27 kDa. The optimum pH and temperature were 7.5 and 35°C, respectively. Fe 2+ , Ca 2+ , Mg 2+ and Mn 2+ did not cause any effect on the enzyme activity, whereas Ag + , Hg 2+ significantly inhibited it. The pendimethalin nitroreductase from B. circulans required NADPH as cofactor. It was not affected by GSH, DTT, L Cys and β mercaptoethanol but inhibited by p hydroxymercuribenzoate, N ethylmaleimide, and iodoacetamide. EDTA, 1,10 phenan throline and α,α' dipyridyl had a moderate effect on the enzyme activity. The nitroreductase reduced all the tested nitroaromatic compounds but was more active towards pendimethalin and trifluralin. The K M and V max for the enzyme reaction using pendimethalin as a substrate were 4.35 μM and 620 μmoles mg -1 min -1 , respectively. The broad substrate specificity towards dinitroaniline herbicides suggested the enzyme to be used as a potent reducing agent of these toxic compounds. This is the first report for the purification and charac terization of pendimethalin nitroreductase from any bacterial or fungal species.

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Bacterial Degradation of Fungicide Captan

Cited by 31 publications

References 33 publications

In situ validation of fungal N translocation to cereal rye mulches under no-till soybean production

In situ validation of fungal N translocation to cereal rye mulches under no-till soybean production

Biodegradation of the fungicide propiconazole by Pseudomonas aeruginosa PS-4 strain isolated from a paddy soil

Purification and properties of pendimethalin nitroreductase from Bacillus circulans

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