Bacterial culture of apheresis platelets: a mathematical model of the residual rate of contamination based on unconfirmed positive results

Benjamin, Richard J.; Dy, Beth A.; Pérez, Jaime; Eder, Anne F.; Wagner, Stephen J.

doi:10.1111/vox.12065

Cited by 52 publications

(89 citation statements)

References 20 publications

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“…Despite the availability of bacterial detection methods, bacterial contamination of PCs still poses the highest risk of transfusion‐transmitted disease . Bacteria can be missed in early testing due to low bacterial concentrations.…”

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confidence: 99%

Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

et al. 2018

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BACKGROUND The THERAFLEX UV‐Platelets system (Maco Pharma) uses ultraviolet C (UVC) light for pathogen inactivation (PI) of platelet concentrates (PCs) without any additional photoactive compound. The aim of the study was to systematically investigate bacterial inactivation with this system under conditions of intended use. STUDY DESIGN AND METHODS The robustness of the system was evaluated by assessing its capacity to inactivate high concentrations of different bacterial species in accordance with World Health Organization guidelines. The optimal use of the PI system was explored in time‐to‐treatment experiments by testing its ability to sterilize PCs contaminated with low levels of bacteria on the day of manufacture (target concentration, 100 colony‐forming units/unit). The bacteria panel used for spiking experiments in this study included the World Health Organization International Repository Platelet Transfusion Relevant Reference Strains (n = 14), commercially available strains (n = 13), and in‐house clinical isolates (n = 2). RESULTS Mean log reduction factors after UVC treatment ranged from 3.1 to 7.5 and varied between different strains of the same species. All PCs (n = 12/species) spiked with up to 200 colony‐forming units/bag remained sterile until the end of storage when UVC treated 6 hours after spiking. UVC treatment 8 hours after spiking resulted in single breakthrough contaminations with the fast‐growing species Escherichia coli and Streptococcus pyogenes. CONCLUSION The UVC‐based THERAFLEX UV‐Platelets system efficiently inactivates transfusion‐relevant bacterial species in PCs. The comprehensive data from this study may provide a valuable basis for the optimal use of this UVC‐based PI system.

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confidence: 99%

Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

et al. 2018

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“…The probability of bacterial detection with primary culture is a function of the input volume, timing of testing relative to collection, the size of the bacterial inoculum, and the kinetics of bacterial growth for the implicated organism itself. The latter can be slow growing, thus escaping early detection at the blood collection center . By increasing the time relative to collection, secondary bacterial culture optimizes the probability of detection.…”

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confidence: 99%

Implementation of secondary bacterial culture testing of platelets to mitigate residual risk of septic transfusion reactions

et al. 2018

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“…(3 cases) [5]. Benjamin et al [14] examined bacterial culture of platelet products free from antibodies and found the same bacterial species that are associated with sepsis [14]. …”

Section: Discussionmentioning

confidence: 99%

Bacterial contamination of platelet products in the Blood Transfusion Center of Isfahan, Iran

Farzad

Baghban

Bamzadeh

et al. 2016

GMS Hygiene and Infection Control; 11:Doc23

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Bacterial culture of apheresis platelets: a mathematical model of the residual rate of contamination based on unconfirmed positive results

Cited by 52 publications

References 20 publications

Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

Implementation of secondary bacterial culture testing of platelets to mitigate residual risk of septic transfusion reactions

Bacterial contamination of platelet products in the Blood Transfusion Center of Isfahan, Iran

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