Bacterial Contamination of the Feeding Bag during Catheter Jejunostomy: Exogenous or Endogenous Origin?

Leeuw, I. De; Alsenoy, Lauren Van; Delvigne, C

doi:10.1177/0148607184008005591

Cited by 8 publications

(6 citation statements)

References 1 publication

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“…When incubated at room temperature, the number of GBS per ml increased from 2.1 X 10' to 3.2 x 106 after 8 hr (Table I). Replication continued, reaching 6.9 x 107 per ml by 24 hr. At 37°C, the number of GBS per ml increased from 1.8 x 106 to 5.5 x 10' over 8 hr.…”

Section: Resultsmentioning

confidence: 96%

“…Retrograde introduction of intestinal tract organisms into the feeding tube and upper respiratory tract bacterial contamination of the tube during insertion have been described. 22,24 To minimize the risks associated with proliferation of endogenous contaminants in the solutions, it is recommended that solutions and equipment be frequently changed in accordance with the current guidelines.…”

Section: Discussionmentioning

confidence: 99%

“…A volume of sterile or GBS-contaminated Osmolite was then transferred to glass feeding bottles which were placed in the animal cages and replaced daily. The volume of ENS remaining in the bottles after 24 hr was measured and the amount ingested per mouse was calculated. The mean daily intake of ENS per mouse was 40 ml, with daily variations ranging from 35 to 45 ml.…”

Section: Osmolitementioning

confidence: 99%

“…One flask was incubated at room temperature (20-24°C) and the other at 37°C. Quantitative analyses of samples were made at 0, 4, 8, and 24 hr. The 1.0-ml sample was serially diluted in sterile saline and 0.1 ml from each dilution was plated in duplicate on BA plates.…”

Section: In Vitro Survival Of Gbs In Ensmentioning

confidence: 99%

“…There were 39 septic deaths. During the 7-day ingestion interval 15 died and 24 died within 10 days after exposure. The risk of sepsis was highest for young mice.…”

mentioning

confidence: 99%

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Bacterial Contamination of Enteral Nutrient Solutions: Intestinal Colonization and Sepsis in Mice after Ingestion

Enk

Furtado

1986

J Parenter Enteral Nutr

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Contaminated enteral nutrient solution (ENS) was used to assess the risks of intestinal colonization and invasive sepsis using normal mice. Dilute Osmolite containing 10(6) Group B Streptococci/ml given for 2 to 10 consecutive days resulted in the detection of organisms by rectal washing sampling and in the recovery of 10(3) to 10(4) organisms from the cecum, large intestine, and rectum. Only 10(1) organisms survived in the small intestine. Colonization, or the persistence of organisms for 10 days after exposure, was produced after 2 days of ingestion in 44% of animals and in 100% after exposure for 5 days. There were 39 septic deaths. During the 7-day ingestion interval 15 died and 24 died within 10 days after exposure. The risk of sepsis was highest for young mice. Ways to minimize bacterial contamination of ENS intended for administration to patients are discussed.

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Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Osmolitementioning

confidence: 99%

Section: In Vitro Survival Of Gbs In Ensmentioning

confidence: 99%

“…There were 39 septic deaths. During the 7-day ingestion interval 15 died and 24 died within 10 days after exposure. The risk of sepsis was highest for young mice.…”

mentioning

confidence: 99%

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