Bacterial contamination of drinking water sources in rural villages of Mohale Basin, Lesotho: exposures through neighbourhood sanitation and hygiene practices

Gwimbi, Patrick; George, Maeti; Ramphalile, Motena

doi:10.1186/s12199-019-0790-z

Cited by 94 publications

(85 citation statements)

References 18 publications

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“…In Africa, roughly 40% of the population does not have access to improved water supply and sanitation [6]. Study conducted in rural villages of Mohale Basin in Lesotho show that drinking water was polluted by Escherichia coli (78% of unprotected water and 60% of protected water sources) and 59% of water sample contain open defecation with poor control of hygiene practice [7].…”

Section: Introductionmentioning

confidence: 99%

Quality and safety of municipal drinking water in Addis Ababa City, Ethiopia

Wolde

Jemal²,

Woldearegay

et al. 2020

Environ Health Prev Med

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Background:In low resourced countries, water-associated diseases have still impact on public health. Poor quality of water can cause waterborne diseases through bacteria, viruses, protozoa, and parasites that has been responsible for millions of morbidity and mortality. Therefore, this study aimed to assess quality and safety of public municipal drinking water in Addis Ababa City. Methods: Descriptive epidemiological study design that used quantitative approach was carried out at Addis Ababa City Administration from June 2016 to October 2016. Pre-tested and standardized aseptic sample collection technique was utilized to collect a total of 2976 samples (2951 water samples for bacteriological analysis by Presence-Absence (P-A) culturing method and 25 samples for parasites identification through direct microscopy examination). Descriptive data were summarized and cleaned by the SPSS version 20 software and presented in table and graph. Results: The study revealed that 10%, 7% and 3% were positive for bacteriological, total coliforms, and fecal coliforms respectively through Presence-Absence Broth test. The bacterial distribution trends from 1st to 13th weeks of wet season were slight increment of total coliforms and slight decrement for fecal coliforms. All tested for parasitological samples from selected reservoirs were free from parasitological species. Conclusion: This study reflects that there were positive for bacterial, total coliforms, and fecal coliforms during the study period. It needs continuous screening and treating water sources to utmost important for prevention and control waterborne disease.

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Section: Introductionmentioning

confidence: 99%

Quality and safety of municipal drinking water in Addis Ababa City, Ethiopia

Wolde

Jemal²,

Woldearegay

et al. 2020

Environ Health Prev Med

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show abstract

“…In fact, work by Gwimbi et al shows that contaminated water sources in locations such as rural Lesotho have coliform concentrations as high as 10 5 CFU/mL. 11 This is within the range of E. coli concentrations where our biosensors have been observed to function, suggesting that the LOD of our biosensors is low enough to be used in real-world applications.…”

Section: Discussionmentioning

confidence: 64%

Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications

Bruce¹,

Clapper²

2020

Preprint

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Quantum dots have proven to be strong candidates for biosensing applications in recent years, due to their strong light emission properties and their ability to be modified with a variety of functional groups for the detection of different analytes. Here, we investigate the use of conjugated carboxylated graphene quantum dots (CGQDs) for the detection of E. coli, using a biosensing procedure that focuses on measuring changes in fluorescence quenching. Our CGQDs were conjugated with cecropin P1, a naturally-produced antibacterial peptide that facilitates the attachment of CGQDs to E. coli cells. We also confirm the structural modification of these conjugated CGQDs in addition to analyzing their optical characteristics. Our findings have the potential to be used in situations where rapid, reliable detection of bacteria in liquids, such as drinking water, is required, especially given our biosensor’s relatively low observed limit of detection (LOD).

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“…33 , 34 However, data from various published studies show that contaminated water sources can have much higher concentrations of E. coli and total coliforms, very often ranging from 10 2 to 10 5 CFU/mL in locations such as Lesotho, Gabon, Kenya, Slovakia, Ivory Coast, and Nepal. 35 − 40 …”

Section: Discussionmentioning

confidence: 99%

Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications

Bruce¹,

Clapper²

2020

ACS Omega

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Biosensors that can accurately and rapidly detect bacterial concentrations in solution are important for potential applications such as assessing drinking water safety. Meanwhile, quantum dots have proven to be strong candidates for biosensing applications in recent years because of their strong light emission properties and their ability to be modified with a variety of functional groups for the detection of different analytes. Here, we investigate the use of conjugated carboxylated graphene quantum dots (CGQDs) for the detection of Escherichia coli using a biosensing assay that focuses on measuring changes in fluorescence intensity. We have further developed this assay into a novel, compact, field-deployable biosensor focused on rapidly measuring changes in absorbance to determine E. coli concentrations. Our CGQDs were conjugated with cecropin P1, a naturally produced antibacterial peptide that facilitates the attachment of CGQDs to E. coli cells; to our knowledge, this is the first instance of cecropin P1 being used as a biorecognition element for quantum dot biosensors. As such, we confirm the structural modification of these conjugated CGQDs in addition to analyzing their optical characteristics. Our findings have the potential to be used in situations where rapid, reliable detection of bacteria in liquids, such as drinking water, is required, especially given the low range of E. coli concentrations (10 3 to 10 6 CFU/mL) within which our two biosensing assays have collectively been shown to function.

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Bacterial contamination of drinking water sources in rural villages of Mohale Basin, Lesotho: exposures through neighbourhood sanitation and hygiene practices

Cited by 94 publications

References 18 publications

Quality and safety of municipal drinking water in Addis Ababa City, Ethiopia

Quality and safety of municipal drinking water in Addis Ababa City, Ethiopia

Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications

Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications

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