Bacterial composition and red fluorescence of plaque in relation to primary and secondary caries next to composite: an in situ study

Thomas, Renske Z.; Mei, H. C. Van Der; Veen, Monique H. van der; Soet, J.J. de; Huysmans, M.C.D.N.J.M.

doi:10.1111/j.1399-302x.2007.00381.x

Cited by 67 publications

(78 citation statements)

References 27 publications

(38 reference statements)

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“…Maximum in fluorescence emission coincided with maximum in the bacterial physical growth curve on agar plates [17]. That red fluorescence started to increase in day four suggests a behavior in accordance to statements that mature plaque is the source of red fluorescence emission in the oral cavity [6][7][8][9]. In addition, according to the literature, it is important not to disturb growing cultures during the initial "lag phase" when they are becoming acclimatized to the environment [17].…”

Section: Discussionmentioning

confidence: 74%

“…Van der Veen et al put forward that distinct red fluorescence from Peptostreptococcus micros only was observed when grown in close proximity of P. gingivalis [6] and proposed that it is the intrinsic bacterial mixes of the mature biofilm, rather than single bacterium species that are responsible for the red fluorescence. Other studies have supported this suggestion regarding the importance of the mature biofilm for the emission of red fluorescence [7][8][9]. One of the authors proposed that it is due to signaling and synergy between different bacterial species in the biofilm rather than the characteristics of the single species [8].…”

Section: Introductionmentioning

confidence: 60%

“…Red fluorescence emission has been demonstrated from Actinomyces odontolyticus, Actinomyces naeslundi, Actinomyces israelii and Prevotella intermedia present in cell cultures associated with dental caries, periodontal disease and dental plaque [3,[5][6][7]. Red fluorescence has also been obtained from the black-pigmented obligate anaerobes Porphyromonas gingivalis, P. intermedia, Prevotella melaninogenica, as well as from A. israelii [8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

On the red fluorescence emission of Aggregatibacter actinomycetemcomitans

Bjurshammar¹,

Johannsen²,

Buhlin³

et al. 2012

OJST

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A number of studies have indicated that bacteria able to emit red fluorescence can be detected by light induced fluorescence technique and killed by photodynamic therapy. The objective of this study was to investigate the red fluorescence properties of the single gram negative capnophilic bacterium Aggregatibacter actinomycetemcomitans, ATCC 33384, and to investigate if these properties were related to the growth, morphology and size of the bacterial colonies. Time trend assessment was made with red fluorescence by QLF (Quantitative Light-induced Fluorescence), as well as with white light digital imaging. It was demonstrated that A. actinomycetemcomitans, a single capnophilic bacterium, is able to produce red fluorescence on its own, i.e. in the absence of other bacteria strains, and that blood agar is necessary to obtain red fluorescence from this bacterium on culture plates. This bacterium formed smooth circular, bell/dome like colonies increasing in size with time exhibiting various red fluorescence behaviors. A large variation in the fluorescence behavior points out an inhomogeneous distribution of red fluorescence within and between the colonies, i.e. the size of the investigated colonies did not correlate with the red fluorescence area, suggesting a dependence on the colony morphology such as the colony growth in height. To our knowledge this is the first study that have shown that A. actinomycetemcomitans on its own is able to produce fluorescence in the red spectral region.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

On the red fluorescence emission of Aggregatibacter actinomycetemcomitans

Bjurshammar¹,

Johannsen²,

Buhlin³

et al. 2012

OJST

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show abstract

“…43,44 Current research on RF focuses mainly on bacteria that could be a source for this RF and bacteria interactions and growth conditions. 10,43,45,46 Recently a more clinical approach is found in research of RF in relation to gingivitis, where not the red or GF of the teeth is the object of study but the RF seen from the subgingival plaque growing under the gums.…”

Section: Clinical Resultsmentioning

confidence: 99%

Quantified light-induced fluorescence, review of a diagnostic tool in prevention of oral disease

Jong

Higham

Smith

et al. 2009

Journal of Applied Physics

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Diagnostic methods for the use in preventive dentistry are being developed continuously. Few of these find their way into general practice. Although the general trend in medicine is to focus on disease prevention and early diagnostics, in dentistry this is still not the case. Nevertheless, in dental research some of these methods seem to be promising for near future use by the general dental professional. In this paper an overview is given of a method called quantitative light-induced fluorescence or (QLF) in which visible and harmless light excites the teeth in the patient's mouth to produce fluorescent images, which can be stored on disk and computer analyzed. White spots (early dental caries) are detected and quantified as well as bacterial metabolites on and in the teeth. An overview of research to validate the technique and modeling to further the understanding of the technique by Monte Carlo simulation is given and it is shown that the fluorescence phenomena can be described by the simulation model in a qualitative way. A model describing the visibility of red fluorescence from within the dental tissue is added, as this was still lacking in current literature. An overview is given of the clinical images made with the system and of the extensive research which has been done. The QLF™ technology has been shown to be of importance when used in clinical trials with respect to the testing of toothpastes and preventive treatments. It is expected that the QLF™ technology will soon find its way into the general dental practice.

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“…A general belief is that the cariogenic biofilm for primary and secondary caries are similar, and consist mainly of Streptococcus mutans, Lactobacilli and Actinomyces naeslundii [65][66][67], although a contrary opinion based on observations made in an in situ experiment has been proposed [68].…”

Section: Etiological Factorsmentioning

confidence: 99%

Secondary caries and microleakage

Jokstad

2016

Dental Materials

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Objectives: To critically appraise experimental ex vivo research that has focussed on secondary caries, and to offer possible explanations for the seemingly poor correlation to clinical observations. Methods: The literature relating to the etiopathogenesis or prevention of secondary caries gained from experimental ex vivo research was reviewed, with particular emphasis on microleakage and artificial caries-like lesions.Results: It is doubtful whether a caries wall lesion can exist independently of an outer enamel caries lesion. Microleakage experiments apparently continue to emerge regardless of multiple reviews questioning the reliability and validity of the method. Several of the approaches used to generate artificial caries-like lesions are very aggressive. Remarkably little discussion has evolved about how these aggressive approaches create microenvironments that do not occur in reality. Corrosion-and biodegradation products may influence the biofilm qualitatively and quantitatively and it is difficult to replicate these variables in any ex vivo environment. Clinical data sampling method, patient demography as well as study methodology influences the incidence and prevalence estimates of secondary caries. Clinical results based on clinical work in settings where cost per unit time is of nominal concern do not provide any indications on how the restorative material will perform when placed by the average dentists in the mouths of their spectrum of patients during a busy workday.Recommendations: The term "wall lesion" including its variants is ill defined, has been, and is still being used indiscriminately. Stakeholders should avoid using this ambiguous label due to its connotation to an entity that does not exist per se.

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Bacterial composition and red fluorescence of plaque in relation to primary and secondary caries next to composite: an in situ study

Abstract: The higher proportion of caries-associated bacteria on restored tissue indicates that the ecology on the surface of primary lesions differs from that on lesions next to composite, and that secondary caries next to composite may differ from the primary caries process.

Cited by 67 publications

References 27 publications

On the red fluorescence emission of <i>Aggregatibacter actinomycetemcomitans</i>

On the red fluorescence emission of <i>Aggregatibacter actinomycetemcomitans</i>

Quantified light-induced fluorescence, review of a diagnostic tool in prevention of oral disease

Secondary caries and microleakage

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Bacterial composition and red fluorescence of plaque in relation to primary and secondary caries next to composite: an in situ study

Abstract: The higher proportion of caries-associated bacteria on restored tissue indicates that the ecology on the surface of primary lesions differs from that on lesions next to composite, and that secondary caries next to composite may differ from the primary caries process.

Cited by 67 publications

References 27 publications

On the red fluorescence emission of &lt;i&gt;Aggregatibacter actinomycetemcomitans&lt;/i&gt;

On the red fluorescence emission of &lt;i&gt;Aggregatibacter actinomycetemcomitans&lt;/i&gt;

Quantified light-induced fluorescence, review of a diagnostic tool in prevention of oral disease

Secondary caries and microleakage

Contact Info

Product

Resources

About

On the red fluorescence emission of <i>Aggregatibacter actinomycetemcomitans</i>

On the red fluorescence emission of <i>Aggregatibacter actinomycetemcomitans</i>