Bacterial community diversity in cultures derived from healthy and inflamed ileal pouches after restorative proctocolectomy

Johnson, Matt; Rogers, Geraint B.; Bruce, Kenneth D.; Lilley, Andrew K.; Herbay, A. von; Forbes, Alastair; Ciclitira, Paul J.; Nicholls, R. J.

doi:10.1002/ibd.21022

Cited by 14 publications

(11 citation statements)

References 57 publications

(78 reference statements)

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“…Mucosal biopsies were first cultured on agar, the bacterial DNA was then extracted and amplified using PCR and profiles were generated using TRFLP [Johnson et al 2009]. No differences between pouchitis and nonpouchitis groups or between FAP and UC groups were found but this study also had significant limitations.…”

Section: Studies Using Molecular Methodsmentioning

confidence: 98%

The bacterial pathogenesis and treatment of pouchitis

McLaughlin

Clark

Tekkis

et al. 2010

Therap Adv Gastroenterol

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Restorative proctocolectomy (RPC) with ileal pouch-anal anastomosis is the operation of choice for patients with ulcerative colitis. Pouchitis is the most common cause of pouch dysfunction. Although the pathogenesis of this disease is not well understood, bacteria have been implicated in the disease process. Numerous bacterial studies have been reported over the last 25 years with few unifying findings. In addition, many different treatments for pouchitis have been reported with varying results. Antibiotic treatment remains the most studied and is the mainstay of treatment. In this article we review the aetiology of pouchitis and the evidenced-based treatment options.

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Section: Studies Using Molecular Methodsmentioning

confidence: 98%

The bacterial pathogenesis and treatment of pouchitis

McLaughlin

Clark

Tekkis

et al. 2010

Therap Adv Gastroenterol

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“…Many investigators report a reduction in bacterial diversity in samples from IBD patients, often with increased Enterobacteriaceae , including E. coli , and a reduction in Firmicutes including Clostridia(11-15). Others have reported no differences in diversity(12;16) or a non-significant increase(17). Both increased(15;18-20) and decreased(21;22) levels of Bacteroidetes have been reported, likewise some studies report differences in microbiota in active and inactive disease(19;21;23), whereas others do not(18).…”

Section: Introductionmentioning

confidence: 96%

“…The second (from our unit) studied 32 RPC patients using terminal-restriction fragment length polymorphism (TRFLP). No differences between pouchitis and non-pouchitis groups were found(17), Both of these studies, however, had limitations. First, the technique used by Johnson et al (17) was limited to only being able to identify dominant species groups and was not able to identify individual bacterial species.…”

Section: Introductionmentioning

confidence: 98%

The Bacteriology of Pouchitis

McLaughlin¹,

Walker²,

Churcher³

et al. 2010

Annals of Surgery

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103

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Objective-To identify, compare and contrast the microbiota in patients with and without pouchitis after RPC for UC and FAP.Summary Background Data-Pouchitis is the most common complication following restorative proctocolectomy (RPC). An abnormal host-microbial interaction has been implicated. We investigated the pouch microbiota in patients with and without pouchitis undergoing restorative proctocolectomy for UC and familial adenomatous polyposis (FAP).Methods-Mucosal pouch biopsies, taken from 16 UC (pouchitis 8) and 8 FAP (pouchitis 3) patients were analysed to the species (or phylotype) level by cloning and sequencing of 3,184 fulllength bacterial 16S rRNA genes. All authors declare no conflicts of interest exist. There are no financial disclosures for any author. Sequence Submission: (GenBank Accession numbers GQ156578-GQ159761)This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Results-There was a significant increase in Proteobacteria (p= 0.019) and a significant decrease in Bacteroidetes (p= 0.001) and Faecalibacterium prausnitzii (p=0.029) in the total UC compared to the total FAP cohort, but only limited differences were found between the UC non-pouchitis and pouchitis groups and the FAP pouchitis and non-pouchitis groups. Bacterial diversity in the FAP non-pouchitis group was significantly greater than in UC non-pouchitis (p= 0.019) and significantly greater in UC non-pouchitis compared to UC pouchitis (p= 0.009). No individual species or phylotype specifically associated with either UC or FAP pouchitis was found. Europe PMC Funders GroupConclusions-UC pouch patients have a different, less diverse, gut microbiota than FAP patients. A further reduction in bacterial diversity but no significant dysbiosis occurs in those with pouchitis. The study suggests that a dysbiosis occurs in the ileal pouch of UC RPC patients which predisposes to, but may not directly cause, pouchitis.

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“…Studies have shown variation in the microbiota in pouchitis and healthy controls; however, based on different culture methods and molecular biology techniques, no consensus was available[3]. Johnson et al[5] and Lim et al[6] showed no differences between pouchitis and no pouchitis (NP) groups. Some studies have suggested a reduction in bacterial diversity in pouchitis but not dysbiosis[7].…”

Section: Introductionmentioning

confidence: 99%

Fecal microbiota in pouchitis and ulcerative colitis

Wang

Wei

et al. 2016

WJG

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AIMTo investigate the changes in microbiota in feces of patients with ulcerative colitis (UC) and pouchitis using genomic technology.METHODSFecal samples were obtained from UC patients with or without an ileal pouch-anal anastomosis (IPAA) procedure, as well as healthy controls. The touchdown polymerase chain reaction technique was used to amplify the whole V3 region of the 16S rRNA gene, which was transcribed from DNA extracted from fecal samples. Denaturing gradient gel electrophoresis was used to separate the amplicons. The band profiles and similarity indices were analyzed digitally. The predominant microbiota in different groups was confirmed by sequencing the 16S rRNA gene.RESULTSMicrobial biodiversity in the healthy controls was significantly higher compared with the UC groups (P < 0.001) and IPAA groups (P < 0.001). Compared with healthy controls, the UC patients in remission and those in the mildly active stage, the predominant species in patients with moderately and severely active UC changed obviously. In addition, the proportion of the dominant microbiota, which was negatively correlated with the disease activity of UC (r = -6.591, P < 0.01), was decreased in pouchitis patients. The numbers of two types of bacteria, Faecalibacterium prausnitzii and Eubacterium rectale, were reduced in UC. Patients with pouchitis had an altered microbiota composition compared with UC patients. The microbiota from pouchitis patients was less diverse than that from severely active UC patients. Sequencing results showed that similar microbiota, such as Clostridium perfringens, were shared in both UC and pouchitis.CONCLUSIONLess diverse fecal microbiota was present in patients with UC and pouchitis. Increased C. perfringens in feces suggest its role in the exacerbation of UC and pouchitis.

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Bacterial community diversity in cultures derived from healthy and inflamed ileal pouches after restorative proctocolectomy

Abstract: No significant differences were found between the bacterial cultures derived from any of the clinical groups or between pouchitis and nonpouchitis patients.

Cited by 14 publications

References 57 publications

The bacterial pathogenesis and treatment of pouchitis

The bacterial pathogenesis and treatment of pouchitis

The Bacteriology of Pouchitis

Fecal microbiota in pouchitis and ulcerative colitis

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