Bacterial cellulose production byAcetobacter xylinum in a 50-L internal-loop airlift reactor

Chao, Yaping; Ishida, Takehiko; Sugano, Yasushi; Shoda, Makoto

doi:10.1002/(sici)1097-0290(20000505)68:3<345::aid-bit13>3.0.co;2-m

Cited by 126 publications

(43 citation statements)

References 28 publications

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“…The productivity of BC in the 10 L spherical type bubble column bioreactor in this research was similar to that of our previous report [7]. Therefore, the mass production of BC was considered successful.…”

Section: Construction Of 50 L Spherical Type Bubble Column Bioreactorsupporting

confidence: 89%

“…The supernatant of the saccharified food wastes was used as the BC production medium, with HS (Hestrin & Schramm) complex [12] and CSL-Fru (Corn steep liquor-fructose) media [7] used as the control; the HS medium was used during the early research on the production of BC, with CSL-Fru medium, recently developed at the Shoda laboratory at the Tokyo Institute of Technology, Japan, used as the optimized complex medium.…”

Section: Bc Producer and Bc Production Mediummentioning

confidence: 99%

“…Usually, BC is produced as the pellicle type on the surface of the culture under static culture conditions. However, the traditional static culture method cannot be applied to mass production, as it requires a long culture period and is labor intensive, resulting in low productivity, and therefore is not effective for industrial production [7]. Also, the production of BC by the Acetobacter strain requires low shear stress and a high oxygen transfer rate.…”

Section: Introductionmentioning

confidence: 99%

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Pilot-scale production of bacterial cellulose by a spherical type bubble column bioreactor using saccharified food wastes

Song

Seo

et al. 2009

Korean J. Chem. Eng.

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Bacterial cellulose (BC) was produced by Acetobacter xylinum KJ1 in a modified airlift-type bubble column bioreactor, which had a low shear stress and high oxygen transfer rate (k L a). Saccharified food wastes (SFW) were used as the BC production medium due to its low cost. An aeration rate of 1.2 vvm (6 L/min) was tentatively determined as the optimal aeration condition in a 10 L spherical type bubble column bioreactor, by analysis of the oxygen transfer coefficient. When 0.4% agar was added, the BC production reached 5.8 g/L, compared with 5.0 g/L in the culture without the addition of agar. The BC productivity was improved by 10% in the addition of 0.4% agar into the SFW medium. Then, by conversion of a linear velocity of 0.93 cm/sec, from the relationship between the linear velocity and oxygen transfer rate, 1.0 vvm (30 L/min) was determined as an optimal aeration condition in a 50 L spherical type bubble column reactor. Using SFW medium, with the addition of 0.4% agar, and air supplied of 1.0 vvm, 5.6 g/L BC was produced in the 50 L spherical type bubble column bioreactor after 3 days of cultivation, which was similar to that produced in the 10 L bioreactor. In conclusion, the addition of agar, a viscous polysaccharide, into SFW medium is effective for the production of BC, and this scale-up method is very useful for the mass production in a 50 L spherical type bubble column bioreactor by decreasing the shear stress and increasing the k L a.

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Section: Construction Of 50 L Spherical Type Bubble Column Bioreactorsupporting

confidence: 89%

Section: Bc Producer and Bc Production Mediummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pilot-scale production of bacterial cellulose by a spherical type bubble column bioreactor using saccharified food wastes

Song

Seo

et al. 2009

Korean J. Chem. Eng.

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“…The widely used medium of fructose and corn steep liquor was used in shake cultures achieving production of 5-8 g/L [11]. Furthermore, a production of 7-9.2 g/L was achieved in conventional stirred tank bioreactors, while lower yields of 5.6-6.4 g/L were obtained in airlift bioreactors [9,[11][12][13]. D-xylose medium has also been used to produce BC, which achieved lower production rates than fructose [14].…”

Section: Introductionmentioning

confidence: 99%

Potential of Biocellulose Nanofibers Production from Agricultural Renewable Resources: Preliminary Study

Dahman

Jayasuriya

Kalis

2010

Appl Biochem Biotechnol

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In the present preliminary study, we report results for the biocellulose nanofibres production by Gluconacetobacter xylinus. Production was examined by utilizing different feedstocks of single sugars and sugar mixtures with compositions similar to the acid hydrolyzates of different agriculture residues. Profiles for cell proliferation, sugar consumption, and the subsequent pH changes were thoroughly analyzed. Highest biocellulose production of 5.65 g/L was achieved in fructose medium with total sugar consumption of 95.57%. Moreover, the highest production using sugar mixtures was 5.2 g/L, which was achieved in feedstock with composition identical to the acid hydrolyzate of wheat straws. This represented the highest biocellulose yield of 17.72 g/g sugars compared with 14.77 g/g fructose. The lowest production of 1.1 and 1.75 g/L were obtained in xylose and glucose media, respectively, while sucrose and arabinose media achieved relatively higher production of 4.7 and 4.1 g/L, respectively. Deviation in pH of the fermentation broths from the optimum value of 4-5 generally had marked effect on biocellulose production with single sugars in feedstock. However, the final pH values recorded in the different sugar mixtures were approximately 3.3-3.4, which had lower effect on production hindrance. Analyzing profiles for sugars' concentrations and cell growth showed that large amount of the metabolized sugars were mainly utilized for bacterial cell growth and maintenance, rather than biocellulose production. This was clearly observed with single sugars of low production, while sugar consumption was rather utilized for biocellulose production with sugar mixtures. Results reported in this study demonstrate that agriculture residues might be used as potential feedstocks for the biocellulose nanofibres production. Not only this represents a renewable source of feedstock, but also might lead to major improvements in production if proper supplements and control were utilized in the fermentation process.

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“…And Chao etc. [13] also reported that it was 8.0 g/L in an airlift reactor using air and oxygen for 67 hours culture. These productivities are almost at a similar level to ours.…”

Section: Productivity Of Bc Depending Upon the Culture Modesmentioning

confidence: 98%

Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor

Choi

Song

Kim

et al. 2009

Korean J. Chem. Eng.

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The saccharogenic liquid obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes (SFW) was carried out by the cultivation supernatant of Trichoderma inhamatum KSJ1. 5.6 g/L of BC was produced in a new modified 50 L bubble column bioreactor by Acetobacter xylinum KJ1. The productivity was similar to that of a modified 10 L bubble column bioreactor (5.8 g/L). When pure oxygen was supplied into the scaled-up culture conditions, 6.8 g/L (12% enhancement) of BC was produced, indicating a very useful method for BC mass production. The oxygen uptake rate (OUR) and q O2 (specific oxygen uptake rate) were 0.214 mg-DO/L·min and 0.257 mg-DO/g-cell·min, respectively. The physical properties, such as morphology, molecular weight, crystallinity, and tensile strength of BCs produced in static culture (A), 10 L (B) and 50 L (C) modified bubble column cultures were investigated. All BCs showed fibrils with highly networking structure. The number average molecular weight of BCs in A, B and C was 2,314,000, 1,878,000, and 1,765,000, respectively. All of the BCs had a form of cellulose I representing pure cellulose. The relative degree of crystallinity showed the range of 79.6-86.0%. Tensile strengths of BC sheet in A, B and C were 1.75, 1.21, and 1.19 kg/mm 2 , respectively. In conclusion, BC production by the modified bubble column culture mode of 50 L brought more favorable results in terms of the physical properties and its ease of scale-up.

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Bacterial cellulose production byAcetobacter xylinum in a 50-L internal-loop airlift reactor

Cited by 126 publications

References 28 publications

Pilot-scale production of bacterial cellulose by a spherical type bubble column bioreactor using saccharified food wastes

Pilot-scale production of bacterial cellulose by a spherical type bubble column bioreactor using saccharified food wastes

Potential of Biocellulose Nanofibers Production from Agricultural Renewable Resources: Preliminary Study

Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor

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