Bacterial Blight and Dieback of <i>Eucalyptus</i> Species, Hybrids, and Clones in South Africa

Coutinho, Teresa A.; Preisig, Oliver; Mergaert, Joris; Cnockaert, Margo; Riedel, Kristina; Swings, Jean; Wingfield, M.J.

doi:10.1094/pdis.2002.86.1.20

Cited by 80 publications

(91 citation statements)

References 20 publications

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“…16S rRNA gene sequencing and multilocus sequence analyses (MLSA) revealed that the strains were phylogenetically closely related to Pantoea agglomerans, Pantoea stewartii and Pantoea ananatis. P. ananatis is the causal agent of bacterial blight and dieback of eucalyptus in South Africa (Coutinho et al, 2002). In 2001, Pantoea-like strains were isolated from eucalyptus trees exhibiting a similar disease in Uganda, Argentina and Uruguay and were thought to belong to P. ananatis.…”

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“…Strains of Pantoea are diverse in their origin and geographical spread, and have been isolated from plants as well as from clinical samples. P. ananatis is the causal agent of bacterial blight and dieback of eucalyptus in South Africa (Coutinho et al, 2002). In 2001, Pantoea-like strains were isolated from eucalyptus trees exhibiting a similar disease in Uganda, Argentina and Uruguay and were thought to belong to P. ananatis.…”

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Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.

Brady

Venter

Cleenwerck

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in Uganda, Uruguay and Argentina and from maize displaying brown stalk rot symptoms in South Africa were tentatively placed in the genus Pantoea on the basis of phenotypic and biochemical tests. These isolates, together with two strains (LMG 2558 and LMG 2560) previously assigned to Pantoea agglomerans based on protein electrophoregrams but later excluded from this species, were further investigated using molecular techniques. 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) revealed that the strains were phylogenetically closely related to Pantoea agglomerans, Pantoea stewartii and Pantoea ananatis. P. ananatis is the causal agent of bacterial blight and dieback of eucalyptus in South Africa (Coutinho et al., 2002). In 2001, Pantoea-like strains were isolated from eucalyptus trees exhibiting a similar disease in Uganda, Argentina and Uruguay and were thought to belong to P. ananatis. P. ananatis was also found to cause brown stalk rot of maize in South Africa (Goszczynska et al., 2007). Isolated simultaneously with P. ananatis from maize were additional Pantoea-like strains which also caused brown stalk rot. Multilocus sequence analysis (MLSA) has been shown to separate the isolates from eucalyptus and maize into three clusters, indicating that these strains probably constitute three novel species of Pantoea (Brady et al., 2008). Also included in the MLSA study were two strains, LMG 2558 (5NCPPB 1682) and LMG 2560 (5NCPPB 1941), mentioned by Beji et al. (1988) as belonging to protein profiling group VII, which were assigned to P. agglomerans based on their electrophoregrams, despite never being included in DNA-DNA hybridization experiments with the type strain of P. agglomerans. It was suggested by Gavini et al. (1989) that strains of protein Abbreviations: AFLP, amplified fragment length polymorphism; MLSA, multilocus sequence analysis.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of P. eucalypti R-25678 T (5LMG 24197 T ), P. anthophila LMG 2558 T , P. deleyi R-31523 T (5LMG 24200 T ) and P. vagans R-21566 T (5LMG 24199 T ) are EF688009-EF688012, respectively, and those of strains LMG 5345, LMG 5343, LMG 2781 and R-35496 are respectively EU216734-EU216737.Detailed DNA-DNA hybridization results and a UPGMA dendrogram based on FAFLP analysis are available as supplementary material with the online version of this paper.

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confidence: 99%

mentioning

confidence: 99%

Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.

Brady

Venter

Cleenwerck

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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122

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“…En Brasil, las especies bacterianas asociadas a manchas foliares incluyen a Erwinia psidii Rodrigues Neto, Robbs et Yamashiro, Pseudomonas cichorii (Swingle) Stapp, Rhizobiaceae, y Xanthomonas axonopodis Starr et Garces, , Gonçalves et al 2008, siendo X. axonopodis la especie predominante en Brasil (Gonçalves et al 2008). En Sudáfrica se asocia a Pantoea agglomerans (Beijerinck) Gavini et al y Pantoea ananatis (Serra-no) Mergaert et al con esta enfermedad (Coutinho et al 2002) al igual que en Uruguay (FAO 2006).…”

Section: Manchas Foliares Bacterianasunclassified

“…Con el tiempo las lesiones se fusionan, conduciendo a la caída prematura de las hojas provocando defoliación (Neves 2007, Gonçalves et al 2008, Alfenas et al 2009) (figura 1). En Sudáfrica y Uruguay se han descrito síntomas que incluyen hojas con aspecto reseco, concentrados principalmente sobre nervadura principal y pecíolo (Coutinho et al 2002). En Argentina y Paraguay, Ferreira et al (2001) describieron manchas bacterianas como lesiones angulares dispersas en todo el limbo, mayormente concentradas en los bordes de las hojas y próximo a la nervadura principal.…”

Section: Manchas Foliares Bacterianasunclassified

“…En Sudáfrica se identificó a Pantoea ananatis causando síntomas similares a los descritos para X. campestris pv. eucalypti por Truman (1974) (Coutinho et al 2002) y más recientemente en el año 2014 a Xanthomonas vasicola Vauterin (Coutinho et al 2014). En Argentina y Brasil, se ha identificado a Erwinia psidii como agente causal del tizón apical bacteriano (Arriel et al 2014, Coutinho et al 2011.…”

Section: Tizón Apical Bacterianounclassified

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Enfermedades bacterianas de eucaliptos: Estado actual del conocimiento

Palladino

Pérez

2016

Bosque (Valdivia)

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SUMMARYIn recent years there has been a substantial increase in health problems on Eucalyptus spp. plantations elsewhere. Several diseases are currently affecting this crop, predominantly those caused by fungi. However, bacterial diseases have globally increased in importance. Eucalyptus plant pathogenic bacteria can cause: i) leaf spots, ii) shoot blight and iii) vascular wilt. Bacterial leaf spots have been associated with genera Xanthomonas, Pseudomonas, Erwinia, Pantoea and Rhizobiaceae, being Xanthomonas axonopodis the predominant species. Bacterial die-back of bacterial shoot blight can be caused by several species, namely Erwinia psidii, Pantoea ananatis, Enterobacter cowanii, Xanthomonas campestris and X. vasicola, whereas vascular wilt has been associated with Ralstonia solanacearum and Erwinia psidii. These diseases represent a potential risk for forestry production, as reported in different climatic zones worldwide causing serious economic loss, in both nursery and field, affecting a wide host range in the genus Eucalyptus, and some species of Psidium. In addition, the effective dissemination of these bacterial diseases makes controlling them difficult, thus the deployment of genetic resistance germplasm is strongly recommended to minimize the impact of these diseases.Key words: plant pathogenic bacteria, leaf spots, die-back, vascular wilt. RESUMENEn los últimos años se ha observado un aumento sustantivo de las problemáticas sanitarias en las plantaciones de Eucalyptus spp. (eucalipto) Diversas enfermedades afectan actualmente a este cultivo, predominando aquellas causadas por hongos. Sin embargo, las enfermedades de origen bacteriano han aumentado su importancia a nivel mundial. Las bacterias fitopatógenas en eucalipto pueden causar: i) manchas foliares, ii) tizón apical y iii) marchitamiento vascular. En el mundo, las manchas foliares bacterianas han sido asociadas a los géneros Xanthomonas, Pseudomonas, Erwinia, Pantoea y Rhizobiaceae, siendo Xanthomonas axonopodis la especie predominante. El tizón bacteriano puede ser causado por distintas especies bacterianas, Erwinia psidii, Pantoea ananatis, Enterobacter cowanii, Xanthomonas campestris y X. vasicola. Mientras que el marchitamiento vascular ha sido asociado a Ralstonia solanacearum y Erwinia psidii. Estas enfermedades representan un potencial riesgo para la producción forestal, ya que han sido reportadas en diversas zonas climáticas del mundo ocasionando graves pérdidas económicas, tanto en vivero como en campo, pudiendo afectar un amplio rango de hospederos dentro del género Eucalyptus, como también algunas especies del género Psidium. Sumado a esto, las bacterias se diseminan con facilidad dificultando su control, por lo que la principal medida de manejo es la utilización de germoplasma con mejor comportamiento sanitario.Palabras clave: bacterias fitopatógenas, manchas foliares, tizón apical, marchitamiento vascular. INTRODUCCIÓNLa superficie plantada con eucalipto (Eucalyptus spp.) ha ido en aumento a nivel mundial, superando actualmente los...

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Investigation of a sugar N‐formyltransferase from the plant pathogen Pantoea ananatis

2019

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Pantoea ananatis is a Gram-negative bacterium first recognized in 1928 as the causative agent of pineapple rot in the Philippines. Since then various strains of the organism have been implicated in the devastation of agriculturally important crops. Some strains, however, have been shown to function as non-pathogenic plant growth promoting organisms. To date, the factors that determine pathogenicity or lack thereof between the various strains are not well understood. All P. ananatis strains contain lipopolysaccharides, which differ with respect to the identities of their associated sugars. Given our research interest on the presence of the unusual sugar, 4-formamido-4,6-dideoxy-Dglucose, found on the lipopolysaccharides of Campylobacter jejuni and Francisella tularensis, we were curious as to whether other bacteria have the appropriate biosynthetic machinery to produce these unique carbohydrates. Four enzymes are typically required for their biosynthesis: a thymidylyltransferase, a 4,6-dehydratase, an aminotransferase, and an N-formyltransferase. Here, we report that the gene SAMN03097714_1080 from the P. ananatis strain NFR11 does, indeed, encode for an N-formyltransferase, hereafter referred to as PA1080c. Our kinetic analysis demonstrates that PA1080c displays classical Michaelis-Menten kinetics with dTDP-4-amino-4,6-dideoxy-D-glucose as the substrate and N 10 -formyltetrahydrofolate as the carbon source. In addition, the X-ray structure of PA1080c, determined to 1.7 Å resolution, shows that the enzyme adopts the molecular architecture observed for other sugar N-formyltransferases. Analysis of the P. ananatis NFR11 genome suggests that the three other enzymes necessary for N-formylated sugar biosynthesis are also present. Intriguingly, those strains of P. ananatis that are non-pathogenic apparently do not contain these genes.

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Bacterial Blight and Dieback of Eucalyptus Species, Hybrids, and Clones in South Africa

Cited by 80 publications

References 20 publications

Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.

Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.

Enfermedades bacterianas de eucaliptos: Estado actual del conocimiento

Investigation of a sugar N‐formyltransferase from the plant pathogen Pantoea ananatis

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