Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients

Abstract

Salmonella is one of the most common causes of gastroenteritis. In this study, a PCR assay was developed for the rapid detection of microbial cultures of Salmonella enterica sub species of enterica serovar Typhi in samples of patients suspected typhoid. The assay was based on duplicate the STY4669-hypothetical protein gene. The gene primers were designed and blast. Then the bacteria strains were employed for the green synthesis of gold nanoparticles (AuNPs), and one of the strains represented ability to extracellular synthesis of gold nanoparticles. The nanoparticles were characterized using UV-visible spectrophotometer, X-ray powder diffraction, Transmission electron microscopy. The synthesized nanoparticles had a maximum absorption in UV-vis spectra at 556 nm, a crystalline structure, and an average size of 42 nm.

Anti-biofilm effects of gold and silver nanoparticles synthesized by the Rhodiola rosea rhizome extracts

“…The synthesis of nanoparticles was confirmed by a wavelength scan (300-700 nm) of the nanoparticles suspension. From previous studies, it has been suggested that the spectra in the 500-600 nm range correspond to AuNPs and in 350-500 nm range corresponds to AgNPs [34,35]. Both, AuNPs and AgNPs were scanned directly in suspension and with purified nanoparticles (Figure 1(A-D)) [18].…”

Waste-grass-mediated green synthesis of silver nanoparticles and evaluation of their anticancer, antifungal and antibacterial activity

“…During the synthesis of nanoparticles biomolecule, microbial biomass, fungi, plants or their derivatives (biosynthesis) act as both reducing and stabilizing agents of synthesized nanoparticles (33)(34)(35). These reactions can be considered as a green chemistry process that can minimize the environmental hazards in the chemical synthesis processes (36,37).…”

“…. Biosafety issues should be considered in microbiological work as well as large-scale nanoparticle microorganisms and in commercial applications (33). .…”

Nickel-doped cerium oxide nanoparticles: biosynthesis, cytotoxicity and UV protection studies

“…17 The biosynthesis of nanoparticles using natural resources is a modern method with a lower expense and reduced pollution compared to previous methods. [18][19][20][21][22][23][24][25][26][27][28] Salvadora Persica (Salvadoraceae) is a tree with 6-7 m height, and its bark is cracked and wrinkled. It is an indigenous tree of the Middle East, Asia and Africa that commonly grows on the path of ooding and river bank.…”