Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating

Subbiahdoss, Guruprakash; Pidhatika, Bidhari; Coullerez, Géraldine; Charnley, Mirren; Kuijer, Roel; Mei, Henny C. van der; Textor, Marcus; Busscher, Henk J.

doi:10.22203/ecm.v019a20

Cited by 64 publications

(57 citation statements)

References 32 publications

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“…Several biomaterial surface modifications [5–7] have been developed to reduce biofilm formation, but hitherto microbial adhesion can only be reduced by one or two log units and not fully eliminated. Thus long-term treatment of biofilms causing BAI remains a challenge [6].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Gold and Iron-Oxide Nanoparticles on Biofilm-Forming Pathogens

Sathyanarayanan¹,

Balachandranath²,

Srinivasulu³

et al. 2013

ISRN Microbiology

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Microbial biofilms on biomaterial implants or devices are hard to eliminate by antibiotics due to their protection by exopolymeric substances that embed the organisms in a matrix, impenetrable for most antibiotics and immune-cells. Application of metals in their nanoparticulated form is currently considered to resolve bacterial infections. Gold and iron-oxide nanoparticles are widely used in different medical applications, but their utilisation to eradicate biofilms on biomaterials implants is novel. Here, we studied the effect of gold and iron oxide nanoparticles on Staphylococcus aureus and Pseudomonas aeruginosa biofilms. We report that biofilm growth was reduced at higher concentrations of gold and iron-oxide nanoparticles compared to absence of nanoparticles. Thus nanoparticles with appropriate concentration could show significant reduction in biofilm formation.

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Section: Introductionmentioning

confidence: 99%

The Effect of Gold and Iron-Oxide Nanoparticles on Biofilm-Forming Pathogens

Sathyanarayanan¹,

Balachandranath²,

Srinivasulu³

et al. 2013

ISRN Microbiology

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“…However, it would also be of interest to conduct the same study with a longer time of incubation, as has been performed in other studies. 12,13,31 …”

Section: Discussionmentioning

confidence: 99%

Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model

Martínez-Pérez

Pérez‐Jorge

Lozano

et al. 2017

Bone & Joint Research

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ObjectivesImplant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the in vitro studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the ‘race for the surface’ theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied.MethodsWe describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis.ResultsOur results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria.ConclusionsThe method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.Cite this article: M. Martinez-Perez, C. Perez-Jorge, D. Lozano, S. Portal-Nuñez, R. Perez-Tanoira, A. Conde, M. A. Arenas, J. M. Hernandez-Lopez, J. J. de Damborenea, E. Gomez-Barrena, P. Esbrit, J. Esteban. Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the “race for the surface” theory. Bone Joint Res 2017;6:315–322. DOI: 10.1302/2046-3758.65.BJR-2016-0226.R2.

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“…If bacterial cells win the race, infection and ultimately biofilm formation will occur. 15,16 Removal of the implant is a) Author to whom correspondence should be addressed; electronic mail: mukai.cg@mac.com often the only effective method for eradication of infection once a biofilm has formed. This is due to the increased antibiotic resistance of bacteria within biofilms.…”

Section: Introductionmentioning

confidence: 99%

“…This is due to the increased antibiotic resistance of bacteria within biofilms. 16,17 A number of strategies have been investigated to increase soft tissue integration. Hydroxyapatite (HA) has been used to coat the skin-implant interface as it increases in vitro fibroblast adhesion and in vivo soft tissue ingrowth.…”

Section: Introductionmentioning

confidence: 99%

Preventing infection of osseointegrated transcutaneous implants: Incorporation of silver into preconditioned fibronectin-functionalized hydroxyapatite coatings suppresses Staphylococcus aureus colonization while promoting viable fibroblast growth in vitro

Chimutengwende-Gordon

Pendegrass

Bayston

et al. 2014

Biointerphases

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The success of transcutaneous implants depends on the achievement of a soft tissue seal by enabling fibroblasts to win the race for the surface against bacteria. Fibronectin-functionalized hydroxyapatite coatings (HAFn) have been shown to improve dermal tissue ingrowth and attachment. However, during the early postoperative period before a soft tissue seal has formed, bacterial colonization may occur. This study explored the incorporation of silver, a broad spectrum antimicrobial agent, into HAFn coatings with the aim of reducing bacterial colonization. Silver is known to have dose-dependent cytotoxic effects. Therefore, the effects of silver incorporation into HAFn coatings on both in vitro human dermal fibroblast viability and Staphylococcus aureus colonization were assessed. An electrochemical deposition technique was used to codeposit hydroxyapatite and silver (HAAg) and fibronectin was adsorbed onto this to produce HAAgFn coatings. Surfaces were preconditioned with serum to mimic the in vivo environment. Nonpreconditioned HAAg and HAAgFn coatings suppressed bacterial colonization but were cytotoxic. After serum-preconditioning, more than 90% of fibroblasts that grew on all HAAg and HAAgFn coatings were viable. The highest silver content coatings tested (HAAg100 and HAAgFn100) resulted in a greater than 99% reduction in biofilm and planktonic bacterial numbers compared to HA and HAFn controls. Although HAAg100 had greater antibacterial activity than HAAgFn100, the findings of this study indicate that fibroblasts would win the race for the surface against S aureus on both HAAg100 and HAAgFn100 after serum-preconditioning.

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Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating

Cited by 64 publications

References 32 publications

The Effect of Gold and Iron-Oxide Nanoparticles on Biofilm-Forming Pathogens

The Effect of Gold and Iron-Oxide Nanoparticles on Biofilm-Forming Pathogens

Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model

Preventing infection of osseointegrated transcutaneous implants: Incorporation of silver into preconditioned fibronectin-functionalized hydroxyapatite coatings suppresses Staphylococcus aureus colonization while promoting viable fibroblast growth in vitro

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