Bacterial Arsenic Metabolism and Its Role in Arsenic Bioremediation

Kabiraj, Ashutosh; Biswas, Raju; Halder, Urmi; Bandopadhyay, Rajib

doi:10.1007/s00284-022-02810-y

Cited by 33 publications

(8 citation statements)

References 95 publications

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“…This fact should be considered for the bioremediation of wastes co-contaminated with both toxics. Regarding As(V), this bacterium showed MIC values of 1.25 M (with cyanide as N-source) or 1.75 M (with ammonium), and MBC values of 2 M (with cyanide) or 2.5 M (with ammonium), in accordance with the lower toxicity of arsenate compared to arsenite described by other authors [ 48 , 49 , 50 ].…”

Section: Resultssupporting

confidence: 88%

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Biełło

Cabello

Rodríguez-Caballero

et al. 2023

IJMS

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Wastewater from mining and other industries usually contains arsenic and cyanide, two highly toxic pollutants, thereby creating the need to develop bioremediation strategies. Here, molecular mechanisms triggered by the simultaneous presence of cyanide and arsenite were analyzed by quantitative proteomics, complemented with qRT-PCR analysis and determination of analytes in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes CECT 5344. Several proteins encoded by two ars gene clusters and other Ars-related proteins were up-regulated by arsenite, even during cyanide assimilation. Although some proteins encoded by the cio gene cluster responsible for cyanide-insensitive respiration decreased in the presence of arsenite, the nitrilase NitC required for cyanide assimilation was unaffected, thus allowing bacterial growth with cyanide and arsenic. Two complementary As-resistance mechanisms were developed in this bacterium, the extrusion of As(III) and its extracellular sequestration in biofilm, whose synthesis increased in the presence of arsenite, and the formation of organoarsenicals such as arseno-phosphoglycerate and methyl-As. Tetrahydrofolate metabolism was also stimulated by arsenite. In addition, the ArsH2 protein increased in the presence of arsenite or cyanide, suggesting its role in the protection from oxidative stress caused by both toxics. These results could be useful for the development of bioremediation strategies for industrial wastes co-contaminated with cyanide and arsenic.

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Section: Resultssupporting

confidence: 88%

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Biełło

Cabello

Rodríguez-Caballero

et al. 2023

IJMS

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“…wall, plasma membrane or capsule, metal extrusion through efflux pumps, intracellular, periplasmic or extracellular sequestration, precipitation, biosorption and metal biotransformation (Bazzi et al, 2020;Choudhury & Srivastava, 2001;Kabiraj et al, 2022). Several bacteria also use some of these mechanisms to tolerate radionuclides (López-Fernández et al, 2021).…”

Section: Bacteri Al Resistance and Detoxification Of Industri Al Cyan...mentioning

confidence: 99%

“…On the contrary, considering that metals are not degraded easily by microorganisms, resistance mechanisms are mainly based on metal detoxification and/or immobilization. Different types of resistance mechanisms to metals have been described in bacteria (Figure 1 ), including extracellular metal barriers like cell wall, plasma membrane or capsule, metal extrusion through efflux pumps, intracellular, periplasmic or extracellular sequestration, precipitation, biosorption and metal biotransformation (Bazzi et al., 2020 ; Choudhury & Srivastava, 2001 ; Kabiraj et al., 2022 ). Several bacteria also use some of these mechanisms to tolerate radionuclides (López‐Fernández et al., 2021 ).…”

Section: Bacterial Resistance and Detoxification Of Industrial Cyanid...mentioning

confidence: 99%

“…Arsenic is an abundant natural metalloid that can be found in different oxidation states ranging from −3 (arsine), 0 (arsenic), +3 (arsenite) to +5 (arsenate). All these forms of arsenic display different mobility and toxicity, although it is usually found in the environment as arsenate or arsenite (Kabiraj et al., 2022 ; Raju, 2022 ). Inorganic forms of arsenic are present in nature as sulphur‐containing minerals like arsenopyrite (FeAsS), but organic arsenical compounds like methylarsenates are also found.…”

Section: Bacterial Resistance and Detoxification Of Industrial Cyanid...mentioning

confidence: 99%

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Bacterial tolerance and detoxification of cyanide, arsenic and heavy metals: Holistic approaches applied to bioremediation of industrial complex wastes

Olaya‐Abril,

Biełło,

Rodríguez‐Caballero

et al. 2024

Microbial Biotechnology

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Cyanide is a highly toxic compound that is found in wastewaters generated from different industrial activities, such as mining or jewellery. These residues usually contain high concentrations of other toxic pollutants like arsenic and heavy metals that may form different complexes with cyanide. To develop bioremediation strategies, it is necessary to know the metabolic processes involved in the tolerance and detoxification of these pollutants, but most of the current studies are focused on the characterization of the microbial responses to each one of these environmental hazards individually, and the effect of co‐contaminated wastes on microbial metabolism has been hardly addressed. This work summarizes the main strategies developed by bacteria to alleviate the effects of cyanide, arsenic and heavy metals, analysing interactions among these toxic chemicals. Additionally, it is discussed the role of systems biology and synthetic biology as tools for the development of bioremediation strategies of complex industrial wastes and co‐contaminated sites, emphasizing the importance and progress derived from meta‐omic studies.

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“…They can, however, help us to understand some of the key processes that “animate” chemical biology, notably by tracking isotope enrichment in mixtures of isotopes of a given atom (Danchin, 2020). More radical changes would use atoms avoided by life but sometimes used, such as fluorine and other halogens (Alves Ferreira‐Bravo & DeStefano, 2021; Heine et al, 2018), silicon (Ikeda, 2021) or arsenic (Kabiraj et al, 2022). The narrow scope of the reactivity of these atoms is such that, when used in biological processes, they are and will remain confined to a limited set of metabolic pathways, which can nevertheless be expected to attract attention in the coming years.…”

Section: From the Proof Of Concept To The Construction Of Living Cellsmentioning

confidence: 99%

SynBio 2.0, a new era for synthetic life: Neglected essential functions for resilience

Danchin

Huang

2022

Environmental Microbiology

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Bacterial Arsenic Metabolism and Its Role in Arsenic Bioremediation

Cited by 33 publications

References 95 publications

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Bacterial tolerance and detoxification of cyanide, arsenic and heavy metals: Holistic approaches applied to bioremediation of industrial complex wastes

SynBio 2.0, a new era for synthetic life: Neglected essential functions for resilience

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