Bacterial Alpha Amylase Paper Disc Tests on Starch Agar

Abstract

TILDEN, E. B., AND HUDSON, C. S. 1942 Preparation and properties of the amylases produced by Bacillus macerans and Bacillus polymyza. J. Bacteriol., 43, 527-544. WAKSMAN, S. A. 1919 Cultural studies of species of actinomyces. Soil Sci., 8, 71-215. WAK5MAN, S. A. 1950 The Actinomycetes. Chronica Botanica Company, Waltham, Mass.

Bacillus stearothermophilus

“…Disc-plate test. The procedure was that of Wellerson et al (1952Wellerson et al ( , 1954 and Stark et al (1953). Filter-paper discs, saturated with the amylase solution, were placed on the surface of a starch-agar substrate in pressed Pyrex Petri plates (4 discs/plate).…”

Differential Heat Stabilities of Bacillus Amylases

“…Growth was observed visually on plates or turbidimetrically in broth cultures grown in Nephelo culture flasks (Bellco). Amylase activity was determined by flooding the culture plates with a dilute iodine solution (12). Broth culture supernatant fluids were examined by using disc-plate (4, 12), blue-value reduction (11) and (or) automated reducing-value (procedure B of reference 10) methods.…”

Amylolytic enzymes and selected physiological properties of Streptococcus bovis and Streptococcus equinus

“…Maybe the cause '•' of this is the difference in viscosity of the concentrated and dilute enzyme solutions, because the concentrated solutions were absorbed into the discs slowly compared to the dilute solutions. It might have been better to pipette 0.07 ml/disc rather than dip the disc (Stark et aj.., 1953) • Over a certain range, the amount of enzyme per disc, the concentration of the starch and the concentration of the amylase, respectively, are linearly related to the diameter of the zone of hydrolysis (Stark et al, 1953).…”

“…The method of Stark et (1953) was used for paper disc tests on starch agar. The assay medium contained 0.066 M potassium phosphate buffer at pK 6.0; 0.2^ soluble starch powder (Mallinckrodt); 1.0^ agar (Difco); and 0.09% NaCl.…”