Bacterial adhesion to biological versus polymer prosthetic materials used in abdominal wall defect repair: do these meshes show any differences in vitro?

Pérez-Köhler, Bárbara; Sotomayor, Sandra; Rodrı́guez, Marta; Gegúndez, María Isabel; Pascual, Gemma; Bellón, Juan M.

doi:10.1007/s10029-015-1378-1

Cited by 16 publications

(17 citation statements)

References 30 publications

(44 reference statements)

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“…As regards hernia prostheses, even if mesh infection is relatively rare, the most common bacteria adhering to them are Staphylococcus aureus and Staphylococcus epidermidis . This fact was mainly demonstrated by clinical studies and “ in vitro ” experiments, also showing that bacterial adhesion is affected by prosthesis chemical composition and morphology …”

Section: Discussionmentioning

confidence: 93%

“…This fact was mainly demonstrated by clinical studies and "in vitro" experiments, also showing that bacterial adhesion is affected by prosthesis chemical composition and morphology. [41][42][43]48,49 More recently, Ag nanoparticles have also been shown to inhibit, to varying extents, the growth and viability of certain microorganisms, possibly through the production of free radicals inducing membrane damage, or via the release of Ag 1 ions. 24,50 The mesh layer of CMC for hernia repair was thus coated with Ag/SiO 2 nanoclusters, in order to confer antibacterial properties without altering biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

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Biocompatibility versus peritoneal mesothelial cells of polypropylene prostheses for hernia repair, coated with a thin silica/silver layer

et al. 2016

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Hernias are generally repaired using synthetic prostheses. Infection may already be present or develop during implantation. Based on the increasing resistance to antibiotics, and the well-known antimicrobial properties of silver (Ag), the possibility of coating hernia prostheses with a nanostructured layer containing Ag was explored. Prostheses (Clear Mesh Composite [CMC]) made up of two polypropylene layers (macroporous light mesh and thin transparent film) were tested with human mesothelial cells from omentum biopsies. Mesotheliocytes modulate abdominal wall healing producing cytokines, growth factors, and adhesion molecules. Evaluating the growth of these cells on CMC or film alone showed that cell numbers on CMC increased over time, and were higher than those on film alone. Vimentin immunostaining confirmed the cells to be mesotheliocytes. Subsequently, the biocompatibility of mesh layer, coated or not with a thin layer of Ag/SiO -nanoclusters, was analyzed, showing no difference in absence or presence of Ag/SiO . Differently, TGF-β2 production, involved in tissue repair and fibrosis, increased in the presence of Ag/SiO . Moreover, Ag/SiO -coated mesh showed antibacterial properties. In conclusion, the mesh layer coated with Ag/SiO afforded cell growth, and showed antibacterial activity. Coating only the mesh layer did not decrease film transparency, and did not favor the formation of adhesions on the visceral side. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1586-1593, 2017.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Biocompatibility versus peritoneal mesothelial cells of polypropylene prostheses for hernia repair, coated with a thin silica/silver layer

et al. 2016

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“…Biologically derived bioprosthetics were expected to at least partially overcome these issues, due to their biological origin and ability to be remodeled and revascularized. However, this remains a controversial point as recent studies have demonstrated that biologically derived prosthetics may also be susceptible to the negative effects of bacterial colonization, resulting in prolonged colonization and premature degradation [8, 16–18]. Studies have also shown that some biologically derived prosthetics exhibit inconsistent properties due to variation in donor tissue characteristics and quality control [19].…”

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confidence: 99%

Evaluation of a fully absorbable poly-4-hydroxybutyrate/absorbable barrier composite mesh in a porcine model of ventral hernia repair

et al. 2016

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BackgroundThe objective of this study was to evaluate the mechanical and histological properties of a fully absorbable poly-4-hydroxybutyrate/absorbable barrier composite mesh (Phasix™ ST) compared to partially absorbable (Ventralight™ ST), fully absorbable (Phasix™), and biologically derived (Strattice™) meshes in a porcine model of ventral hernia repair.MethodsBilateral abdominal surgical defects were created in twenty-four Yucatan pigs, repaired with intraperitoneal (Phasix™ ST, Ventralight™ ST) or retromuscular (Phasix™, Strattice™) mesh, and evaluated at 12 and 24 weeks (n = 6 mesh/group/time point).ResultsPrior to implantation, Strattice™ demonstrated significantly higher (p < 0.001) strength (636.6 ± 192.1 N) compared to Ventralight™ ST (324.3 ± 37.1 N), Phasix™ ST (206.9 ± 11.3 N), and Phasix™ (200.6 ± 25.2 N). At 12 and 24 weeks, mesh/repair strength was significantly greater than NAW (p < 0.01 in all cases), and no significant changes in strength were observed for any meshes between 12 and 24 weeks (p > 0.05). Phasix™ mesh/repair strength was significantly greater than Strattice™ (p < 0.001) at 12 and 24 weeks, and Ventralight™ ST mesh/repair strength was significantly greater than Phasix™ ST mesh (p < 0.05) at 24 weeks. At 12 and 24 weeks, Phasix™ ST and Ventralight™ ST were associated with mild inflammation and minimal–mild fibrosis/neovascularization, with no significant differences between groups. At both time points, Phasix™ was associated with minimal–mild inflammation/fibrosis and mild neovascularization. Strattice™ was associated with minimal inflammation/fibrosis, with minimal neovascularization at 12 weeks, which increased to mild by 24 weeks. Strattice™ exhibited significantly less neovascularization than Phasix™ at 12 weeks and significantly greater inflammation at 24 weeks due to remodeling.ConclusionsPhasix™ ST demonstrated mechanical and histological properties comparable to partially absorbable (Ventralight™ ST) and fully resorbable (Phasix™) meshes at 12 and 24 weeks in this model. Data also suggest that fully absorbable meshes with longer-term resorption profiles may provide improved mechanical and histological properties compared to biologically derived scaffolds.

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“…A previous in vitro study has shown that Surgisis ® has a higher bacterial load than other polymer materials, when incubated with Staphylococcus [22], which might indicate that it should be avoided in contaminated surgical fields. Similarly, a clinical study on hernia repair has indicated that the frequency of wound infections could be high when Surgisis ® is used [14].…”

Section: Discussionmentioning

confidence: 95%

Complications, long-term outcome and quality of life following Surgisis® and muscle-covered implants in immediate breast reconstruction: a case-control study with a 6-year follow-up

et al. 2018

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Background Matrices are now commonly used in breast reconstruction, but the scientific evidence is still scares. The main aim was to compare complications and the need for corrections in immediate breast reconstruction with the porcine-derived Surgisis ® with the traditional muscle-covered technique. The secondary aim was to compare long-term quality of life and satisfaction. Methods All consecutive patients who had their breast reconstructed with a Surgisis ® or muscle-covered tissue expander/implant were included. Patients were followed clinically and with BREAST-Q. Results During the study period, 116 reconstructions (71 patients) were operated in the Surgisis ® group and 132 reconstructions (90 patients) in the control group. The median follow-up time was 74 months (min 43-max 162). The total early complication rate was 37% in the Surgisis ® group and 27% in the control group. There were no differences in implant loss (p = 0.68) or total number of complications (p = 0.24) between the two groups. Risk factors for complications were mainly patient characteristics and the use of a tissue expander. There was a slightly higher capsular contracture frequency in the Surgisis ® patients (4.2% vs. 2.5%). The need for corrections and patient satisfaction and quality of life were similar in the two groups. Conclusions The use of Surgisis ® in implant-based reconstruction seems to result in an acceptable total early complication rate. The rate might be higher in tissue expander-based reconstruction. Risk factors are mainly patient characteristics. The capsular contracture rate and need for corrections, as well as patient satisfaction and quality of life, are similar in the Surgisis ® patients and muscle-covered controls. Level of evidence: III

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Bacterial adhesion to biological versus polymer prosthetic materials used in abdominal wall defect repair: do these meshes show any differences in vitro?

Abstract: Within 48 h of contamination, the absorbable polymer and biological meshes exhibited high bacterial loads. Given their lower affinity for both bacterial strains, the conventional non-absorbable polymer materials could be better candidates for use in contaminated surgical fields.

Cited by 16 publications

References 30 publications

Biocompatibility versus peritoneal mesothelial cells of polypropylene prostheses for hernia repair, coated with a thin silica/silver layer

Biocompatibility versus peritoneal mesothelial cells of polypropylene prostheses for hernia repair, coated with a thin silica/silver layer

Evaluation of a fully absorbable poly-4-hydroxybutyrate/absorbable barrier composite mesh in a porcine model of ventral hernia repair

Complications, long-term outcome and quality of life following Surgisis® and muscle-covered implants in immediate breast reconstruction: a case-control study with a 6-year follow-up

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