Bacterial adhesion on orthopedic implants

Filipović, Urška; Dahmane, Raja; Ghannouchi, Sonia Ayachi; Zore, Anamarija; Bohinc, Klemen

doi:10.1016/j.cis.2020.102228

Cited by 152 publications

(116 citation statements)

References 80 publications

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“…Compared with the surrounding aqueous environment, bacteria prefer to adhere to the surface of the material. The first stage of bacterial adhesion on the implant surface involves the initial, non-specific, reversible physical and chemical attachment, whereas the second stage involves the specific, irreversible molecular and cell attachment (Filipovic et al, 2020).…”

Section: Bacterial Adhesionmentioning

confidence: 99%

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Recent Advances in Research on Antibacterial Metals and Alloys as Implant Materials

Jiao

Zhang

et al. 2021

Front. Cell. Infect. Microbiol.

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Implants are widely used in orthopedic surgery and are gaining attention of late. However, their use is restricted by implant-associated infections (IAI), which represent one of the most serious and dangerous complications of implant surgeries. Various strategies have been developed to prevent and treat IAI, among which the closest to clinical translation is designing metal materials with antibacterial functions by alloying methods based on existing materials, including titanium, cobalt, tantalum, and biodegradable metals. This review first discusses the complex interaction between bacteria, host cells, and materials in IAI and the mechanisms underlying the antibacterial effects of biomedical metals and alloys. Then, their applications for the prevention and treatment of IAI are highlighted. Finally, new insights into their clinical translation are provided. This review also provides suggestions for further development of antibacterial metals and alloys.

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Section: Bacterial Adhesionmentioning

confidence: 99%

“…Adhesin-mediated interactions cause bacteria to aggregate. Bacterial adhesion is a complex process that is affected by bacterial characteristics, material surface characteristics, environmental factors, and hydrodynamic factors (Filipovic et al, 2020).…”

Section: Bacterial Adhesionmentioning

confidence: 99%

Recent Advances in Research on Antibacterial Metals and Alloys as Implant Materials

Jiao

Zhang

et al. 2021

Front. Cell. Infect. Microbiol.

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“…In this regard, bone implants require a long list of properties and functionalities to serve their purpose most properly. In addition to primary stability [ 1,2 ] and bone‐matching mechanical properties, [ 3 ] bone implants need to be designed in such a way to promote host tissue regeneration and integration with the implant (i.e., osteoinduction and osseointegration), [ 4–6 ] as well as to combat implant‐associated infections (IAIs), [ 7–9 ] as one of the main causes of implant failure.…”

Section: Introductionmentioning

confidence: 99%

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

Modaresifar

Ganjian

Angeloni

et al. 2021

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Despite the potential of small‐scale pillars of black titanium (bTi) for killing the bacteria and directing the fate of stem cells, not much is known about the effects of the pillars’ design parameters on their biological properties. Here, three distinct bTi surfaces are designed and fabricated through dry etching of the titanium, each featuring different pillar designs. The interactions of the surfaces with MC3T3‐E1 preosteoblast cells and Staphylococcus aureus bacteria are then investigated. Pillars with different heights and spatial organizations differently influence the morphological characteristics of the cells, including their spreading area, aspect ratio, nucleus area, and cytoskeletal organization. The preferential formation of focal adhesions (FAs) and their size variations also depend on the type of topography. When the pillars are neither fully separated nor extremely tall, the colocalization of actin fibers and FAs as well as an enhanced matrix mineralization are observed. However, the killing efficiency of these pillars against the bacteria is not as high as that of fully separated and tall pillars. This study provides a new perspective on the dual‐functionality of bTi surfaces and elucidates how the surface design and fabrication parameters can be used to achieve a surface topography with balanced bactericidal and osteogenic properties.

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“…Detachment and dispersion, whereby bacterial cells are released from the biofilm ( Costerton, 1995 ; Costerton et al, 1995 ). Bacterial attachment to different surfaces is regulated by the interplay between multiple biological factors as well as the bacterial response to surface chemistry and topography ( Filipovic et al, 2020 ). The extracellular matrix is the “hallmark” of bacterial biofilms and accounts for 85% of the overall biofilm volume as compared to the cells constituting only 15% of the biofilm by volume ( Donlan and Costerton, 2002 ; Flemming and Wingender, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Biofilm Formation by Mycobacterium chimaera on Medical Device Materials

et al. 2021

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Non-tuberculous mycobacteria (NTM) are widespread in the environment and are a public health concern due to their resistance to antimicrobial agents. The colonization of surgical heater-cooler devices (HCDs) by the slow-growing NTM species Mycobacterium chimaera has recently been linked to multiple invasive infections in patients worldwide. The resistance of M. chimaera to antimicrobials may be aided by a protective biofilm matrix of extracellular polymeric substances (EPS). This study explored the hypothesis that M. chimaera can form biofilms on medically relevant materials. Several M. chimaera strains, including two HCD isolates, were used to inoculate a panel of medical device materials. M. chimaera colonization of the surfaces was monitored for 6 weeks. M. chimaera formed a robust biofilm at the air-liquid interface of borosilicate glass tubes, which increased in mass over time. M. chimaera was observed by 3D Laser Scanning Microscopy to have motility during colonization, and form biofilms on stainless steel, titanium, silicone and polystyrene surfaces during the first week of inoculation. Scanning electron microscopy (SEM) of M. chimaera biofilms after 4 weeks of inoculation showed that M. chimaera cells were enclosed entirely in extracellular material, while cryo-preserved SEM samples further revealed that an ultrastructural component of the EPS matrix was a tangled mesh of 3D fiber-like projections connecting cells. Considering that slow-growing M. chimaera typically has culture times on the order of weeks, the microscopically observed ability to rapidly colonize stainless steel and titanium surfaces in as little as 24 h after inoculation is uncharacteristic. The insights that this study provides into M. chimaera colonization and biofilm formation of medical device materials are a significant advance in our fundamental understanding of M. chimaera surface interactions and have important implications for research into novel antimicrobial materials, designs and other approaches to help reduce the risk of infection.

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Bacterial adhesion on orthopedic implants

Cited by 152 publications

References 80 publications

Recent Advances in Research on Antibacterial Metals and Alloys as Implant Materials

Recent Advances in Research on Antibacterial Metals and Alloys as Implant Materials

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

Characterization of Biofilm Formation by Mycobacterium chimaera on Medical Device Materials

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