Bacteria-Responsive Biomimetic Selenium Nanosystem for Multidrug-Resistant Bacterial Infection Detection and Inhibition

Abstract

Multidrug-resistant (MDR) bacterial infections are a severe threat to public health owing to their high risk of fatality. Noticeably, the premature degradation and undeveloped imaging ability of antibiotics still remain challenging. Herein, a selenium nanosystem in response to a bacteria-infected microenvironment is proposed as an antibiotic substitute to detect and inhibit methicillin-resistant Staphylococcus aureus (MRSA) with a combined strategy. Using natural red blood cell membrane (RBCM) and bacteria-responsive gelatin nanoparticles (GNPs), the Ru–Se@GNP-RBCM nanosystem was constructed for effective delivery of Ru-complex-modified selenium nanoparticles (Ru–Se NPs). Taking advantage of natural RBCM, the immune system clearance was reduced and exotoxins were neutralized efficiently. GNPs could be degraded by gelatinase in pathogen-infected areas in situ; therefore, Ru–Se NPs were released to destroy the bacteria cells. Ru–Se NPs with intense fluorescence imaging capability could accurately monitor the infection treatment process. Moreover, excellent in vivo bacteria elimination and a facilitated wound healing process were confirmed by two kinds of MRSA-infected mice models. Overall, the above advantages proved that the prepared nanosystem is a promising antibiotic alternative to combat the ever-threatening multidrug-resistant bacteria.

Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy

“…It was speculated that PTR-701Ns were cleared by macrophages in vivo. But with the coating of RBCM, RPTR-701Ns maintained a longer circulation in vivo, so as to promote the aggregation of the drug at infected site and achieve a better therapeutic effect [36].…”

“…However, the synthesis of the nanomaterial was quite complicated and the safety of the carrier was unknown. Ange Lin et al [36] designed RBCM coated gelatin nanoparticles loaded Ru−Se (Ru−Se @GNP-RBCM), in which each part of the bacteriaresponsive nano-system possessed a synergistic effect in bacterial infection treatment. This research was relatively complete, but the preparation of Ru-Se was time-consuming and relevant characterization was needed to determine the success of the synthesis.…”

Colloidal Surface Engineering: Growth of Layered Double Hydroxides with Intrinsic Oxidase‐Mimicking Activities to Fight Against Bacterial Infection in Wound Healing

“…To further determine the role of nanozymes in antagonizing infection in vivo, mice wound healing model (8 mm in diameter) with local infection of S. aureus was established as previously reported with minor modification. [55] Each group of mice (n = 8 in each group) was treated with phosphate buffer saline (PBS), SiO 2 nanoparticle, hollow Mn/Ni(OH) x LDHs, and SiO 2 nanorods@Mn/Ni(OH) x LDHs, respectively, and the photos of wound were taken on every other day postincision to analyze the degree of wound contraction (Figure 6a). Negative "blank" control group was also established in WT mice, in which the wound was made without infection of S. aureus, and wound healing process was observed for 14 days ( Figure S22, Supporting Information).…”

Dual Antibacterial Effect Of In-situ Electrospun Curcumin Composite Nanofibers To Sterilize Drug-resistant Bacteria

“…Bacterial infection without timely treatment will cause septicemia and sepsis thus seriously endanger life and health [1][2][3]. Although antibiotic can kill bacteria, using antibiotic in long term will lead to the development of drug-resistant bacteria, such as methicillin-resistant staphylococcus aureus (MRSA) [4][5][6].…”