Bacteria-derived fluorescent carbon dots for highly selective detection of<i>p</i>-nitrophenol and bioimaging

Zhang, Shengting; Zhang, Dongfang; Ding, Yafang; Hua, Jianhao; Tang, Bing; Ji, Xiuling; Zhāng, Qí; Wei, Yunlin; Qin, Kunhao; Li, Bo

doi:10.1039/c9an01103j

Cited by 69 publications

(18 citation statements)

References 42 publications

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“…Recently, carbon dots have interested the research community as bioimaging labels and sensors due to their high performances, including bright fluorescence (Zhu et al, 2013), excellent photostability (Zhang et al, 2019), tunable fluorescence emission (Sun et al, 2006), favorable biocompatibility (Deng et al, 2019), low toxicity (Ye et al, 2019), and good water solubility (Gao Y. et al, 2019). As presented in the available documents, most of carbon dots showed great potential in bioimaging of different types of cells.…”

Section: Introductionmentioning

confidence: 99%

Fe3+-Sensitive Carbon Dots for Detection of Fe3+ in Aqueous Solution and Intracellular Imaging of Fe3+ Inside Fungal Cells

et al. 2020

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In this article, the Fe 3+-sensitive carbon dots were obtained by means of a microwave-assisted method using glutamic acid and ethylenediamine as reactants. The carbon dots exhibited selective response to Fe 3+ ions in aqueous solution with a turn-off mode, and a good linear relationship was found between (F 0-F)/F 0 and the concentration of Fe 3+ in the range of 8-80 µM. As a result, the as-synthesized carbon dots can be developed as a fluorescent chemosensor for Fe 3+ in aqueous solution. Moreover, the carbon dots can be applied as a fluorescent agent for fungal bioimaging since the fungal cells stained by the carbon dots were brightly illuminated on a confocal microscopy excited at 405 nm. Furthermore, an increase in the concentration of intracellular Fe 3+ could result in fluorescence quenching of the carbon dots in the fungal cells when incubated in the Tris-HCl buffer solution containing Fe 3+. However, due to EDTA might hinder Fe(III) to enter the fungal cells, incubation in Fe(III)-EDTA complex solution exerted negligible effect on the fluorescence of fungal cells labeled by the carbon dots. Therefore, the carbon dots can serve as a potential probe for intracellular imaging of Fe 3+ inside fungal cells.

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Section: Introductionmentioning

confidence: 99%

Fe3+-Sensitive Carbon Dots for Detection of Fe3+ in Aqueous Solution and Intracellular Imaging of Fe3+ Inside Fungal Cells

et al. 2020

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“…Relative to quinine sulfate, quantum yields of B-C-dots and E-C-dots were found to be 4.0% and 5.0%, respectively (Figure S2). These quantum yields are relatively low compared with quantum yields from other bacterially-derived C-dots carbonized using the same method [20][21][22]. However, quantum yields in the literature are obtained in water, while we preferred to measure quantum yields under more physiological conditions, i.e., in a 10 mM phosphate buffer (pH 7.4).…”

Section: Physico-chemical Characterization Of Hydrothermally Derived C-dots and Their Source Bacteriamentioning

confidence: 99%

“…C-dots possess diameters less than 10 nm and have good photostability combined with low toxicity [12,13]. C-dots are easily synthesized from a wide variety of carbon sources, which include spermidine [14], gentamicin sulfate [15], vitamin C [16], carbon nanopowders [17], cigarette smoke [18], polyethyleneimine and citric acid [19] and bacteria [20][21][22][23]. Interestingly, C-dots synthesized from antibiotics, and antimicrobial ammonium salts can inherit chemical functionalities from their carbon sources, yielding C-dots with antibacterial activity against a broad spectrum of Gram-positive and Gramnegative pathogens [15,24,25].…”

Section: Introductionmentioning

confidence: 99%

Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice

Yang

Mei

et al. 2021

Pharmaceutics

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Orally administrated probiotic bacteria can aid antibiotic treatment of intestinal infections, but their arrival at their intestinal target site is hampered by killing in the gastrointestinal tract and by antibiotics solely intended for pathogen killing. Carbon-quantum-dots are extremely small nanoparticles and can be derived from different sources, including bacteria. Here, we hypothesize that carbon-quantum-dots inherit antibacterial activity from probiotic source bacteria to fulfill a similar role as live probiotics in intestinal infection therapy. Physico-chemical analyses indicated that carbon-quantum-dots, hydrothermally derived from Bifidobacterium breve (B-C-dots), inherited proteins and polysaccharides from their source-bacteria. B-C-dots disrupted biofilm matrices of Escherichia coli and Salmonella typhimurium biofilms through extensive reactive-oxygen-species (ROS)-generation, causing a decrease in volumetric bacterial-density in biofilms. Decreased bacterial densities leave more open space in biofilms and have enhanced ciprofloxacin penetration and killing potential in an E. coli biofilm pre-exposed to probiotic B-C-dots. Pathogenic carbon-quantum-dots hydrothermally derived from E. coli (E-C-dots) did not disrupt pathogenic biofilms nor enhance E. coli killing potential by ciprofloxacin. B-C-dots were biosafe in mice upon daily administration, while E-C-dots demonstrated a decrease in white blood cell and platelet counts and an increase in C-reactive protein levels. Therefore, the way is paved for employing probiotic carbon-quantum-dots instead of viable, probiotic bacteria for synergistic use with existing antibiotics in treating intestinal infections.

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“…[ 89 ] To synthesize fluorescent CDs by a single‐step process, B. cereus was used as a carbon source. [ 91 ] Recently, Li et al synthesized two types of luminescent carbon dots with the help of a hydrothermal process in which bacterial cellulose was used as a carbon source. [ 92 ]…”

Section: Microbial Synthesis Of Metal Nanoparticlesmentioning

confidence: 99%

Metal Nanoparticle–Microbe Interactions: Synthesis and Antimicrobial Effects

Khan

Fromm

Rizvi

et al. 2020

Part & Part Syst Charact

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metal solution, reduction of the metal ions leads to the generation of metal atom clustering generating nanosized particles. A characteristic of biosynthetic NPs is their high stability as the organisms provide their own biomolecular capping agent(s). [1] While plant-based production of NPs is now considered a scientific curiosity rather than a promising biotechnology, [2] bacteriamediated NP biosynthesis offers better chances, in light of the poorly characterized microbial diversity and the complex chemistry of enzymes in metal-reducing bacteria. Two recent works focused on directed biofabrication of CdSe-nanoparticles through regulating extracellular electron transfer [3] and the biosynthesis of highly active copper NPs (CuNP) by Shewanella oneidensis. [4] However, the description of molecular mechanism(s) involved in the biosynthesis of NPs has received little attention, which is the focus of this review.The CFCF or culture supernatants (CS) of bacteria mediate the synthesis of AgNPs [5] as presented in this section. The CFCF from the family Enterobacteriaceae reduce silver ions to AgNPs ( Table 1). CFCF of Klebsiella pneumoniae, Escherichia coli, and Metal nanoparticles (NPs), chalcogenides, and carbon quantum dots can be easily synthesized from whole microorganisms (fungi and bacteria) and cell-free sterile filtered spent medium. The particle size distribution and the biosynthesis time can be somewhat controlled through the biomass/metal solution ratio. The biosynthetic mechanism can be explained through the ionreduction theory and UV photoconversion theory. Formation of biosynthetic NPs is part of the detoxification strategy employed by microorganisms, either in planktonic or biofilm form, to reduce the chemical toxicity of metal ions. In fact, most reports on NP biosynthesis show extracellular metal ion reduction. This is important for environmental and industrial applications, particularly in biofilms, as it allows in principle high biosynthetic rates. The antimicrobial and antifungal effect on biosynthetic NPs can be explained in terms of reactive oxygen species and can be enhanced by the capping agents attached to the NP during the biosynthesis process. Industrial applications of NP biosynthesis are still lagging, due to the difficulty of controlling NP size and low titer. Further, the environmental assessment of biosynthetic NPs has not yet been carried out. It is expected that further advancements in biosynthetic NP research will lead to applications, particularly in environmental biotechnology.

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Bacteria-derived fluorescent carbon dots for highly selective detection ofp-nitrophenol and bioimaging

Abstract: Schematic of the synthetic route for fluorescent CDs-BC and their applications in the detection of p-NP and bioimaging.

Cited by 69 publications

References 42 publications

Fe3+-Sensitive Carbon Dots for Detection of Fe3+ in Aqueous Solution and Intracellular Imaging of Fe3+ Inside Fungal Cells

Fe3+-Sensitive Carbon Dots for Detection of Fe3+ in Aqueous Solution and Intracellular Imaging of Fe3+ Inside Fungal Cells

Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice

Metal Nanoparticle–Microbe Interactions: Synthesis and Antimicrobial Effects

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