Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal

Vicente, Cláudia S. L.; Nascimento, Francisco X.; Espada, Margarida; Mota, Manuel; Oliveira, Solange

doi:10.1007/s10482-011-9602-1

Cited by 35 publications

(48 citation statements)

References 18 publications

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“…A total of 98 sequences were found: 60 from Portugal in 2010 [8]; 21 from Portugal in 2011 [26]; 2 from China in 2012 [29] and 15 from China in 2013 [12]. The sequences were aligned and the dendrograms were constructed as mentioned above.…”

Section: Methodsmentioning

confidence: 99%

“…In Republic of Korea, the bacterial genus associated to PWN found in common with China was Serratia [25]. Recently, it was shown that in Portugal bacteria associated with PWN were mainly belonging to the genera Pseudomonas , Burkholderia , and to the family Enterobacteriaceae [8], [26]. Others suggested that bacteria, carried by the PWN, are phytotoxin producers and interact with the nematode.…”

Section: Introductionmentioning

confidence: 99%

“…Some studies characterized their isolates by cultivation methods and identification kits [16], [20]–[25], [28] and other sequenced the 16S rRNA genes and queried them with the international databases [8], [12], [26], [28], [29]. Therefore, identifications of the isolated strains obtained only by biochemical methods may be incomplete, especially for some of the genera, potentially leading to wrong conclusions regarding bacterial communities.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Bacteria Carried by Pinewood Nematode in USA and Phylogenetic Comparison with Isolates from Other Countries

et al. 2014

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Pine wilt disease (PWD) is native to North America and has spread to Asia and Europe. Lately, mutualistic relationship has been suggested between the pinewood nematode (PWN), Bursaphelenchus xylophilus the causal nematode agent of PWD, and bacteria. In countries where PWN occurs, nematodes from diseased trees were reported to carry bacteria from several genera. However no data exists for the United States. The objective of this study was to evaluate the diversity of the bacterial community carried by B. xylophilus, isolated from different Pinus spp. with PWD in Nebraska, United States. The bacteria carried by PWN belonged to Gammaproteobacteria (79.9%), Betaproteobacteria (11.7%), Bacilli (5.0%), Alphaproteobacteria (1.7%) and Flavobacteriia (1.7%). Strains from the genera Chryseobacterium and Pigmentiphaga were found associated with the nematode for the first time. These results were compared to results from similar studies conducted from other countries of three continents in order to assess the diversity of bacteria with associated with PWN. The isolates from the United States, Portugal and China belonged to 25 different genera and only strains from the genus Pseudomonas were found in nematodes from all countries. The strains from China were closely related to P. fluorescens and the strains isolated from Portugal and USA were phylogenetically related to P. mohnii and P. lutea. Nematodes from the different countries are associated with bacteria of different species, not supporting a relationship between PWN with a particular bacterial species. Moreover, the diversity of the bacteria carried by the pinewood nematode seems to be related to the geographic area and the Pinus species. The roles these bacteria play within the pine trees or when associated with the nematodes, might be independent of the presence of the nematode in the tree and only related on the bacteria's relationship with the tree.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of Bacteria Carried by Pinewood Nematode in USA and Phylogenetic Comparison with Isolates from Other Countries

et al. 2014

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“…As complex mixtures, EOs may display several biological activities which makes them desirable biopesticides, being able to control not just the targeted pest but also opportunistic species and resistant strains. This is of particular interest in phytoparasitic nematode control since complex disease symptoms are also commonly associated with accompanying pathogenic microbiota (Back et al, 2002;Vicente et al, 2011). Several EOs, such as those of Cinnamomum zeylanicum (Kong et al, 2006); Boswellia carterii, Paeonia moutan, Perilla frutescens, Schizonepeta tenuifolia (Choi et al, 2007a); Thymus vulgaris (Kong et al, 2007); Litsea cubeba, Pimenta dioica, Trachyspermum ammi ; Coriandrum sativum and Liquidambar orientalis (Kim et al, 2008) have revealed strong activities against B. xylophilus.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity against Bursaphelenchus xylophilus: Nematotoxics from essential oils, essential oils fractions and decoction waters

et al. 2013

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a b s t r a c tThe Portuguese pine forest has become dangerously threatened by pine wilt disease (PWD), caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus. Synthetic chemicals are the most common pesticides used against phytoparasitic nematodes but its use has negative ecological impacts. Phytochemicals may prove to be environmentally friendly alternatives. Essential oils (EOs) and decoction waters, isolated from 84 plant samples, were tested against B. xylophilus, in direct contact assays. Some successful EOs were fractionated and the fractions containing hydrocarbons or oxygen-containing molecules tested separately. Twenty EOs showed corrected mortalities P96% at 2 lL/mL. These were further tested at lower concentrations. Ruta graveolens, Satureja montana and Thymbra capitata EOs showed lethal concentrations (LC 100 ) < 0.4 lL/mL. Oxygen-containing molecules fractions showing corrected mortality P96% did not always show LC 100 values similar to the corresponding EOs, suggesting additive and/or synergistic relationships among fractions. Nine decoction waters (remaining hydrodistillation waters) revealed 100% mortality at a minimum concentration of 12.5 lL/mL. R. graveolens, S. montana and T. capitata EOs are potential environmentally friendly alternatives for B. xylophilus control given their high nematotoxic properties. Nematotoxic activity of an EO should be taken in its entirety, as its different components may contribute, in distinct ways, to the overall EO activity.

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“…In Korea, the genera Burkholderia, Brevibacterium, Ewingella, Enterobacter and Serratia appear to be associated with the PWN (Kwon et al 2010). More recently, in Portugal, the genera Burkholderia and Pseudomonas, as well as other bacteria from the Enterobacteriaceae family, were found to be associated with B. xylophilus (Proença et al 2010;Vicente et al 2011). However, other studies have shown that the PWN is able to cause pathogenicity under aseptic conditions (Tamura 1983;Kuroda and Ito 1992).…”

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confidence: 99%

Pine Wilt Disease: a threat to European forestry

et al. 2011

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Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal

Cited by 35 publications

References 18 publications

Diversity of Bacteria Carried by Pinewood Nematode in USA and Phylogenetic Comparison with Isolates from Other Countries

Diversity of Bacteria Carried by Pinewood Nematode in USA and Phylogenetic Comparison with Isolates from Other Countries

Bioactivity against Bursaphelenchus xylophilus: Nematotoxics from essential oils, essential oils fractions and decoction waters

Pine Wilt Disease: a threat to European forestry

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