Bacteremia and Thrombus on Prosthetic Heart Valves in Dogs

Jones, Richard D.; Akao, Motoichi; Cross, Frederick S.

doi:10.1161/01.cir.39.5s1.i-253

Cited by 6 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Goats have been found to be a good model for valve testing, but their availability is often limited ( 10 ). The relative susceptibility of dogs to sepsis and thrombosis has marked these animals as less than optimal ( 11 ). Sheep ( Ovis aris ) are widely used for testing prosthetic cardiac valves.…”

Section: Discussionmentioning

confidence: 99%

Development of an Animal Experimental Model for a Bileaflet Mechanical Heart Valve Prosthesis

et al. 2004

View full text Add to dashboard Cite

The objective of this study was to develop a pre-clinical large animal model for the in vivo hemodynamic testing of prosthetic valves in the aortic position without the need for cardiopulmonary bypass. Ten male pigs were used. A composite valved conduit was constructed in the operating room by implanting a prosthetic valve between two separate pieces of vascular conduits, which bypassed the ascending aorta to the descending aorta. Prior to applying a side-biting clamp to the ascending aorta for proximal grafting to the aortic anastomosis, an aorta to femoral artery shunt was placed just proximally to this clamp. The heart rate, cardiac output, Vmax, transvalvular pressure gradient, effective orifice area and incremental dobutamine stress response were assessed. A dose dependant increase with dobutamine was seen in terms of cardiac output, Vmax, and the peak transvalvular pressure gradient both in the native and in the prosthetic valve. However, the increment was much steeper in the prosthetic valve. No significant differences in cardiac output were noted between the native and the prosthetic valves. The described pre-clinical porcine model was found suitable for site-specific in-vivo hemodynamic assessment of aortic valvular prosthesis without cardiopulmonary bypass.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of an Animal Experimental Model for a Bileaflet Mechanical Heart Valve Prosthesis

et al. 2004

View full text Add to dashboard Cite

show abstract

“…As matter of fact, the choice of an appropriate animal for TEHV implantation largely determines the ability to achieve reproducible results, relevant to human application. Prior to the adoption of sheep, calves (15) and dogs (16) had been used with questionable results. The use of the ovine species has provided important data (17–20) and represents to date the standard model recommended by the Food and Drug Administration.…”

mentioning

confidence: 99%

Physiological Performance of a Detergent Decellularized Heart Valve Implanted for 15 Months in Vietnamese Pigs: Surgical Procedure, Follow‐up, and Explant Inspection

et al. 2012

View full text Add to dashboard Cite

This study features the longest experimental follow-up for decellularized heart valves implanted in an animal model. Porcine aortic heart valves were decellularized according to a disclosed standardized method in which TRITON X-100 and sodium cholate (TRICOL) are used in succession, followed by a further treatment with the endonuclease Benzonase to completely remove the nucleic acid remnants. Experimental animals (n = 17), represented by Vietnamese pigs (VPs), received a decellularized aortic allograft as a substitute for the replacement of their right ventricular outflow tract. The surgical implantation of the TRICOL-treated aortic valve conduit was successful in 11 VPs, while perioperative or postoperative complications occurred in the remaining six animals. In the sham-operated group (n = 4), the native pulmonary root was excised and immediately reimplanted orthotopically in the same animal. Echocardiography demonstrated a satisfactory hemodynamic performance of the TRICOL-treated valves during follow-up as well as the absence of relevant leaflet alterations concerning thickness and motility or valve insufficiency. At explantation, macroscopic inspection of tissue-engineered heart valve conduits did not evidence calcifications and showed a decreased wall thickness, comparable to that of the reimplanted native pulmonary roots. Noteworthy, extended functional performance, recovery of DNA content, and active extracellular matrix precursor incorporation are apparently compatible with the properties of a living self-supporting substitute.

show abstract