Background correction method for the determination of ascorbic acid in soft drinks, fruit juices and cordials using direct ultraviolet spectrophotometry

Lau, Oi-Wah; Luk, Shiu-Fai; Wong, Kit-Sum

doi:10.1039/an9861100665

Cited by 29 publications

(11 citation statements)

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“…Among these signals, crosstalk between plant hormones is believed to be one of the important aspects which control fruit development [4]- [7]. Specific to climacteric fruit such as apple, ethylene is well-known to play a pivotal role in the ripening regulation [8]- [11]. A previous study on the expression patterns of a pre-climacteric ethylene biosynthesis gene, i.e.…”

Section: Introductionmentioning

confidence: 99%

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (Malus × domestica) Fruit Maturation Patterns

Shin¹,

Lee²,

Rudell³

et al. 2015

AJPS

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Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely unknown. To understand the function of auxin during apple fruit maturation and ripening, auxin efflux carrier and IAA-amido synthetase encoding genes were identified from the apple genome based on the results of previous microarray analysis. The expression patterns of these genes were analyzed using qRT-PCR during 10 -12 weeks of fruit maturation for two apple cultivars: "Golden Delicious" (GD) and "Cripps Pink" (CP), which have the distinct patterns of maturation progression. Our results showed that the expressions of auxin efflux carrier and IAA-amido synthetase genes have a correlation with the timing of ethylene biosynthesis pathway activation in both cultivars. The earlier and stronger expression of MdGH3.102 and MdAECFP1 in the fruit of GD, a mid-season cultivar, correlates with the earlier activation of a pre-climacteric ethylene biosynthesis gene of MdACS3, compared with that in CP, a late-ripening apple cultivar. Results of exogenous IAA treatment indicated that the expression patterns of the genes were regulated in a fruit maturity dependent manner. Our results suggested that the dynamics of the auxin level in apple fruit cortex could be one of the key factors influencing the timing of ethylene biosynthesis pathway activation and consequently contributed to the control of the apple maturation progression.

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Section: Introductionmentioning

confidence: 99%

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (Malus × domestica) Fruit Maturation Patterns

Shin¹,

Lee²,

Rudell³

et al. 2015

AJPS

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show abstract

“…We compared our proposed method with a well-known background subtraction procedure reported by Lau and co-workers (20). We had used (25) this published procedure before and found that the method gave satisfactory results.…”

Section: Resultsmentioning

confidence: 85%

“…Pachla and co-workers (7) had reviewed the methods for analysis of both species. A great variety of techniques including colorimetry (8), gas (9) and high performance liquid chromatography (HPLC) (10,11), electrochemistry (12), microfluorimetry (13), and spectrophotometry (14)(15)(16)(17)(18)(19)(20) have been used to analyze ascorbic acid content in various kinds of samples such as foods, vitamins, and biological fluids. Deutsch and Weeks (13) developed what is now one of the standard methods for the determination of AA using fluorimetry.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Spectrophotometric Determination of Ascorbic Acid in Commercial Vitamin C Tablets

Esteban

1997

Microchemical Journal

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“…However, the rate of thermal decomposition is found to be very low 72 and fruit juice samples that are unstable to alkaline treatment, have fine particles, have a deep coloration or contain high concentrations of caffeine, saccharin, caramel and tannic acid can not be analyzed. Some methods based on the Cu(II)-catalyzed oxidation are reported for the assay of pharmaceuticals, fruits and beverages [74][75][76][77] allowing the determination of AA up to 120 µg ml -1 at λ max =267 nm. Fe(II) interferes seriously.…”

Section: Resultsmentioning

confidence: 99%

Photometric Methods for the Determination of Vitamin C

1998

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Background correction method for the determination of ascorbic acid in soft drinks, fruit juices and cordials using direct ultraviolet spectrophotometry

Cited by 29 publications

References 0 publications

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns

Enzymatic Spectrophotometric Determination of Ascorbic Acid in Commercial Vitamin C Tablets

Photometric Methods for the Determination of Vitamin C

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Background correction method for the determination of ascorbic acid in soft drinks, fruit juices and cordials using direct ultraviolet spectrophotometry

Cited by 29 publications

References 0 publications

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (&lt;i&gt;Malus&lt;/i&gt; &#215; &lt;i&gt;domestica&lt;/i&gt;) Fruit Maturation Patterns

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (&lt;i&gt;Malus&lt;/i&gt; &#215; &lt;i&gt;domestica&lt;/i&gt;) Fruit Maturation Patterns

Enzymatic Spectrophotometric Determination of Ascorbic Acid in Commercial Vitamin C Tablets

Photometric Methods for the Determination of Vitamin C

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Product

Resources

About

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns