Photometric Methods for the Determination of Vitamin C

Arya, Sunil; Mahajan, Meenakshi; Jain, Prashant K.

doi:10.2116/analsci.14.889

Cited by 92 publications

(55 citation statements)

References 75 publications

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“…Some of the techniques include: direct titration [15][16][17][18] fluorometric methods, chromatographic methods, and Electrochemical [6,[18][19][20][21][22]. However, some of these methods are time-consuming, some are costly, some need special training operators, or they suffer from the insufficient sensitivity or selectivity [6].…”

Section: Introductionmentioning

confidence: 99%

Iodometric Determination of the Ascorbic Acid (Vitamin C) content of mango and tomato consumed in Mettu Town Ilu Abba Bora Zone, Oromia Ethiopia.

Motora¹

2017

IOSRJPBS

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Section: Introductionmentioning

confidence: 99%

Iodometric Determination of the Ascorbic Acid (Vitamin C) content of mango and tomato consumed in Mettu Town Ilu Abba Bora Zone, Oromia Ethiopia.

Motora¹

2017

IOSRJPBS

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“…This primarily is because researchers believe that the coupling of chromatographic separation with spectrophotometric, fluorometric, or electrochemical detection makes HPLC analysis far more specific than traditional redox methods. Reports that HPLC permits simultaneous determination of most watersoluble vitamins and their different forms have been documented (Arya et al, 2000). Uncertainties still remain regarding the agreed HPLC method for the determination of vitamin B 12 since researchers say HPLC is unsuitable for vitamin B12 analysis in food because of their low concentration typically found (Ellenbogen and Cooper, 1992).…”

Section: Simultaneous Determination Of Water-soluble Vitaminsmentioning

confidence: 99%

“…In addition, the use of an acidic mobile phase might affect the columns life of columns packed with silica as silicate is dissolved slowly by such solutions. Though acetonitrile has most commonly been used as a mobile phase, handling of this solvent requires precaution to avoid breathing its vapour (Arya et al, 2000).…”

Section: Chromatographic Conditions For Determination Of Water-solublmentioning

confidence: 99%

“…Today, the global market is flooded with water-soluble vitamins fortified foods and nutritional supplements. Keeping in mind their importance, the analysis of food products and pharmaceuticals containing these vitamins assume significance in their accurate assays (Arya et al, 2000). Even though the literature is filled up with different simultaneous analysis of the water-soluble vitamins, the high performance liquid chromatography (HPLC) has assumed wide applications because of its sensitivity, selectivity, and utility-based usability.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

Abano

Dadzie

2014

Croat. J. Food Sci. Technol.

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SummaryThe water-soluble vitamins (WSV): ascorbic acid (vitamin C), thiamine (B1), riboflavin (B2), niacin (B3), panthothenic acid (B5), pyridoxine, and pyridoxal (B6), folic acid (B9), biotin(B8) , and B12 are very essential in the diet of humankind. As a result of ever increasing pressures from both consumers and legal enforcers, to specify accurately nutritive compositions of WSV that are present in food materials, many researchers have attempted to fill this niche through the provision of highly sensitive and rapid high performance liquid chromatography (HPLC) procedures. In view of the health benefits of WSV, a replete of HPLC methods have been developed for simultaneous determination of their contents in nature and fortified food samples, nutritional supplements, as well as blood plasmas. The rate of losses of these vitamins during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive HPLC procedure for their simultaneous separations and assays. This review critically assesses the different HPLC procedures developed by researchers and available in the open literature for simultaneous determination of water-soluble vitamins (WSV) in dried tropical fruits materials. The study revealed that not a single chromatographic run developed by researchers can simultaneously elute all the WSV at a time. However, the HPLC procedures that are capable of determining all the WSV were coupled with electrospray ionization mass spectroscopy (ESI-MS), thus making the set-up expensive.

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“…The determination of ascorbic acid (AA) has gained increased significance in pharmaceutic, clinical and food applications. Numerous papers and reviews [1][2][3] have described the determination of AA. The methods used include titrimetric, spectrophotometric, fluorometric, electrochemical, chromatographic, kinetic and chemiluminescence procedures.…”

Section: Introductionmentioning

confidence: 99%

A New Spectrofluorometric Method for the Determination of Ascorbic Acid Based on Its Activating Effect on a Hemoglobin-Catalyzed Reaction

et al. 2002

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Ascorbic acid is an important vitamin which participates in a great variety of biological events concerning electron-transport reactions, hydroxylations, the oxidative catabolism of aromatic amino acids and so on. The determination of ascorbic acid (AA) has gained increased significance in pharmaceutic, clinical and food applications. Numerous papers and reviews 1-3 have described the determination of AA. The methods used include titrimetric, spectrophotometric, fluorometric, electrochemical, chromatographic, kinetic and chemiluminescence procedures.Enzymatic assays have considerable advantages over the traditional analytical procedures because of their rapidity and high selectivity. 4 The growing interest in enzymatic methods for the determination of AA has been demonstrated in reports in which ascorbic acid oxidase (EC 1.11.1.17) or peroxidase (EC 1.10.3.3) was used. In the ascorbic acid oxidase-based determination, 5 AA was oxidized with oxygen catalyzed by the enzyme.In the peroxidase-catalyzed method, 6 the determination was based on the interference of AA with the peroxidase-catalyzed reaction. 7 Besides, Huang et al. 8 carried out a laccase (EC 1.10.3.2)-based micellar enhanced spectrofluorometric determination of ascorbic acid.However, many enzymes are expensive and unstable. Chemists and biologists have often attempted to mimic the biological properties of such natural systems. As we know, the heme iron acts as an activity center of horseradish peroxidase (HRP), which was used by Zhu et al. 9 to determine AA. It was based on the interference of AA on the peroxidase activity. Other workers 10 introduced some synthesized metalloporphyin to act as substitutes for HRP, which were also applied to an enzymatic assay for AA. However, simple natural or synthetic metalloporphyrins do not show satisfactory activity and selectivity because they lack the spatial structure of a natural enzyme, which is essential for the special inclusion behavior between the enzyme and the substrate. Hemoglobin (Hb), a necessary vehicle for oxygen carriage in the body, has the natural quaternary structure as enzymes. It contains four subunits of polypeptide, and each polypeptide chain contains a heme group that may be able to serve as the active center. Encouraged by reports on studies of peroxidase substitutes and the natural spatial structure of Hb, as well as its cheaper price, we expected Hb to be a mimic enzyme for HRP. Up to the present, there has been no report on the application of Hb to the analysis AA.In this work, based on the Hb-catalyzed reaction, we found that a small amount of AA could strongly enhance both the fluorescence intensity and reaction rate. What is more, there is a good linearity between the fluorescent intensity change and the amount of AA at low concentration. Thus, a highly sensitive spectrofluorometry for AA was established. Experimental ReagentsUnless stated otherwise, deionized and distilled water was used throughout. p-Cresol (Aldrich) was 99% pure; a stock solution was prepared by mixing 0.15 mL o...

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Photometric Methods for the Determination of Vitamin C

Cited by 92 publications

References 75 publications

Iodometric Determination of the Ascorbic Acid (Vitamin C) content of mango and tomato consumed in Mettu Town Ilu Abba Bora Zone, Oromia Ethiopia.

Iodometric Determination of the Ascorbic Acid (Vitamin C) content of mango and tomato consumed in Mettu Town Ilu Abba Bora Zone, Oromia Ethiopia.

Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

A New Spectrofluorometric Method for the Determination of Ascorbic Acid Based on Its Activating Effect on a Hemoglobin-Catalyzed Reaction

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