Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain

Ruiz-García, Cristina; Béjar, Victoria; Martínez‐Checa, Fernando; Llamas, Inmaculada; Quesada, Emilia

doi:10.1099/ijs.0.63310-0

Cited by 233 publications

(161 citation statements)

References 21 publications

(19 reference statements)

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“…The cell-wall peptidoglycan contained meso-diaminopimelic acid. The total cellular fatty acid profiles showed a large amount of branched fatty acids; the major components (>5.0 %) were anteiso-C 15 : 0 (34.8 %), iso-C 15 : 0 (21.9 %), iso-C 17 : 0 (15.7 %), anteiso-C 17 : 0 (11.4 %) and C 16 : 0 (9.7 %) ( the close relatives B. amyloliquefaciens, B. atrophaeus and B. velezensis determined in this study were consistent with previously reported profiles (Borriss et al, 2011;Nakamura, 1989;Ruiz-García et al, 2005). B. nakamurai could not be distinguished from its closest related species on the basis of fatty acid profiles.…”

supporting

confidence: 82%

Bacillus nakamurai sp. nov., a black-pigment-producing strain

Dunlap

Saunders

Schisler

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Two isolates of a Gram-stain-positive, strictly aerobic, motile, rod-shaped, endospore-forming bacterium were identified during a survey of the Bacillus diversity of the Agriculture Research Service Culture Collection. These strains were originally isolated from soil and have a phenotype of producing a dark pigment on tryptic soy agar. Phylogenetic analysis of the 16S rRNA gene indicated that these strains were related most closely to Bacillus subtilis subsp. inaquosorum (99.7 % similarity) and Bacillus axarquiensis (99.7 %). In phenotypic characterization, the novel strains were found to grow between 17 and 50 C and can tolerate up to 9 % (w/v) NaCl. Furthermore, the strains grew in media of pH 5.5-10 (optimal growth at pH 7.0-8.0). The predominant cellular fatty acids were anteiso-C 15 : 0 (34.8 %) and iso-C 15 : 0 (21.9 %). The cell-wall peptidoglycan contained meso-diaminopimelic acid. A draft genome of both strains was completed. The DNA G+C content was 43.8 mol%. A phylogenomic analysis on the core genome of these two new strains and all members of the Bacillus subtilis group revealed these two strains formed a distinct monophyletic clade with the nearest neighbour Bacillus amyloliquefaciens. DNA-DNA relatedness studies using in silico DNA-DNA hybridizations showed the two strains were conspecific (93.8 %), while values with all other species (<31.5 %) were well below the species threshold of 70 %. Based on the consensus of phylogenetic and phenotypic analyses, these strains are considered to represent a novel species within the genus Bacillus, for which the name Bacillus nakamurai sp. nov. is proposed, with type strain NRRL B-41091 T (=CCUG 68786 T ).

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supporting

confidence: 82%

Bacillus nakamurai sp. nov., a black-pigment-producing strain

Dunlap

Saunders

Schisler

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…DNA was extracted and purified from whole cells by the phenol method (Saito and Miura, 1963). 16S rRNA gene sequencing was carried out (Shida et al, 1996) by using the 27F and 1488R rRNA primers (Ruiz-Garcia, 2005). The five housekeeping genes were amplified using a set of primer as described by Juneja and Lazzaro (2009).…”

Section: Methodsmentioning

confidence: 99%

Providencia thailandensis sp. nov., isolated from seafood processing wastewater

Khunthongpan

Sumpavapol

Tanasupawat

2013

J. Gen. Appl. Microbiol.

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“…nov. was isolated during a research program to discover novel bacterial strains capable of synthesizing new lipopeptides with surfactant and/or antimicrobial activity. Genomic and phenotypic data demonstrated that B. velezensis is a novel species of Bacillus (Ruiz-García et al, 2005), now reclassified as B. amyloliquefaciens (Wang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of <i>Bacillus velezensis</i> and <i>Glomus intraradices</i> on Fruit Quality and Growth Parameters in Strawberry Soilless Growing System

et al. 2015

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This study evaluates the effect on the 'Splendor' and 'Primoris' strawberry cultivars of different dates of inoculation with Glomus intraradices, an arbuscular mycorrhizal (AM) fungus. Additionally, plants were grown in a soilless growing system with or without Bacillus velezensis at the beginning of the experiment. A completely randomized block design (2 biofertilizer treatments × 2 cultivars × 3 inoculation dates) with 2 replications was used. Each replicate consisted of one bag with 10 plants. Fruit weight, fruit quality, growth parameters, and SPAD values in young leaves were monitored from October 2011 to June 2012 in a greenhouse. At the end of the crop cycle, the microbial population of Bacillus spp. and the Glomus intraradices population were determined from the rhizosphere of the plant. Bacillus velezensis and Glomus intraradices were established in the strawberry soilless growing system. The effect of arbuscular mycorrhizae on strawberry fruit quality was more important than that on growth parameters. Biofertilizer with arbuscular mycorrhizal fungi had an inhibitory effect on fruit quality, as indicated by low TSS, pH, and TA values. The combined effect of the biofertilizer and inoculation dates of Glomus intraradices on growth parameters was more significant in the 'Primoris' cultivar than in 'Splendor'. In both cultivars, an increase in SPAD values was observed from week 12 to week 22 after planting. Depending on the cultivar selected, the date of inoculation may significantly affect plant response to AM fungal colonization in a soilless growing system.

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Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain

Cited by 233 publications

References 21 publications

Bacillus nakamurai sp. nov., a black-pigment-producing strain

Bacillus nakamurai sp. nov., a black-pigment-producing strain

Providencia thailandensis sp. nov., isolated from seafood processing wastewater

Effect of <i>Bacillus velezensis</i> and <i>Glomus intraradices</i> on Fruit Quality and Growth Parameters in Strawberry Soilless Growing System

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