Bacillus velezensis GF267 as a multi-site antagonist for the control of tomato bacterial spot

Mates, Aveline de Paula Kuyat; Pontes, Nadson de Carvalho; Halfeld-Vieira, B. de A.

doi:10.1016/j.biocontrol.2019.104013

Cited by 16 publications

(6 citation statements)

References 35 publications

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“…Similar to most biocontrol Bacillus spp., the biocontrol effects of B. velezensis include competition with pathogenic microorganisms for nutrition and space, promotion of inducible systemic resistance in plants, and secretion of antimicrobial ingredients. 28,33 The antifungal substances produced by B. velezensis include volatile organic compounds such as ketones, alcohols, and alkanes, 34 as well as antifungal proteins, antifungal peptides, and aminoglycosides. 33,35,36 Among them, lipopeptides form an important class of antifungal components whose biosynthesis is catalyzed by nonribosomal polypeptide synthase to complete their biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Study on the Biocontrol Potential of Antifungal Peptides Produced by Bacillus velezensis against Fusarium solani That Infects the Passion Fruit Passiflora edulis

Wang

et al. 2021

J. Agric. Food Chem.

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A bacterium identified as Bacillus velezensis with a growth inhibitory effect against Fusarium solani, a pathogen that caused basal stem rot in the passion fruit Passiflora edulis, was isolated in this study. From the fermentation broth of B. velezensis, a type of antifungal peptide (named BVAP) with a molecular weight of ca. 1.5 kDa was purified and found to be fengycin. BVAP suppressed mycelial growth in F. solani with an IC50 of 5.58 μg/mL, which was superior to those of the chemical fungicides thiram (41.24 μg/mL) and hymexazol (343.31 μg/mL). The antifungal activity remained stable after exposure to 50–100 °C or following incubation with solutions at pH 1–3. Further research revealed that BVAP increased the permeability of the F. solani mycelial membrane, brought about swelling at the tips of hyphae, and elicited abnormal accumulation of nucleic acids and chitin at the sites of swelling. These findings indicate that BVAP possessed a remarkable biocontrol potential toward F. solani.

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Section: Discussionmentioning

confidence: 99%

Study on the Biocontrol Potential of Antifungal Peptides Produced by Bacillus velezensis against Fusarium solani That Infects the Passion Fruit Passiflora edulis

Wang

et al. 2021

J. Agric. Food Chem.

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“…For instance, foliar application of tailocins (phage-tail-like bacteriocins produced by Pseudomonas fluorescens SF4c) against X. vesicatoria and Bacillus velezensis GF267 against X. euvesicatoria pv. perforans reduces the disease severity and incidence in the greenhouse (de Paula Kuyat Mates et al, 2019;Príncipe et al, 2018). The plant growth-promoting rhizobacterium (PGPR) Bacillus pumilus S2-3-2 and a mixture of Bacillus spp.…”

Section: Biological Controlmentioning

confidence: 99%

“…euvesicatoria pv. perforans reduces the disease severity and incidence in the greenhouse (de Paula Kuyat Mates et al, 2019 ; Príncipe et al, 2018 ). The plant growth‐promoting rhizobacterium (PGPR) Bacillus pumilus S2‐3‐2 and a mixture of Bacillus spp.…”

Section: Managementmentioning

confidence: 99%

A centenary for bacterial spot of tomato and pepper

Osdaghi

Jones

Sharma

et al. 2021

Molecular Plant Pathology

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“…Bacillus velezensis is a novel species in the Bacillus genus that exhibits a broad spectrum of antimicrobial activities because it produces various secondary metabolites (Pereira et al 2019). Indeed, it is widely used in the biological control of animal and plant diseases (de Paula Kuyat Mates et al 2019;Rabbee et al 2019;Thurlow et al 2019;Wang et al 2019b), and has been shown to antagonize a variety of bacterial pathogens isolated from aquatic animals, representing a current hotspot in the biological control of aquatic bacterial diseases (Yi et al 2018;Wang et al 2019b;Zhang et al 2019b).…”

Section: Introductionmentioning

confidence: 99%

Bacillus velezensis WLYS23 strain possesses antagonistic activity against hybrid snakehead bacterial pathogens

et al. 2021

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Aim:The aims of this study were to screen an antagonistic probiotic for the prevention and control of bacterial diseases in snakehead fish and to evaluate the antimicrobial activities, biosafety and biocontrol effect of the antagonistic strain. Methods and Results: In this study, the WLYS23 strain exhibiting the most effective antagonistic properties against several fish pathogens was selected from the intestine of healthy snakehead fish. The strain was identified as Bacillus velezensis based on morphological, physiological and biochemical characteristics, and phylogenetic analysis. This strain showed amylase, cellulase, protease and lipase activities according to extracellular enzyme activity assays. Moreover, the cell-free extract of the WLYS23 strain exhibited strong antibacterial activity, with MICs of ≤64 lg ml À1 for most fish pathogens. Additionally, the cell-free extract was heat and pH stable, and resistant to protease, whereas their antimicrobial activities were significantly influenced by metal ions at high concentration. The WLYS23 strain and its cell-free extract were safe for snakehead and zebrafish. The disease resistance of snakehead fish was significantly increased following administration of the WLYS23 strain and its cell-free extract respectively. The complete genome of the WLYS23 strain was sequenced and found to harbour seven gene clusters encoding secondary metabolites with antimicrobial activity. Conclusion:The WLYS23 strain possesses great potential as a biocontrol agent, which can be commercially developed to improve disease control in freshwater aquaculture. Significance and Impact of the Study: Snakehead fish are important commercially farmed fish worldwide. However, the bacterial diseases caused by Aeromonas sp. and Nocardia seriolae in farmed snakehead fish lead to huge economic losses. Safe, economical and efficient probiotics are limited to prevent and control these diseases. Here, we provide a promising biocontrol agent with antagonistic activity against bacterial diseases of snakehead.

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Bacillus velezensis GF267 as a multi-site antagonist for the control of tomato bacterial spot

Cited by 16 publications

References 35 publications

Study on the Biocontrol Potential of Antifungal Peptides Produced by Bacillus velezensis against Fusarium solani That Infects the Passion Fruit Passiflora edulis

Study on the Biocontrol Potential of Antifungal Peptides Produced by Bacillus velezensis against Fusarium solani That Infects the Passion Fruit Passiflora edulis

A centenary for bacterial spot of tomato and pepper

Bacillus velezensis WLYS23 strain possesses antagonistic activity against hybrid snakehead bacterial pathogens

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