Bacillus thuringiensis Recombinant Insecticidal Protein Production

Schnepf, H E

doi:10.1007/978-94-007-3021-2_14

Cited by 3 publications

(2 citation statements)

References 76 publications

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“…The entomopathogenic gram‐positive soil bacterium Bacillus thuringiensis is extensively used as a bio‐pesticide across the world (Schnepf et al. 1998).…”

Section: Introductionmentioning

confidence: 99%

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Identification of native Bacillus thuringiensis strain from South India having specific cytocidal activity against cancer cells

Kkani¹,

Selvanayagam²,

Shenbagarathai³

2010

Journal of Applied Microbiology

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Aim: To identify the parasporin‐producing, indigenous Bacillus thuringiensis strains that specifically targets human cancer cells in Madurai, Tamil Nadu, South India. Methods and Results: Alkali‐solubilized inclusion proteins from the 82 nonclonal indigenous isolates of B. thuringiensis were analysed for their cytotoxicity against two human cancer cell lines, U‐937 (human histiocytic lymphoma) and HCT‐250 (adherent human colon cancer cells). Activated inclusion protein from one of the isolates, B. thuringiensis LDC‐391, was found to be highly cytotoxic to HCT‐250 and moderately toxic to U‐937, but nontoxic to normal lymphocytes. This strain did not show any insecticidal activity against the lepidopteran and dipteran larvae tested, as well as it was nonhaemolytic on human erythrocytes. The Western‐blotting analysis showed that the putative 180 kDa cytotoxic protein from the isolate B. thuringiensis LDC‐391 cross‐reacted with the reference antisera of 81‐kDa parasporin‐1. Conclusions: Our observations imply that B. thuringiensis LDC‐391 is different from the already reported parasporin producers, as it is showing variation in the target specificity. Significance and Impact of the Study: Characterizing these proteins can pave the way to alleviate problems associated with neoplastic transformation and cancer progression.

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“…The entomopathogenic gram‐positive soil bacterium Bacillus thuringiensis is extensively used as a bio‐pesticide across the world (Schnepf et al. 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies were conducted to identify other biological functions of the parasporal inclusion proteins of noninsecticidal strains. Some of the B. thuringiensis strains were reported to show toxicity against mites, platyhelminthes, nematodes and pathogenic protozoans such as Plasmodium berghei (Schnepf et al. 1998; Zhaohui et al.…”

Section: Introductionmentioning

confidence: 99%

Identification of native Bacillus thuringiensis strain from South India having specific cytocidal activity against cancer cells

Kkani¹,

Selvanayagam²,

Shenbagarathai³

2010

Journal of Applied Microbiology

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Multifaceted interactions between the pseudomonads and insects: mechanisms and prospects

2021

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Early transcription of Bacillus thuringiensis cry genes in strains active on Lepidopteran species and the role of gene content on their expression

Porcar

Déleclusse

Ibarra

et al. 2014

Antonie van Leeuwenhoek

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Six strains of Bacillus thuringiensis previously selected as highly toxic against Manduca sexta and Plutella xylostella were analyzed by PCR screening in order to identify the cry genes active on Lepidoptera. According to their gene content and insecticidal potency, these strains were cultured and aliquots taken at different pre- and post-sporulation times. Total RNA was extracted and used as template in RT-PCR analyses directed to identify mRNAs of the previously identified cry genes. Results showed transcription of genes cry1A, cry1E, cry1I, and cry2 even before the onset of sporulation. However, this early transcription did not lead to an appreciable parasporal protein synthesis until t5-t9, as deduced from SDS-PAGE profiles. As for cry1I gene, the corresponding protein was not detected, as expected, but cry1I mRNAs were present at least until t5. Interestingly, strains expressing four cry genes from the end of the log phase onwards exhibited kinetics characterized by a very long transition phase, whereas the strain expressing only one cry gene showed a very short transition phase. Strains expressing three genes showed an intermediate profile. These results indicate that the transcription of B. thuringiensis cry1 and cry2 genes in natural strains can start several hours before massive crystal synthesis occurs and that this translation is probably competing with transcriptional regulators required for the sporulation onset.

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Bacillus thuringiensis Recombinant Insecticidal Protein Production

Cited by 3 publications

References 76 publications

Identification of native Bacillus thuringiensis strain from South India having specific cytocidal activity against cancer cells

Identification of native Bacillus thuringiensis strain from South India having specific cytocidal activity against cancer cells

Multifaceted interactions between the pseudomonads and insects: mechanisms and prospects

Early transcription of Bacillus thuringiensis cry genes in strains active on Lepidopteran species and the role of gene content on their expression

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