Paederus beetles are notorious for their irritant haemolymph toxin pederin synthesized by an unculturable bacterial symbiont which causes dermatitis when in contact with human skin. Pederin polymorphism is observed when (+) females carrying the toxin pederin and (−) females lacking this, co-occur in natural populations. Despite reports detecting pederin polymorphism in several Paederina beetles, symbiont infection frequencies in their natural populations and the bacterial diversity differences underlying the polymorphism between these female types are understudied. Herein we report a high prevalence (>80%) of female Paederus fuscipes carrying bacterial ped genes in all six study populations. This finding suggests selection pressure favouring pederin-producing females is crucial for survival in the natural environment. 16S rRNA metabarcoding analysis revealed significant dominance of the unculturable pederin-producing Pseudomonas-like bacterium (Gammaproteobacteria) in (+) females consistent with previous studies. The microbial diversity of the (−) females revealed significant abundance of Apibacter (Bacteroidia) previously undocumented suggesting its importance in the functionality of (−) females. Predicted functions related to metabolisms are enriched in (−) females suggesting fitness advantage possibilities in sustaining the population in the absence of predation. Further investigations on possible genetic basis of host genotype revealed no association of host mtDNA and pederin polymorphism in P. fuscipes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.