Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots

Cao, Yun; Zhen-hua, Zhang; Ling, Ning; Yuan, Yujuan; Zheng, Xinyan; Shen, Biao; Shen, Qirong

doi:10.1007/s00374-011-0556-2

Cited by 301 publications

(221 citation statements)

References 47 publications

Supporting

Mentioning

195

Contrasting

Unclassified

Order By: Relevance

“…subtilis SQR9 (now reidentified as B. amyloliquefaciens) isolated from cucumber rhizosphere suppressed the growth of F. oxysporum in the rhizosphere and protected the host from pathogen invasion through efficient root colonization (26). In vitro testing showed that the supernatant of B. amyloliquefaciens SQR9 completely inhibited the conidial germination of F. oxysporum, implying that it might contain antifungal compounds.…”

mentioning

confidence: 99%

Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

Shao

et al. 2013

Appl Environ Microbiol

Self Cite

197

138

View full text Add to dashboard Cite

cBacillus amyloliquefaciens strains are capable of suppressing soilborne pathogens through the secretion of an array of lipopeptides and root colonization, and biofilm formation ability is considered a prerequisite for efficient root colonization. In this study, we report that one of the lipopeptide compounds (bacillomycin D) produced by the rhizosphere strain Bacillus amyloliquefaciens SQR9 not only plays a vital role in the antagonistic activity against Fusarium oxysporum but also affects the expression of the genes involved in biofilm formation. When the bacillomycin D and fengycin synthesis pathways were individually disrupted, mutant SQR9M1, which was deficient in the production of bacillomycin D, only showed minor antagonistic activity against F. oxysporum, but another mutant, SQR9M2, which was deficient in production of fengycin, showed antagonistic activity equivalent to that of the wild-type strain of B. amyloliquefaciens SQR9. The results from in vitro, root in situ, and quantitative reverse transcription-PCR studies demonstrated that bacillomycin D contributes to the establishment of biofilms. Interestingly, the addition of bacillomycin D could significantly increase the expression levels of kinC gene, but KinC activation is not triggered by leaking of potassium. These findings suggest that bacillomycin D contributes not only to biocontrol activity but also to biofilm formation in strain B. amyloliquefaciens SQR9.

show abstract

mentioning

confidence: 99%

Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

Shao

et al. 2013

Appl Environ Microbiol

Self Cite

197

138

View full text Add to dashboard Cite

show abstract

“…amyloliquefaciens SQR9, which was isolated from the cucumber (Cucumis sativus Linn.) rhizosphere, suppressed the growth of Fusarium oxysporum, the causative agent of vascular wilt of cucumber (12). SQR9 produces three families of lipopeptides: bacillomycin D, fengycins, and surfactins.…”

mentioning

confidence: 99%

Enhanced Control of Cucumber Wilt Disease by Bacillus amyloliquefaciens SQR9 by Altering the Regulation of Its DegU Phosphorylation

Zhang

Wang

et al. 2014

Appl Environ Microbiol

Self Cite

107

View full text Add to dashboard Cite

Bacillus amyloliquefaciens strain SQR9, isolated from the cucumber rhizosphere, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion through efficient root colonization. In the Gram-positive bacterium Bacillus, the response regulator DegU regulates genetic competence, swarming motility, biofilm formation, complex colony architecture, and protease production. In this study, we report that stepwise phosphorylation of DegU in B. amyloliquefaciens SQR9 can influence biocontrol activity by coordinating multicellular behavior and regulating the synthesis of antibiotics. Results from in vitro and in situ experiments and quantitative PCR (qPCR) studies demonstrate the following: (i) that the lowest level of phosphorylated DegU (DegUϳP) (the degQ mutation) impairs complex colony architecture, biofilm formation, colonization activities, and biocontrol efficiency of Fusarium wilt disease but increases the production of macrolactin and bacillaene, and (ii) that increasing the level of DegUϳP by degQ and degSU overexpression significantly improves complex colony architecture, biofilm formation, colonization activities, production of the antibiotics bacillomycin D and difficidin, and efficiency of biocontrol of Fusarium wilt disease. The results offer a new strategy to enhance the biocontrol efficacy of Bacillus amyloliquefaciens SQR9.

show abstract

“…We speculate that F-6-gfp can form a biofilm, creating a hostile environment for the growth of pathogenic Fusarium. Cao et al [32] reported that B. subtilis SQR 9 could promote plant growth and control Fusarium wilt in cucumber by colonizing the plant roots. Ren et al [27] have suggested that the colonization of tobacco roots by Paenibacillus polymyxa C5 provides a mechanism to support the protection of tobacco plants from infection by pathogens.…”

Section: Discussionmentioning

confidence: 99%

Effects of <i>Bacillus cereus</i> F-6 on Promoting Vanilla (<i>Vanilla planifolia</i> Andrews.) Plant Growth and Controlling Stem and Root Rot Disease

Zhao¹,

Wang²,

Zhu³

et al. 2015

View full text Add to dashboard Cite

A lipopeptide-producing bacterium, Bacillus cereus (F-6), was isolated from the rhizosphere soil of a healthy vanilla (Vanilla planifolia) plant cultivated on a plantation under 21 years of continuous cropping with vanilla. A pot experiment was conducted to investigate the effects of the green fluorescent protein-tagged F-6 (F-6-gfp) and its bio-organic fertilizer (BIO) on vanilla plant growth and stem and root rot disease, using the same plantation soil. The application of BIO significantly increased the vanilla plant root, stem and leave dry weights; however, there was not a significant difference between the F-6-gfp-inoculated treatment and the control. Meanwhile, the BIO application also significantly reduced the severity of stem and root rot disease compared to the control. The rhizosphere soil population of Fusarium was approximately 10-fold smaller in the BIO treatment compared to the control treatment at 150 days after transplantation. The number of B. cereus F-6-gfp in the rhizosphere soil of the BIO treatment remained significantly higher than that of the F-6-gfp-inoculated treatment throughout the experiment. In conclusion, F-6-gfp successfully colonized the rhizosphere soil in the BIO treatment, promoting vanilla plant growth, reducing the disease severity index, and decreasing the Fusarium population number, helping to remove barriers to the continuous cropping of vanilla.

show abstract

Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots

Cited by 301 publications

References 47 publications

Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

Enhanced Control of Cucumber Wilt Disease by Bacillus amyloliquefaciens SQR9 by Altering the Regulation of Its DegU Phosphorylation

Effects of <i>Bacillus cereus</i> F-6 on Promoting Vanilla (<i>Vanilla planifolia</i> Andrews.) Plant Growth and Controlling Stem and Root Rot Disease

Contact Info

Product

Resources

About

Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots

Cited by 301 publications

References 47 publications

Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

Enhanced Control of Cucumber Wilt Disease by Bacillus amyloliquefaciens SQR9 by Altering the Regulation of Its DegU Phosphorylation

Effects of &lt;i&gt;Bacillus cereus&lt;/i&gt; F-6 on Promoting Vanilla (&lt;i&gt;Vanilla planifolia&lt;/i&gt; Andrews.) Plant Growth and Controlling Stem and Root Rot Disease

Contact Info

Product

Resources

About

Effects of <i>Bacillus cereus</i> F-6 on Promoting Vanilla (<i>Vanilla planifolia</i> Andrews.) Plant Growth and Controlling Stem and Root Rot Disease