Bacillus subtilis expressing double-strand RNAs (dsRNAs) induces RNA interference mechanism (RNAi) and increases survival of WSSV-challenged Litopenaeus vannamei

Riet, Jade; Costa-Filho, João; Dall’Agno, Laura; Medeiros, Luiza; Azevedo, Raíza; Nogueira, Luiz Fagner Ferreira; Maggioni, Rodrigo; Pedrosa, Virgínia Fonseca; Romano, Luís Alberto; Altenbuchner, Josef; Wasielesky, Wilson; Marins, Luís Fernando

doi:10.1016/j.aquaculture.2021.736834

Cited by 10 publications

(3 citation statements)

References 64 publications

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“…Several probiotics have been suggested to improve the survival rate of L. vannamei shrimp against WSSV in vivo (Chai et al, 2016; Peraza‐Gómez et al, 2009; Riet et al, 2021). In the present study, the survival rate of shrimps fed on P. pentosaceus AB01 was significantly higher than that of the control group upon the WSSV infection.…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with Pediococcus pentosaceus enhances the innate immune response in and promotes growth of Litopenaeus vannamei shrimp

Kim

Phước

et al. 2022

Journal of Fish Diseases

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To reach the sustainable development goals on health management in Litopenaeus vannamei shrimp culture, Pediococcus pentosaceus AB01 was supplemented in shrimp diet. In this study, the control diet and three experimental diets containing P. pentosaceus AB01 (108, 109, 1010 CFU/g) were separately introduced to L. vannamei for a 28 days feeding trial. After the feeding trial, percent weight gain, feeding efficiency, and feed conversion ratio (FCR) were significantly elevated in L. vannamei administered with P. pentosaceus AB01 at 109 and 1010 colony‐forming unit (CFU)/g. Protease, amylase, and trypsin were found at higher levels in the probiotic‐supplied groups. The feeding of shrimps with P. pentosaceus AB01 significantly increased innate immune response and levels of related biochemical parameters in the haemolymph. After the white spot syndrome virus (WSSV) challenge, supplementation of P. pentosaceus AB01 had significant positive effects (p < .05) on survival rate, compared to that of the control diet. The higher resistance of L. vannamei to WSSV might have been due to alterations in the gut microbiome composition and upregulation of the Toll‐Like Receptor (TLR) signalling pathway. Hence, P. pentosaceus AB01 may be a promising alternative feed to promote growth rate, modulate microbiota composition, and enhance immunity in L. vannamei shrimp.

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Section: Discussionmentioning

confidence: 99%

Dietary supplementation with Pediococcus pentosaceus enhances the innate immune response in and promotes growth of Litopenaeus vannamei shrimp

Kim

Phước

et al. 2022

Journal of Fish Diseases

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“…After centrifugation, the supernatant was discarded and 2 mL of saline solution (0.9%) NaCl was added to wash the pellet, and this process was repeated twice (adapted from Zokaeifar et al 2012). Then, the pellet was diluted in saline solution so that each gram of feed received 10 10 colony-forming units (CFU) of the probiotic strains (Riet et al 2021). The commercial feed used (Guabi, Brazil) is free of probiotics and contains 35% crude protein, 9% lipids, 15% ash and 1.5% total phosphorous.…”

Section: Preparation Of Feed Supplemented With Probiotic Bacteriamentioning

confidence: 99%

“…Inoculums of the probiotic strains were rst cultivated for 16 h at 37 ºC in volumes of 3 mL in minimal salts medium (Anagnostopoulos and Spizizen, 1960), in a shaker incubator at 250 rpm. The next day, the cultures were added to erlenmayers (125 ml) containing 50 ml of the culture medium suggested by Riet et al (2021), which consists of a minimal salts medium with glucose and casein hydrolyzate. The cultures followed the same protocol used for culture of inocula, but the concentration of bacteria was monitored in a spectrophotometer until reaching the stationary phase (OD 600 = 1.6).…”

Section: Preparation Of Feed Supplemented With Probiotic Bacteriamentioning

confidence: 99%

Recombinant Bacillus subtilis expressing a fungal phytase as a probiotic additive in the diet of Pacific white shrimp Litopenaeus vannamei

Medeiros

Nornberg

Azevedo

et al. 2022

Preprint

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Phosphorus is an essential mineral present in the vegetable matter in the form of phytate, which is considered an antinutritional factor. Phytate can be degraded by phytases, which have been used in commercial feeds. However, these enzymes undergo costly isolation and purification processes. In the present study, a genetically modified (GM) Bacillus subtilis strain that expresses a fungal phytase was used as a feed additive. The GM probiotic was added to the commercial feed of shrimp Litopenaeus vannamei and its effects on zootechnical performance, proximate composition of muscle, lipid concentration in hepatopancreas and expression of genes related to digestion, amino acid metabolism and antioxidant defenses were analyzed. Although the genetically modified probiotic had no impact on growth parameters, there was a 39% increase in phosphorus content in muscle. In addition, genes related to digestion were downregulated in shrimp hepatopancreas, as well as an increase in lipids in this tissue. These results demonstrates that the GM probiotic increased the efficiency of the use of plant-derived phosphorus, which may imply a decrease in the addition of this element in the diets, as well as minimizing the impact of shrimp farms on the eutrophication of adjacent ecosystems.

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Shellfish Microbiome and Probiotics: A Decade in Review

Tarnecki,

Burgos

2023

Microbiome of Finfish and Shellfish

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Bacillus subtilis expressing double-strand RNAs (dsRNAs) induces RNA interference mechanism (RNAi) and increases survival of WSSV-challenged Litopenaeus vannamei

Cited by 10 publications

References 64 publications

Dietary supplementation with Pediococcus pentosaceus enhances the innate immune response in and promotes growth of Litopenaeus vannamei shrimp

Dietary supplementation with Pediococcus pentosaceus enhances the innate immune response in and promotes growth of Litopenaeus vannamei shrimp

Recombinant Bacillus subtilis expressing a fungal phytase as a probiotic additive in the diet of Pacific white shrimp Litopenaeus vannamei

Shellfish Microbiome and Probiotics: A Decade in Review

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