Dietary supplementation with Pediococcus pentosaceus enhances the innate immune response in and promotes growth of Litopenaeus vannamei shrimp

Abstract

To reach the sustainable development goals on health management in Litopenaeus vannamei shrimp culture, Pediococcus pentosaceus AB01 was supplemented in shrimp diet. In this study, the control diet and three experimental diets containing P. pentosaceus AB01 (108, 109, 1010 CFU/g) were separately introduced to L. vannamei for a 28 days feeding trial. After the feeding trial, percent weight gain, feeding efficiency, and feed conversion ratio (FCR) were significantly elevated in L. vannamei administered with P. pentosaceus AB01 at 109 and 1010 colony‐forming unit (CFU)/g. Protease, amylase, and trypsin were found at higher levels in the probiotic‐supplied groups. The feeding of shrimps with P. pentosaceus AB01 significantly increased innate immune response and levels of related biochemical parameters in the haemolymph. After the white spot syndrome virus (WSSV) challenge, supplementation of P. pentosaceus AB01 had significant positive effects (p < .05) on survival rate, compared to that of the control diet. The higher resistance of L. vannamei to WSSV might have been due to alterations in the gut microbiome composition and upregulation of the Toll‐Like Receptor (TLR) signalling pathway. Hence, P. pentosaceus AB01 may be a promising alternative feed to promote growth rate, modulate microbiota composition, and enhance immunity in L. vannamei shrimp.

Isolation of Pediococcus pentosaceus to compete Vibrio harveyi in the shrimp Litopenaeus vannamei hatchery

“…The current study showed that Pediococcus pentosaceus at a dose of 5-10 µL L -1 can increase survival rate, MBW, and biomass and can reduce YVC, GVC, and TVC in the early phase (M 3) and late period of infection (PL 10) by V. harveyi, although it was not statistically significant. Previous research conducted by Le et al (2022) showed that the addition of P. pentosaceus in shrimp feed could enhance the immunity of whiteleg shrimp. Adel et al (2016), Won et al (2020), andThao et al (2021) also reported that a concentration of 10 8 CFU g -1 of P. pentosaceus to shrimp increased survival rate, growth performance, digestive enzyme activity, immunity, histological conditions of the digestive tract, immune gene expression, and their tolerance to V. anguillarum and V. parahaemolyticus infections and was able to reduce TVC.…”

Pediococcus pentosaceus MIANGUAN2 Alleviates Influenza Virus Infection by Modulating Gut Microbiota and Enhancing Short-Chain Fatty Acid Production

“…The probiotics, mainly those of the lactobacillus and bifidobaterium genera, such as Lactobacillus plantarum [ 53 , 54 ], Lactobacillus rhamnosus GG [ 55 ], Lactobacillus paracasei [ 23 ], Bifdobacterium bifdum [ 56 ], and Bifidobacterium animalis [ 16 ], can combat the influenza virus infection through regulating the immune response. The probiotic of Pediococcus pentosaceus has been shown to have immunomodulatory effects [ 30 , 31 , 32 ]. However, it is not known if it has an anti-influenza effect.…”

“…Pediococcus pentosaceus is Gram-positive facultative anaerobic lactic acid bacteria, which has several beneficial properties, such as antimicrobial [ 24 ], detoxifying [ 25 ], lipid-lowering [ 26 ], anticarcinogenic [ 27 ], intestinal disease-alleviating [ 28 , 29 ], and immune-enhancing activities [ 30 , 31 , 32 ]. However, it is unknown whether oral administration with P. pentosaceus is effective against respiratory viral infections.…”