Bacillus solani sp. nov., isolated from rhizosphere soil of a potato field

Liu, Bo; Liu, Guo-Hong; Şengonça, Ç.; Schümann, Peter; Ge, Changwei; Wang, Jieping; Cui, Wei-Dong; Lin, Nankai

doi:10.1099/ijsem.0.000539

Cited by 15 publications

(8 citation statements)

References 30 publications

(28 reference statements)

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“…The main isoprenoid quinone of strain 03113 T was identified as MK-7. The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, three unknown aminophospholipids, and one unknown phospholipid, which was consistent with the predominant component of B. solani CCTCC AB 2014277 T [7]. The major fatty acids of strain 03113 T were anteiso-C 15:0 (51.35%), iso-C 15:0 (11.06%), and iso-C 14:0 (7.13%), which were similar to those of the reference strains (Table 2).…”

Section: Analysis Of Isoprenoid Quinones Polar Lipids and Cellular Fatty Acidssupporting

confidence: 52%

“…Strain 03113 T was isolated from greenhouse soil from Wangsiying, Chaoyang District, Beijing, China (40 • 09 N, 116 • 42 E). We preserved the sample in freeze-dried milk ampoules at 4 • C and 20% (v/v) glycerol at −80 • C [7]. The type strains of species closely related to strain 03113 T were used as reference strains under the same conditions for comparative taxonomic analysis, which included B. solani CCTCC AB 2014277 T , B. praedii CCTCC AB 2015208 T , and B. dabaoshanensis CCTCC AB 2013260 T .…”

Section: Bacterial Strains Growth Conditions and Cultivationmentioning

confidence: 99%

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Bacillus telluris sp. nov. Isolated from Greenhouse Soil in Beijing, China

Guo

Wang

et al. 2020

Microorganisms

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A novel Gram-stain-positive, rod-shaped, endospore-forming bacterium, which we designated as strain 03113T, was isolated from greenhouse soil in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed strain 03113T is in the genus Bacillus and had the highest similarity to Bacillus solani CCTCC AB 2014277T (98.14%). The strain grew at 4 °C–50 °C (optimum 37 °C), with 0–10% (w/v) NaCl (optimum 5%), and in the range of pH 3.0–12.0 (optimum pH 8.0). Menaquinone was identified as MK-7, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The main major cellular fatty acids detected were anteiso-C15:0 (51.35%) and iso-C15:0 (11.06%), which are the predominant cellular fatty acids found in all recognized members of the genus Bacillus. The 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANI), and in silico DNA—DNA hybridization (DDH) value between strain 03113T and the most closely related species were 70.5% and 22.6%, respectively, which supported our conclusion that 03113T represented a novel species in the genus Bacillus. We demonstrated that type strain 03113T (=ACCC 03113T=JCM 33017T) was a novel species in the genus Bacillus, and the name Bacillus telluris sp. nov. was proposed. Strain 03113T secreted auxin IAA and carried the nitrogenase iron protein (nifH) gene, which indicated that strain 03113T has the potential to fix nitrogen and promote plant growth. Bacillus telluris sp. nov. 03113T is a potential candidate for the biofertilizers of organic agriculture areas.

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Section: Analysis Of Isoprenoid Quinones Polar Lipids and Cellular Fatty Acidssupporting

confidence: 52%

Section: Bacterial Strains Growth Conditions and Cultivationmentioning

confidence: 99%

Bacillus telluris sp. nov. Isolated from Greenhouse Soil in Beijing, China

Guo

Wang

et al. 2020

Microorganisms

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“…Another opportunistic endophyte, B. kochii reported in the present work was first isolated from dairy and pharmaceutical production sites (Seiler et al, 2012) and later reported from soil (Liu et al, 2020), compost pit, coral reef, insect gut (Wan et al, 2017), fermented fish (Alvionita et al, 2020) and sparingly found as endophytes in tomato seed (Someya et al, 2021), papaya (Thomas et al, 2019) and noni (Liu et al, 2015b). Bacillus solani , designated as passenger endophyte of the present study, was previously reported as potato rhizosphere colonizing antagonistic bacteria (Liu et al, 2015a). Another passenger endophyte, Micrococcus luteus , was also found in diverse ecological niches like human skin (Kloos & Musselwhite, 1975), soil (Sims et al, 1986), indoor air samples (Kutmutia et al, 2019) and was occasionally reported as an endophyte of jute (Haidar et al, 2018) and sorghum root (Gamalero et al, 2020).…”

Section: Discussionmentioning

confidence: 57%

Authentication of putative competitive bacterial endophytes of rice by re-isolation and DNA fingerprinting assay

Devi

Balachandar

2022

Journal of Applied Microbiology

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Aim:The plant-growth-promoting putative competitive endophytes offer significant benefits to sustainable agriculture. The unworthy opportunistic and passenger endophytes are inevitable during the isolation of putative competitive endophytes.This study aimed to discriminate the putative competitive endophytes undoubtedly from the opportunistic and passenger endophytes. Methods and results:The newly isolated endophytes from field-grown rice were inoculated to 5-days old rice seedlings under gnotobiotic conditions. Re-isolation of the inoculated strains from the root surface, inner tissues of the whole plant, root and shoot was performed after 5-days. All the re-isolated colonies were compared with native isolates for homology by BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) DNA fingerprints. The results revealed that the putative competitive endophytes (RE25 and RE10) showed positive for re-isolation and BOX and ERIC fingerprints for the whole plant, root and shoot. The opportunistic (RE27 and RE8) and passenger endophytes (RE44 and RE18) failed in re-isolation either from root or shoot. The epiphytes (ZSB15 and Az204) showed negative for endophytic re-isolation and positive for surface colonization. Conclusion:This modified procedure can discriminate the putative competitive endophytes from others.Significance and impact of the study: Eliminating the opportunistic and passenger endophytes and epiphytes early by this method would help develop endophytic inoculants to enhance rice productivity.

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“…Menaquinone-7 (MK-7) was identified in strain FJAT-49705 T , which was similar to the members of genus Cytobacillus [5–7]. The major fatty acids of strain FJAT-49705 T were anteiso-C 15 : 0 and iso-C 15 : 0 .…”

Section: Full-textmentioning

confidence: 99%

“…The polar lipids of strain FJAT-49705 T (Fig. S3) were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and an unidentified phospholipid, whereas 'B, dafuensis' , C. solani and C. praedii had DPG, phosphatidylglycerol and PE [4,6,30].…”

mentioning

confidence: 99%

Cytobacillus citreus sp. nov., isolated from citrus rhizosphere soil

Liu

Rao

Liu

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample. Strain FJAT-49705T grew at 20–40 °C (optimum, 30 °C) and pH 6.0–11.0 (optimum, pH 7.0) with 0–5 % (w/v) NaCl (optimum, 2 %). Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to ‘Bacillus dafuensis’ FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %). In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus . MK-7 was the only isoprenoid quinone present. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The genomic DNA G+C content was 36.9 %. The average nucleotide identity (ANI) values between FJAT-49705T and ‘B. dafuensis’ FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95–96 %) recommended as the ANI criterion for interspecies identity. Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus , for which the name Cytobacillus citreus sp. nov. is proposed. The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).

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Bacillus solani sp. nov., isolated from rhizosphere soil of a potato field

Cited by 15 publications

References 30 publications

Bacillus telluris sp. nov. Isolated from Greenhouse Soil in Beijing, China

Bacillus telluris sp. nov. Isolated from Greenhouse Soil in Beijing, China

Authentication of putative competitive bacterial endophytes of rice by re-isolation and DNA fingerprinting assay

Cytobacillus citreus sp. nov., isolated from citrus rhizosphere soil

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