Bacillus telluris sp. nov. Isolated from Greenhouse Soil in Beijing, China

Abstract

A novel Gram-stain-positive, rod-shaped, endospore-forming bacterium, which we designated as strain 03113T, was isolated from greenhouse soil in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed strain 03113T is in the genus Bacillus and had the highest similarity to Bacillus solani CCTCC AB 2014277T (98.14%). The strain grew at 4 °C–50 °C (optimum 37 °C), with 0–10% (w/v) NaCl (optimum 5%), and in the range of pH 3.0–12.0 (optimum pH 8.0). Menaquinone was identified as MK-7, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The main major cellular fatty acids detected were anteiso-C15:0 (51.35%) and iso-C15:0 (11.06%), which are the predominant cellular fatty acids found in all recognized members of the genus Bacillus. The 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANI), and in silico DNA—DNA hybridization (DDH) value between strain 03113T and the most closely related species were 70.5% and 22.6%, respectively, which supported our conclusion that 03113T represented a novel species in the genus Bacillus. We demonstrated that type strain 03113T (=ACCC 03113T=JCM 33017T) was a novel species in the genus Bacillus, and the name Bacillus telluris sp. nov. was proposed. Strain 03113T secreted auxin IAA and carried the nitrogenase iron protein (nifH) gene, which indicated that strain 03113T has the potential to fix nitrogen and promote plant growth. Bacillus telluris sp. nov. 03113T is a potential candidate for the biofertilizers of organic agriculture areas.

Metabacillus dongyingensis sp. nov. Is Represented by the Plant Growth-Promoting Bacterium BY2G20 Isolated from Saline-Alkaline Soil and Enhances the Growth of Zea mays L. under Salt Stress

“…The genus Bacillus comprises typical species of PGPRs that are applied as biofertilizers and competitors of plant pathogens to enhance plant growth, for example, by promoting seedling emergence, enhancing plant biomass, and controlling disease ( 7 ). Furthermore, members of the genus Bacillus are widely distributed in the air, soil, and water, and have a wide range of physiological and biochemical characteristics from psychrophilic to thermophilic, acidophilic to alkaliphilic, and halophilic ( 8 ). Several alkaliphilic, alkalitolerant, or halophilic species of Bacillus have been isolated from different saline-alkaline habitats.…”

Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics

“…In particular, it has been shown that bacteria are the first organisms to colonize these environments through metabolic adaptations [ 57 ], which increase the possibility of colonization by species that act as biological stabilizers (fungi, lichens, and mosses), which could also be an important niche for seed germination or the establishment of plant species [ 58 ]. Generally, bacteria with PGP capabilities have been isolated from crops with agronomic interest under stable environmental conditions [ 59 ] in order to improve crop yield [ 60 , 61 ], while PGP bacteria isolated from extreme environments are relatively unexplored [ 62 ]. In an era of climate change, the identification, isolation, and characterization of bacteria with PGP properties that come from extreme environments that may favor plant growth under abiotic stress conditions represent excellent and novel biotechnological tools to fight future harsh conditions [ 30 , 63 ].…”

Screening of Indigenous Microorganisms as Potential Biofertilisers for Periurban Horticulture Areas

“…On the other hand, it is known that PGPR strains may produce siderophores with high Fe-complexing capacity causing an antagonist action against phytopathogenic micro or macroorganisms [9,11]. As example, bacteria have been found to assist in root colonization by Rhizobia suppressing possible actions of plant pathogens, especially Pseudomonas and Bacilli behaving as PGPR associated to the rhizosphere of various leguminous crops [12,13].…”